O35025 (YDIR_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme BsuMI component YdiR
Short name=R.BsuM
Short name=R.BsuMI
EC=3.1.21.4

Alternative name(s):
Endonuclease BsuMI component YdiR
Type II restriction enzyme BsuMI component YdiR

Gene names

Name:	ydiR
Ordered Locus Names:	BSU06090

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Subunit structure	BsuMI restriction activity requires YdiR, YdiS and YdjA.
Developmental stage	Not expressed during sporulation. Ref.4
Induction	Constitutively expressed during exponential growth. Encoded in an operon with ydiS and ydjA. Ref.4
Disruption phenotype	Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences. Ref.4

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Type-2 restriction enzyme BsuMI component YdiR		PRO_0000049509

Sequences

	Sequence		Length	Mass (Da)
	O35025 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5825E8C93A8141AE	FASTA	313	35,311
	10 20 30 40 50 60 MTFIKRLEDA YETLLGNYPA GVSSTSTSKY NEIRKIVSEA FLIGENEVYV TGTSRRISNL 70 80 90 100 110 120 DTRFAQGNQR NKHTRMAVAF ISIPSVDDSE LDELIIRTRN SAITTSSKFC NGEERGTIFD 130 140 150 160 170 180 GILLFLVFEG ETKVYPLAFL VFENDFELKE KAEELIPGIE LKEYPRANQS PAQENNKSAK 190 200 210 220 230 240 NEDEESAKSY VVFLDIEEDG SIVEFVEDKD KTYRIGDMIW TASHTNGSSA ITRRLEVIEV 250 260 270 280 290 300 VENLVVCKIK HKYNEPVDKN SLLKFVNIEQ DLISFLDLHP NVQNGSEGFV SGDIVDENAT 310 TSSDDLPEDF ENN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes." Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y. DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation." Bron S., Janniere L., Ehrlich S.D. Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract] Cited for: DNA TARGET SEQUENCE. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]	"Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg." Ohshima H., Matsuoka S., Asai K., Sadaie Y. J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007637 Genomic DNA. Translation: BAA22753.1. AL009126 Genomic DNA. Translation: CAB12428.1.
PIR	C69788.
RefSeq	NP_388490.1. NC_000964.3.
3D structure databases
ProteinModelPortal	O35025.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU06090.
Protein family/group databases
REBASE	619. BsuMI.
Proteomic databases
PaxDb	O35025.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB12428; CAB12428; BSU06090.
GeneID	938002.
KEGG	bsu:BSU06090.
PATRIC	18972854. VBIBacSub10457_0641.
Organism-specific databases
GenoList	BSU06090.
Enzyme and pathway databases
BioCyc	BSUB:BSU06090-MONOMER.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

YDIR_BACSU

Accession

Primary (citable) accession number: O35025

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program