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Protein

High-affinity zinc uptake system binding-protein ZnuA

Gene

znuA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the high-affinity ABC transporter complex ZnuABC involved in zinc import (Probable). ZnuABC-mediated zinc transport is required for comF expression and competence development.Curated2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport, Zinc transport

Keywords - Ligandi

Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU02850-MONOMER.

Protein family/group databases

TCDBi3.A.1.15.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity zinc uptake system binding-protein ZnuA
Gene namesi
Name:znuA
Synonyms:adcA, ycdH
Ordered Locus Names:BSU02850
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene grow slowly in zinc-deficient medium; this effect is suppressed upon addition of exogenous Zn2+. Growth is slower yet in a double knockout with YciC. Disruption results in low transformability.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20PROSITE-ProRule annotationAdd BLAST20
ChainiPRO_000036080921 – 319High-affinity zinc uptake system binding-protein ZnuAAdd BLAST299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi21N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi21S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiO34966.

Expressioni

Inductioni

Repressed by zinc via the metallo-regulatory protein Zur.2 Publications

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ZnuC), two transmembrane proteins (ZnuB) and a solute-binding protein (ZnuA).Curated

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001573.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 43Combined sources7
Helixi44 – 54Combined sources11
Helixi55 – 57Combined sources3
Beta strandi58 – 64Combined sources7
Turni70 – 72Combined sources3
Helixi77 – 85Combined sources9
Beta strandi86 – 92Combined sources7
Turni94 – 96Combined sources3
Helixi100 – 105Combined sources6
Beta strandi109 – 111Combined sources3
Beta strandi113 – 116Combined sources4
Turni117 – 120Combined sources4
Helixi149 – 151Combined sources3
Helixi153 – 170Combined sources18
Helixi172 – 174Combined sources3
Helixi175 – 201Combined sources27
Beta strandi207 – 212Combined sources6
Helixi216 – 221Combined sources6
Beta strandi225 – 228Combined sources4
Beta strandi234 – 236Combined sources3
Helixi240 – 249Combined sources10
Beta strandi257 – 259Combined sources3
Helixi266 – 274Combined sources9
Beta strandi279 – 281Combined sources3
Helixi291 – 296Combined sources6
Helixi300 – 315Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O1EX-ray2.60A/B22-319[»]
ProteinModelPortaliO34966.
SMRiO34966.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34966.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DSE. Bacteria.
COG0803. LUCA.
HOGENOMiHOG000180308.
InParanoidiO34966.
KOiK09815.
OMAiSPHTYEP.
PhylomeDBiO34966.

Family and domain databases

InterProiIPR006129. AdhesinB.
IPR006128. Lipoprotein_4.
IPR006127. ZnuA-like.
[Graphical view]
PfamiPF01297. ZnuA. 1 hit.
[Graphical view]
PRINTSiPR00691. ADHESINB.
PR00690. ADHESNFAMILY.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O34966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKWSGLFV IAACFLLVAA CGNSSTKGSA DSKGDKLHVV TTFYPMYEFT
60 70 80 90 100
KQIVKDKGDV DLLIPSSVEP HDWEPTPKDI ANIQDADLFV YNSEYMETWV
110 120 130 140 150
PSAEKSMGQG HAVFVNASKG IDLMEGSEEE HEEHDHGEHE HSHAMDPHVW
160 170 180 190 200
LSPVLAQKEV KNITAQIVKQ DPDNKEYYEK NSKEYIAKLQ DLDKLYRTTA
210 220 230 240 250
KKAEKKEFIT QHTAFGYLAK EYGLKQVPIA GLSPDQEPSA ASLAKLKTYA
260 270 280 290 300
KEHNVKVIYF EEIASSKVAD TLASEIGAKT EVLNTLEGLS KEEQDKGLGY
310
IDIMKQNLDA LKDSLLVKS
Length:319
Mass (Da):35,660
Last modified:January 1, 1998 - v1
Checksum:iC66128D4FDE94D1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000617 Genomic DNA. Translation: BAA22246.1.
AL009126 Genomic DNA. Translation: CAB12079.1.
PIRiA69756.
RefSeqiNP_388167.1. NC_000964.3.
WP_003234733.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB12079; CAB12079; BSU02850.
GeneIDi938370.
KEGGibsu:BSU02850.
PATRICi18972129. VBIBacSub10457_0293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000617 Genomic DNA. Translation: BAA22246.1.
AL009126 Genomic DNA. Translation: CAB12079.1.
PIRiA69756.
RefSeqiNP_388167.1. NC_000964.3.
WP_003234733.1. NZ_JNCM01000030.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O1EX-ray2.60A/B22-319[»]
ProteinModelPortaliO34966.
SMRiO34966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001573.

Protein family/group databases

TCDBi3.A.1.15.11. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiO34966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12079; CAB12079; BSU02850.
GeneIDi938370.
KEGGibsu:BSU02850.
PATRICi18972129. VBIBacSub10457_0293.

Phylogenomic databases

eggNOGiENOG4105DSE. Bacteria.
COG0803. LUCA.
HOGENOMiHOG000180308.
InParanoidiO34966.
KOiK09815.
OMAiSPHTYEP.
PhylomeDBiO34966.

Enzyme and pathway databases

BioCyciBSUB:BSU02850-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO34966.

Family and domain databases

InterProiIPR006129. AdhesinB.
IPR006128. Lipoprotein_4.
IPR006127. ZnuA-like.
[Graphical view]
PfamiPF01297. ZnuA. 1 hit.
[Graphical view]
PRINTSiPR00691. ADHESINB.
PR00690. ADHESNFAMILY.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZNUA_BACSU
AccessioniPrimary (citable) accession number: O34966
Secondary accession number(s): Q797R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.