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Protein

DNA polymerase I

Gene

polA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication

Enzyme and pathway databases

BioCyciBSUB:BSU29090-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Ordered Locus Names:BSU29090
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012351 – 880DNA polymerase IAdd BLAST880

Proteomic databases

PaxDbiO34996
PRIDEiO34996

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.

Protein-protein interaction databases

IntActiO34996, 8 interactors
STRINGi224308.Bsubs1_010100015876

Structurei

3D structure databases

ProteinModelPortaliO34996
SMRiO34996
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini174 – 2685'-3' exonucleaseSequence analysisAdd BLAST95
Domaini302 – 4703'-5' exonucleaseSequence analysisAdd BLAST169

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

eggNOGiENOG4105C2M Bacteria
COG0258 LUCA
COG0749 LUCA
HOGENOMiHOG000020998
InParanoidiO34996
KOiK02335
OMAiETGRVHT
PhylomeDBiO34996

Family and domain databases

CDDicd09898 H3TH_53EXO, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR002562 3'-5'_exonuclease_dom
IPR020046 5-3_exonucl_a-hlix_arch_N
IPR036279 5-3_exonuclease_C_sf
IPR002421 5-3_exonuclease_N
IPR019760 DNA-dir_DNA_pol_A_CS
IPR001098 DNA-dir_DNA_pol_A_palm_dom
IPR020045 DNA_polI_H3TH
IPR018320 DNA_polymerase_1
IPR002298 DNA_polymerase_A
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10133 PTHR10133, 1 hit
PfamiView protein in Pfam
PF01367 5_3_exonuc, 1 hit
PF02739 5_3_exonuc_N, 1 hit
PF00476 DNA_pol_A, 1 hit
PRINTSiPR00868 DNAPOLI
SMARTiView protein in SMART
SM00474 35EXOc, 1 hit
SM00475 53EXOc, 1 hit
SM00279 HhH2, 1 hit
SM00482 POLAc, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF53098 SSF53098, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR00593 pola, 1 hit
PROSITEiView protein in PROSITE
PS00447 DNA_POLYMERASE_A, 1 hit

Sequencei

Sequence statusi: Complete.

O34996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERKKLVLV DGNSLAYRAF FALPLLSNDK GVHTNAVYGF AMILMKMLED
60 70 80 90 100
EKPTHMLVAF DAGKTTFRHG TFKEYKGGRQ KTPPELSEQM PFIRELLDAY
110 120 130 140 150
QISRYELEQY EADDIIGTLA KSAEKDGFEV KVFSGDKDLT QLATDKTTVA
160 170 180 190 200
ITRKGITDVE FYTPEHVKEK YGLTPEQIID MKGLMGDSSD NIPGVPGVGE
210 220 230 240 250
KTAIKLLKQF DSVEKLLESI DEVSGKKLKE KLEEFKDQAL MSKELATIMT
260 270 280 290 300
DAPIEVSVSG LEYQGFNREQ VIAIFKDLGF NTLLERLGED SAEAEQDQSL
310 320 330 340 350
EDINVKTVTD VTSDILVSPS AFVVEQIGDN YHEEPILGFS IVNETGAYFI
360 370 380 390 400
PKDIAVESEV FKEWVENDEQ KKWVFDSKRA VVALRWQGIE LKGAEFDTLL
410 420 430 440 450
AAYIINPGNS YDDVASVAKD YGLHIVSSDE SVYGKGAKRA VPSEDVLSEH
460 470 480 490 500
LGRKALAIQS LREKLVQELE NNDQLELFEE LEMPLALILG EMESTGVKVD
510 520 530 540 550
VDRLKRMGEE LGAKLKEYEE KIHEIAGEPF NINSPKQLGV ILFEKIGLPV
560 570 580 590 600
VKKTKTGYST SADVLEKLAD KHDIVDYILQ YRQIGKLQST YIEGLLKVTR
610 620 630 640 650
PDSHKVHTRF NQALTQTGRL SSTDPNLQNI PIRLEEGRKI RQAFVPSEKD
660 670 680 690 700
WLIFAADYSQ IELRVLAHIS KDENLIEAFT NDMDIHTKTA MDVFHVAKDE
710 720 730 740 750
VTSAMRRQAK AVNFGIVYGI SDYGLSQNLG ITRKEAGAFI DRYLESFQGV
760 770 780 790 800
KAYMEDSVQE AKQKGYVTTL MHRRRYIPEL TSRNFNIRSF AERTAMNTPI
810 820 830 840 850
QGSAADIIKK AMIDMAAKLK EKQLKARLLL QVHDELIFEA PKEEIEILEK
860 870 880
LVPEVMEHAL ALDVPLKVDF ASGPSWYDAK
Length:880
Mass (Da):99,092
Last modified:January 1, 1998 - v1
Checksum:iAFB434AFDF26F488
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA Translation: AAC00350.1
AL009126 Genomic DNA Translation: CAB14869.1
PIRiE69680
RefSeqiNP_390787.1, NC_000964.3
WP_004398870.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14869; CAB14869; BSU29090
GeneIDi936617
KEGGibsu:BSU29090
PATRICifig|224308.179.peg.3158

Similar proteinsi

Entry informationi

Entry nameiDPO1_BACSU
AccessioniPrimary (citable) accession number: O34996
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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