Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Formyltetrahydrofolate deformylase

Gene

purU

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).UniRule annotation

Catalytic activityi

10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Formyltetrahydrofolate deformylase (purU)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism, Purine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU13110-MONOMER.
UniPathwayiUPA00074; UER00170.

Names & Taxonomyi

Protein namesi
Recommended name:
Formyltetrahydrofolate deformylaseUniRule annotation (EC:3.5.1.10UniRule annotation)
Alternative name(s):
Formyl-FH(4) hydrolaseUniRule annotation
Gene namesi
Name:purUUniRule annotation
Synonyms:ykkE
Ordered Locus Names:BSU13110
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003608201 – 300Formyltetrahydrofolate deformylaseAdd BLAST300

Proteomic databases

PaxDbiO34990.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007261.

Structurei

3D structure databases

ProteinModelPortaliO34990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 102ACTUniRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the PurU family.UniRule annotation
Contains 1 ACT domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIY. Bacteria.
COG0788. LUCA.
HOGENOMiHOG000033576.
InParanoidiO34990.
KOiK01433.
OMAiTLCEAMS.
PhylomeDBiO34990.

Family and domain databases

Gene3Di3.40.50.170. 1 hit.
HAMAPiMF_01927. PurU. 1 hit.
InterProiIPR002912. ACT_dom.
IPR002376. Formyl_transf_N.
IPR004810. PurU.
[Graphical view]
PANTHERiPTHR10520:SF7. PTHR10520:SF7. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
PRINTSiPR01575. FFH4HYDRLASE.
SUPFAMiSSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00655. PurU. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYMTQRLD EYRDGNEDKG RLLVSCPDQP GIVSAVSAFL FEHGANIIES
60 70 80 90 100
NQYTTDPEGG RFFLRIEFDC AGIREKKSSL QAAFASVAEK FDMTWSLTLA
110 120 130 140 150
SELKRVAIFV SKELHCLHEL IWEWQTGNLM AEIAVVISNH EEARELVERL
160 170 180 190 200
NIPFHYMKAN KDIRAEVEKK QLELLEQYDV DVIVLARYMQ ILTPDFVSAH
210 220 230 240 250
PNRIINIHHS FLPAFIGANP YKRAYERGVK LIGATSHYVT NDLDEGPIIE
260 270 280 290 300
QDIERVDHRD NAEALKNIGR TIERSVLARA VKWHLEDRVI VHENKTIVFN
Length:300
Mass (Da):34,477
Last modified:June 16, 2009 - v2
Checksum:i3D8545C6802990E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113E → N in CAA05590 (Ref. 1) Curated1
Sequence conflicti254E → K in CAA05590 (Ref. 1) Curated1
Sequence conflicti264A → T in CAA05590 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA. Translation: CAA05590.1.
AL009126 Genomic DNA. Translation: CAB13168.2.
PIRiC69857.
RefSeqiNP_389194.2. NC_000964.3.
WP_003245802.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13168; CAB13168; BSU13110.
GeneIDi939854.
KEGGibsu:BSU13110.
PATRICi18974379. VBIBacSub10457_1382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA. Translation: CAA05590.1.
AL009126 Genomic DNA. Translation: CAB13168.2.
PIRiC69857.
RefSeqiNP_389194.2. NC_000964.3.
WP_003245802.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO34990.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007261.

Proteomic databases

PaxDbiO34990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13168; CAB13168; BSU13110.
GeneIDi939854.
KEGGibsu:BSU13110.
PATRICi18974379. VBIBacSub10457_1382.

Phylogenomic databases

eggNOGiENOG4105CIY. Bacteria.
COG0788. LUCA.
HOGENOMiHOG000033576.
InParanoidiO34990.
KOiK01433.
OMAiTLCEAMS.
PhylomeDBiO34990.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00170.
BioCyciBSUB:BSU13110-MONOMER.

Family and domain databases

Gene3Di3.40.50.170. 1 hit.
HAMAPiMF_01927. PurU. 1 hit.
InterProiIPR002912. ACT_dom.
IPR002376. Formyl_transf_N.
IPR004810. PurU.
[Graphical view]
PANTHERiPTHR10520:SF7. PTHR10520:SF7. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
PRINTSiPR01575. FFH4HYDRLASE.
SUPFAMiSSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00655. PurU. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPURU_BACSU
AccessioniPrimary (citable) accession number: O34990
Secondary accession number(s): Q796M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.