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O35025

- YDIR_BACSU

UniProt

O35025 - YDIR_BACSU

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Protein

Type-2 restriction enzyme BsuMI component YdiR

Gene

ydiR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BioCyciBSUB:BSU06090-MONOMER.

Protein family/group databases

REBASEi619. BsuMI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuMI component YdiR (EC:3.1.21.4)
Short name:
R.BsuM
Short name:
R.BsuMI
Alternative name(s):
Endonuclease BsuMI component YdiR
Type II restriction enzyme BsuMI component YdiR
Gene namesi
Name:ydiR
Ordered Locus Names:BSU06090
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU06090.

Pathology & Biotechi

Disruption phenotypei

Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Type-2 restriction enzyme BsuMI component YdiRPRO_0000049509Add
BLAST

Proteomic databases

PaxDbiO35025.

Expressioni

Developmental stagei

Not expressed during sporulation.1 Publication

Inductioni

Constitutively expressed during exponential growth. Encoded in an operon with ydiS and ydjA.1 Publication

Interactioni

Subunit structurei

BsuMI restriction activity requires YdiR, YdiS and YdjA.

Protein-protein interaction databases

STRINGi224308.BSU06090.

Structurei

3D structure databases

ProteinModelPortaliO35025.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

OMAiKEYPRAN.

Sequencei

Sequence statusi: Complete.

O35025-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTFIKRLEDA YETLLGNYPA GVSSTSTSKY NEIRKIVSEA FLIGENEVYV
60 70 80 90 100
TGTSRRISNL DTRFAQGNQR NKHTRMAVAF ISIPSVDDSE LDELIIRTRN
110 120 130 140 150
SAITTSSKFC NGEERGTIFD GILLFLVFEG ETKVYPLAFL VFENDFELKE
160 170 180 190 200
KAEELIPGIE LKEYPRANQS PAQENNKSAK NEDEESAKSY VVFLDIEEDG
210 220 230 240 250
SIVEFVEDKD KTYRIGDMIW TASHTNGSSA ITRRLEVIEV VENLVVCKIK
260 270 280 290 300
HKYNEPVDKN SLLKFVNIEQ DLISFLDLHP NVQNGSEGFV SGDIVDENAT
310
TSSDDLPEDF ENN
Length:313
Mass (Da):35,311
Last modified:January 1, 1998 - v1
Checksum:i5825E8C93A8141AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007637 Genomic DNA. Translation: BAA22753.1.
AL009126 Genomic DNA. Translation: CAB12428.1.
PIRiC69788.
RefSeqiNP_388490.1. NC_000964.3.
WP_003244216.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB12428; CAB12428; BSU06090.
GeneIDi938002.
KEGGibsu:BSU06090.
PATRICi18972854. VBIBacSub10457_0641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007637 Genomic DNA. Translation: BAA22753.1 .
AL009126 Genomic DNA. Translation: CAB12428.1 .
PIRi C69788.
RefSeqi NP_388490.1. NC_000964.3.
WP_003244216.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali O35025.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU06090.

Protein family/group databases

REBASEi 619. BsuMI.

Proteomic databases

PaxDbi O35025.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB12428 ; CAB12428 ; BSU06090 .
GeneIDi 938002.
KEGGi bsu:BSU06090.
PATRICi 18972854. VBIBacSub10457_0641.

Organism-specific databases

GenoListi BSU06090.

Phylogenomic databases

OMAi KEYPRAN.

Enzyme and pathway databases

BioCyci BSUB:BSU06090-MONOMER.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
    Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
    DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
    Bron S., Janniere L., Ehrlich S.D.
    Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA TARGET SEQUENCE.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  4. "Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
    Ohshima H., Matsuoka S., Asai K., Sadaie Y.
    J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Entry informationi

Entry nameiYDIR_BACSU
AccessioniPrimary (citable) accession number: O35025
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

External Data

Dasty 3