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O35016

- YFKJ_BACSU

UniProt

O35016 - YFKJ_BACSU

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Protein
Low molecular weight protein-tyrosine-phosphatase YfkJ
Gene
yfkJ, BSU07880
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Dephosphorylates the phosphotyrosine-containing proteins. Involved in ethanol stress resistance.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Efficiently inhibited by Cu2+ ion, Zn2+ ion and N-ethylmaleimide, while the addition of Mg2+, Ca2+ or Fe3+ ions has minimal effect. Inhibited in a competitive manner by vanadate.1 Publication

Kineticsi

  1. KM=244 µM for p-nitrophenyl-phosphate (at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 6.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81Nucleophile
Active sitei14 – 141
Active sitei125 – 1251Proton donor

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciBSUB:BSU07880-MONOMER.
    RETL1328306-WGS:GSTH-4023-MONOMER.
    SABIO-RKiO35016.

    Protein family/group databases

    PptaseDBiP3D0507171.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight protein-tyrosine-phosphatase YfkJ (EC:3.1.3.48)
    Short name:
    LMPTP
    Gene namesi
    Name:yfkJ
    Ordered Locus Names:BSU07880
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU07880.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi8 – 81C → S: Completely abolishes the tyrosine-phosphatase activity. 1 Publication
    Mutagenesisi14 – 141R → K: Completely abolishes the tyrosine-phosphatase activity. 1 Publication
    Mutagenesisi125 – 1251D → A: Completely abolishes the tyrosine-phosphatase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 156156Low molecular weight protein-tyrosine-phosphatase YfkJ
    PRO_0000234659Add
    BLAST

    Proteomic databases

    PaxDbiO35016.

    Expressioni

    Inductioni

    By ethanol stress. Expression is sigma B-dependent.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU07880.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312
    Helixi14 – 2815
    Turni32 – 343
    Beta strandi35 – 439
    Turni45 – 484
    Helixi53 – 619
    Helixi76 – 816
    Beta strandi83 – 897
    Helixi90 – 10011
    Beta strandi108 – 1103
    Helixi111 – 1144
    Helixi126 – 1294
    Helixi132 – 15322

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4ETMX-ray1.60A/B1-156[»]
    ProteinModelPortaliO35016.
    SMRiO35016. Positions 2-150.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0394.
    HOGENOMiHOG000273094.
    KOiK01104.
    OMAiDSAGTHD.
    OrthoDBiEOG6MH5JB.
    PhylomeDBiO35016.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O35016-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISVLFVCLG NICRSPMAEA IFRDLAAKKG LEGKIKADSA GIGGWHIGNP    50
    PHEGTQEILR REGISFDGML ARQVSEQDLD DFDYIIAMDA ENIGSLRSMA 100
    GFKNTSHIKR LLDYVEDSDL ADVPDPYYTG NFEEVCQLIK TGCEQLLASI 150
    QKEKQL 156
    Length:156
    Mass (Da):17,269
    Last modified:January 1, 1998 - v1
    Checksum:i7CDC8C4D6D0FD528
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB12617.1.
    D83967 Genomic DNA. Translation: BAA23400.1.
    PIRiE69808.
    RefSeqiNP_388669.1. NC_000964.3.
    WP_003243480.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12617; CAB12617; BSU07880.
    GeneIDi939191.
    KEGGibsu:BSU07880.
    PATRICi18973226. VBIBacSub10457_0827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB12617.1 .
    D83967 Genomic DNA. Translation: BAA23400.1 .
    PIRi E69808.
    RefSeqi NP_388669.1. NC_000964.3.
    WP_003243480.1. NZ_CM000487.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4ETM X-ray 1.60 A/B 1-156 [» ]
    ProteinModelPortali O35016.
    SMRi O35016. Positions 2-150.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU07880.

    Protein family/group databases

    PptaseDBi P3D0507171.

    Proteomic databases

    PaxDbi O35016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12617 ; CAB12617 ; BSU07880 .
    GeneIDi 939191.
    KEGGi bsu:BSU07880.
    PATRICi 18973226. VBIBacSub10457_0827.

    Organism-specific databases

    GenoListi BSU07880.

    Phylogenomic databases

    eggNOGi COG0394.
    HOGENOMi HOG000273094.
    KOi K01104.
    OMAi DSAGTHD.
    OrthoDBi EOG6MH5JB.
    PhylomeDBi O35016.

    Enzyme and pathway databases

    BioCyci BSUB:BSU07880-MONOMER.
    RETL1328306-WGS:GSTH-4023-MONOMER.
    SABIO-RKi O35016.

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    2. "Nucleotide sequence analysis of B. subtilis cromosome in 74 degree region."
      Sekiguchi J., Yamamoto H., Uchiyama S., Fajar A.
      Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / AC327.
    3. "Low-molecular-weight protein tyrosine phosphatases of Bacillus subtilis."
      Musumeci L., Bongiorni C., Tautz L., Edwards R.A., Osterman A., Perego M., Mustelin T., Bottini N.
      J. Bacteriol. 187:4945-4956(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF CYS-8; ARG-14 AND ASP-125, CHARACTERIZATION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: 168 / JH642.

    Entry informationi

    Entry nameiYFKJ_BACSU
    AccessioniPrimary (citable) accession number: O35016
    Secondary accession number(s): Q79EX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: January 1, 1998
    Last modified: September 3, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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