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Protein

Low molecular weight protein-tyrosine-phosphatase YfkJ

Gene

yfkJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins. Involved in ethanol stress resistance.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Efficiently inhibited by Cu2+ ion, Zn2+ ion and N-ethylmaleimide, while the addition of Mg2+, Ca2+ or Fe3+ ions has minimal effect. Inhibited in a competitive manner by vanadate.1 Publication

Kineticsi

  1. KM=244 µM for p-nitrophenyl-phosphate (at 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei8Nucleophile1
    Active sitei141
    Active sitei125Proton donor1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciBSUB:BSU07880-MONOMER.
    SABIO-RKO35016.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight protein-tyrosine-phosphatase YfkJ (EC:3.1.3.48)
    Short name:
    LMPTP
    Gene namesi
    Name:yfkJ
    Ordered Locus Names:BSU07880
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi8C → S: Completely abolishes the tyrosine-phosphatase activity. 1 Publication1
    Mutagenesisi14R → K: Completely abolishes the tyrosine-phosphatase activity. 1 Publication1
    Mutagenesisi125D → A: Completely abolishes the tyrosine-phosphatase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002346591 – 156Low molecular weight protein-tyrosine-phosphatase YfkJAdd BLAST156

    Proteomic databases

    PaxDbiO35016.

    Expressioni

    Inductioni

    By ethanol stress. Expression is sigma B-dependent.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100004383.

    Structurei

    Secondary structure

    1156
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 13Combined sources12
    Helixi14 – 28Combined sources15
    Turni32 – 34Combined sources3
    Beta strandi35 – 43Combined sources9
    Turni45 – 48Combined sources4
    Helixi53 – 61Combined sources9
    Helixi76 – 81Combined sources6
    Beta strandi83 – 89Combined sources7
    Helixi90 – 100Combined sources11
    Beta strandi108 – 110Combined sources3
    Helixi111 – 114Combined sources4
    Helixi126 – 129Combined sources4
    Helixi132 – 153Combined sources22

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4ETMX-ray1.60A/B1-156[»]
    ProteinModelPortaliO35016.
    SMRiO35016.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105KJF. Bacteria.
    COG0394. LUCA.
    HOGENOMiHOG000273094.
    InParanoidiO35016.
    KOiK01104.
    OMAiWHIGKKA.
    PhylomeDBiO35016.

    Family and domain databases

    CDDicd00115. LMWPc. 1 hit.
    InterProiIPR023485. Ptyr_pPase_SF.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O35016-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MISVLFVCLG NICRSPMAEA IFRDLAAKKG LEGKIKADSA GIGGWHIGNP
    60 70 80 90 100
    PHEGTQEILR REGISFDGML ARQVSEQDLD DFDYIIAMDA ENIGSLRSMA
    110 120 130 140 150
    GFKNTSHIKR LLDYVEDSDL ADVPDPYYTG NFEEVCQLIK TGCEQLLASI

    QKEKQL
    Length:156
    Mass (Da):17,269
    Last modified:January 1, 1998 - v1
    Checksum:i7CDC8C4D6D0FD528
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL009126 Genomic DNA. Translation: CAB12617.1.
    D83967 Genomic DNA. Translation: BAA23400.1.
    PIRiE69808.
    RefSeqiNP_388669.1. NC_000964.3.
    WP_003243480.1. NZ_JNCM01000032.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12617; CAB12617; BSU07880.
    GeneIDi939191.
    KEGGibsu:BSU07880.
    PATRICi18973226. VBIBacSub10457_0827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL009126 Genomic DNA. Translation: CAB12617.1.
    D83967 Genomic DNA. Translation: BAA23400.1.
    PIRiE69808.
    RefSeqiNP_388669.1. NC_000964.3.
    WP_003243480.1. NZ_JNCM01000032.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4ETMX-ray1.60A/B1-156[»]
    ProteinModelPortaliO35016.
    SMRiO35016.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100004383.

    Proteomic databases

    PaxDbiO35016.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB12617; CAB12617; BSU07880.
    GeneIDi939191.
    KEGGibsu:BSU07880.
    PATRICi18973226. VBIBacSub10457_0827.

    Phylogenomic databases

    eggNOGiENOG4105KJF. Bacteria.
    COG0394. LUCA.
    HOGENOMiHOG000273094.
    InParanoidiO35016.
    KOiK01104.
    OMAiWHIGKKA.
    PhylomeDBiO35016.

    Enzyme and pathway databases

    BioCyciBSUB:BSU07880-MONOMER.
    SABIO-RKO35016.

    Family and domain databases

    CDDicd00115. LMWPc. 1 hit.
    InterProiIPR023485. Ptyr_pPase_SF.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYFKJ_BACSU
    AccessioniPrimary (citable) accession number: O35016
    Secondary accession number(s): Q79EX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: January 1, 1998
    Last modified: November 2, 2016
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.