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Protein

Glucosamine-6-phosphate deaminase 1

Gene

nagB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.By similarity

Catalytic activityi

Alpha-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3.

Pathwayi: N-acetylneuraminate degradation

This protein is involved in step 5 of the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. N-acetylglucosamine-6-phosphate deacetylase (nagA)
  5. Glucosamine-6-phosphate deaminase 1 (nagB), Probable glucosamine-6-phosphate deaminase 2 (gamA)
This subpathway is part of the pathway N-acetylneuraminate degradation, which is itself part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate, the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Proton acceptor; for enolization stepBy similarity
Active sitei136 – 1361For ring-opening stepBy similarity
Active sitei138 – 1381Proton acceptor; for ring-opening stepBy similarity
Active sitei143 – 1431For ring-opening stepBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBSUB:BSU35020-MONOMER.
SABIO-RKO35000.
UniPathwayiUPA00629; UER00684.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosamine-6-phosphate deaminase 1 (EC:3.5.99.6)
Alternative name(s):
GlcN6P deaminase 1
Short name:
GNPDA 1
Glucosamine-6-phosphate isomerase 1
Gene namesi
Name:nagB
Ordered Locus Names:BSU35020
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 242242Glucosamine-6-phosphate deaminase 1PRO_0000160133Add
BLAST

Proteomic databases

PaxDbiO35000.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018956.

Structurei

Secondary structure

1
242
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi9 – 2618Combined sources
Beta strandi31 – 344Combined sources
Helixi41 – 5313Combined sources
Beta strandi62 – 698Combined sources
Helixi80 – 878Combined sources
Helixi89 – 913Combined sources
Helixi96 – 983Combined sources
Helixi109 – 12214Combined sources
Beta strandi127 – 1315Combined sources
Beta strandi152 – 1565Combined sources
Helixi159 – 1657Combined sources
Helixi166 – 1683Combined sources
Beta strandi169 – 1713Combined sources
Helixi172 – 1743Combined sources
Beta strandi177 – 1815Combined sources
Helixi184 – 1885Combined sources
Beta strandi193 – 1975Combined sources
Helixi200 – 2023Combined sources
Helixi203 – 2119Combined sources
Helixi219 – 2257Combined sources
Beta strandi227 – 2348Combined sources
Turni235 – 2406Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKVX-ray1.50A/B1-242[»]
2BKXX-ray1.40A/B1-242[»]
ProteinModelPortaliO35000.
SMRiO35000. Positions 1-242.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35000.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CKA. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
InParanoidiO35000.
KOiK02564.
OMAiPIDLCCL.
PhylomeDBiO35000.

Family and domain databases

HAMAPiMF_01241. GlcN6P_deamin. 1 hit.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVMECQTYE ELSQIAARIT ADTIKEKPDA VLGLATGGTP EGTYRQLIRL
60 70 80 90 100
HQTENLSFQN ITTVNLDEYA GLSSDDPNSY HFYMNDRFFQ HIDSKPSRHF
110 120 130 140 150
IPNGNADDLE AECRRYEQLV DSLGDTDIQL LGIGRNGHIG FNEPGTSFKS
160 170 180 190 200
RTHVVTLNEQ TRQANARYFP SIDSVPKKAL TMGIQTILSS KRILLLISGK
210 220 230 240
SKAEAVRKLL EGNISEDFPA SALHLHSDVT VLIDREAASL RP
Length:242
Mass (Da):26,991
Last modified:January 1, 1998 - v1
Checksum:iB7979780B8E35C66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67284.1.
AL009126 Genomic DNA. Translation: CAB15507.1.
PIRiB69664.
RefSeqiNP_391382.1. NC_000964.3.
WP_003228092.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15507; CAB15507; BSU35020.
GeneIDi936619.
KEGGibsu:BSU35020.
PATRICi18979010. VBIBacSub10457_3667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67284.1.
AL009126 Genomic DNA. Translation: CAB15507.1.
PIRiB69664.
RefSeqiNP_391382.1. NC_000964.3.
WP_003228092.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKVX-ray1.50A/B1-242[»]
2BKXX-ray1.40A/B1-242[»]
ProteinModelPortaliO35000.
SMRiO35000. Positions 1-242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018956.

Proteomic databases

PaxDbiO35000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15507; CAB15507; BSU35020.
GeneIDi936619.
KEGGibsu:BSU35020.
PATRICi18979010. VBIBacSub10457_3667.

Phylogenomic databases

eggNOGiENOG4105CKA. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
InParanoidiO35000.
KOiK02564.
OMAiPIDLCCL.
PhylomeDBiO35000.

Enzyme and pathway databases

UniPathwayiUPA00629; UER00684.
BioCyciBSUB:BSU35020-MONOMER.
SABIO-RKO35000.

Miscellaneous databases

EvolutionaryTraceiO35000.

Family and domain databases

HAMAPiMF_01241. GlcN6P_deamin. 1 hit.
InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00502. nagB. 1 hit.
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAGB_BACSU
AccessioniPrimary (citable) accession number: O35000
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.