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Protein

DNA polymerase I

Gene

polA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU29090-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Ordered Locus Names:BSU29090
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012351 – 880DNA polymerase IAdd BLAST880

Proteomic databases

PaxDbiO34996.

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.

Protein-protein interaction databases

IntActiO34996. 8 interactors.
STRINGi224308.Bsubs1_010100015876.

Structurei

3D structure databases

ProteinModelPortaliO34996.
SMRiO34996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated
Contains 1 3'-5' exonuclease domain.Curated
Contains 1 5'-3' exonuclease domain.Curated

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.
HOGENOMiHOG000020998.
InParanoidiO34996.
KOiK02335.
OMAiCFDTETT.
PhylomeDBiO34996.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERKKLVLV DGNSLAYRAF FALPLLSNDK GVHTNAVYGF AMILMKMLED
60 70 80 90 100
EKPTHMLVAF DAGKTTFRHG TFKEYKGGRQ KTPPELSEQM PFIRELLDAY
110 120 130 140 150
QISRYELEQY EADDIIGTLA KSAEKDGFEV KVFSGDKDLT QLATDKTTVA
160 170 180 190 200
ITRKGITDVE FYTPEHVKEK YGLTPEQIID MKGLMGDSSD NIPGVPGVGE
210 220 230 240 250
KTAIKLLKQF DSVEKLLESI DEVSGKKLKE KLEEFKDQAL MSKELATIMT
260 270 280 290 300
DAPIEVSVSG LEYQGFNREQ VIAIFKDLGF NTLLERLGED SAEAEQDQSL
310 320 330 340 350
EDINVKTVTD VTSDILVSPS AFVVEQIGDN YHEEPILGFS IVNETGAYFI
360 370 380 390 400
PKDIAVESEV FKEWVENDEQ KKWVFDSKRA VVALRWQGIE LKGAEFDTLL
410 420 430 440 450
AAYIINPGNS YDDVASVAKD YGLHIVSSDE SVYGKGAKRA VPSEDVLSEH
460 470 480 490 500
LGRKALAIQS LREKLVQELE NNDQLELFEE LEMPLALILG EMESTGVKVD
510 520 530 540 550
VDRLKRMGEE LGAKLKEYEE KIHEIAGEPF NINSPKQLGV ILFEKIGLPV
560 570 580 590 600
VKKTKTGYST SADVLEKLAD KHDIVDYILQ YRQIGKLQST YIEGLLKVTR
610 620 630 640 650
PDSHKVHTRF NQALTQTGRL SSTDPNLQNI PIRLEEGRKI RQAFVPSEKD
660 670 680 690 700
WLIFAADYSQ IELRVLAHIS KDENLIEAFT NDMDIHTKTA MDVFHVAKDE
710 720 730 740 750
VTSAMRRQAK AVNFGIVYGI SDYGLSQNLG ITRKEAGAFI DRYLESFQGV
760 770 780 790 800
KAYMEDSVQE AKQKGYVTTL MHRRRYIPEL TSRNFNIRSF AERTAMNTPI
810 820 830 840 850
QGSAADIIKK AMIDMAAKLK EKQLKARLLL QVHDELIFEA PKEEIEILEK
860 870 880
LVPEVMEHAL ALDVPLKVDF ASGPSWYDAK
Length:880
Mass (Da):99,092
Last modified:January 1, 1998 - v1
Checksum:iAFB434AFDF26F488
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00350.1.
AL009126 Genomic DNA. Translation: CAB14869.1.
PIRiE69680.
RefSeqiNP_390787.1. NC_000964.3.
WP_004398870.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14869; CAB14869; BSU29090.
GeneIDi936617.
KEGGibsu:BSU29090.
PATRICi18977710. VBIBacSub10457_3043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00350.1.
AL009126 Genomic DNA. Translation: CAB14869.1.
PIRiE69680.
RefSeqiNP_390787.1. NC_000964.3.
WP_004398870.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO34996.
SMRiO34996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34996. 8 interactors.
STRINGi224308.Bsubs1_010100015876.

Proteomic databases

PaxDbiO34996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14869; CAB14869; BSU29090.
GeneIDi936617.
KEGGibsu:BSU29090.
PATRICi18977710. VBIBacSub10457_3043.

Phylogenomic databases

eggNOGiENOG4105C2M. Bacteria.
COG0258. LUCA.
COG0749. LUCA.
HOGENOMiHOG000020998.
InParanoidiO34996.
KOiK02335.
OMAiCFDTETT.
PhylomeDBiO34996.

Enzyme and pathway databases

BioCyciBSUB:BSU29090-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.50.1010. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR020046. 5-3_exonucl_a-hlix_arch_N.
IPR020045. 5-3_exonuclease_C.
IPR002421. 5-3_exonuclease_N.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF01367. 5_3_exonuc. 1 hit.
PF02739. 5_3_exonuc_N. 1 hit.
PF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO1_BACSU
AccessioniPrimary (citable) accession number: O34996
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.