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Protein

Formyltetrahydrofolate deformylase

Gene

purU

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).UniRule annotation

Catalytic activityi

10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Formyltetrahydrofolate deformylase (purU)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism, Purine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU13110-MONOMER
UniPathwayiUPA00074; UER00170

Names & Taxonomyi

Protein namesi
Recommended name:
Formyltetrahydrofolate deformylaseUniRule annotation (EC:3.5.1.10UniRule annotation)
Alternative name(s):
Formyl-FH(4) hydrolaseUniRule annotation
Gene namesi
Name:purUUniRule annotation
Synonyms:ykkE
Ordered Locus Names:BSU13110
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003608201 – 300Formyltetrahydrofolate deformylaseAdd BLAST300

Proteomic databases

PaxDbiO34990

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007261

Structurei

3D structure databases

ProteinModelPortaliO34990
SMRiO34990
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 102ACTUniRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the PurU family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIY Bacteria
COG0788 LUCA
HOGENOMiHOG000033576
InParanoidiO34990
KOiK01433
OMAiHADHHTD
PhylomeDBiO34990

Family and domain databases

HAMAPiMF_01927 PurU, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR002376 Formyl_transf_N
IPR036477 Formyl_transf_N_sf
IPR004810 PurU
PANTHERiPTHR42706 PTHR42706, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF00551 Formyl_trans_N, 1 hit
PRINTSiPR01575 FFH4HYDRLASE
SUPFAMiSSF53328 SSF53328, 1 hit
TIGRFAMsiTIGR00655 PurU, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit

Sequencei

Sequence statusi: Complete.

O34990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYMTQRLD EYRDGNEDKG RLLVSCPDQP GIVSAVSAFL FEHGANIIES
60 70 80 90 100
NQYTTDPEGG RFFLRIEFDC AGIREKKSSL QAAFASVAEK FDMTWSLTLA
110 120 130 140 150
SELKRVAIFV SKELHCLHEL IWEWQTGNLM AEIAVVISNH EEARELVERL
160 170 180 190 200
NIPFHYMKAN KDIRAEVEKK QLELLEQYDV DVIVLARYMQ ILTPDFVSAH
210 220 230 240 250
PNRIINIHHS FLPAFIGANP YKRAYERGVK LIGATSHYVT NDLDEGPIIE
260 270 280 290 300
QDIERVDHRD NAEALKNIGR TIERSVLARA VKWHLEDRVI VHENKTIVFN
Length:300
Mass (Da):34,477
Last modified:June 16, 2009 - v2
Checksum:i3D8545C6802990E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113E → N in CAA05590 (Ref. 1) Curated1
Sequence conflicti254E → K in CAA05590 (Ref. 1) Curated1
Sequence conflicti264A → T in CAA05590 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA Translation: CAA05590.1
AL009126 Genomic DNA Translation: CAB13168.2
PIRiC69857
RefSeqiNP_389194.2, NC_000964.3
WP_003245802.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13168; CAB13168; BSU13110
GeneIDi939854
KEGGibsu:BSU13110
PATRICifig|224308.179.peg.1423

Similar proteinsi

Entry informationi

Entry nameiPURU_BACSU
AccessioniPrimary (citable) accession number: O34990
Secondary accession number(s): Q796M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health