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O35025 (YDIR_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsuMI component YdiR

Short name=R.BsuM
Short name=R.BsuMI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsuMI component YdiR
Type II restriction enzyme BsuMI component YdiR
Gene names
Name:ydiR
Ordered Locus Names:BSU06090
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

BsuMI restriction activity requires YdiR, YdiS and YdjA.

Developmental stage

Not expressed during sporulation. Ref.4

Induction

Constitutively expressed during exponential growth. Encoded in an operon with ydiS and ydjA. Ref.4

Disruption phenotype

Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences. Ref.4

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Type-2 restriction enzyme BsuMI component YdiR
PRO_0000049509

Sequences

Sequence LengthMass (Da)Tools
O35025 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 5825E8C93A8141AE

FASTA31335,311
        10         20         30         40         50         60 
MTFIKRLEDA YETLLGNYPA GVSSTSTSKY NEIRKIVSEA FLIGENEVYV TGTSRRISNL 

        70         80         90        100        110        120 
DTRFAQGNQR NKHTRMAVAF ISIPSVDDSE LDELIIRTRN SAITTSSKFC NGEERGTIFD 

       130        140        150        160        170        180 
GILLFLVFEG ETKVYPLAFL VFENDFELKE KAEELIPGIE LKEYPRANQS PAQENNKSAK 

       190        200        210        220        230        240 
NEDEESAKSY VVFLDIEEDG SIVEFVEDKD KTYRIGDMIW TASHTNGSSA ITRRLEVIEV 

       250        260        270        280        290        300 
VENLVVCKIK HKYNEPVDKN SLLKFVNIEQ DLISFLDLHP NVQNGSEGFV SGDIVDENAT 

       310 
TSSDDLPEDF ENN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
Bron S., Janniere L., Ehrlich S.D.
Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA TARGET SEQUENCE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
Ohshima H., Matsuoka S., Asai K., Sadaie Y.
J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007637 Genomic DNA. Translation: BAA22753.1.
AL009126 Genomic DNA. Translation: CAB12428.1.
PIRC69788.
RefSeqNP_388490.1. NC_000964.3.

3D structure databases

ProteinModelPortalO35025.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU06090.

Protein family/group databases

REBASE619. BsuMI.

Proteomic databases

PaxDbO35025.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12428; CAB12428; BSU06090.
GeneID938002.
KEGGbsu:BSU06090.
PATRIC18972854. VBIBacSub10457_0641.

Organism-specific databases

GenoListBSU06090.

Phylogenomic databases

OMAKEYPRAN.

Enzyme and pathway databases

BioCycBSUB:BSU06090-MONOMER.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameYDIR_BACSU
AccessionPrimary (citable) accession number: O35025
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList