ID   MAO4_BACSU              Reviewed;         410 AA.
AC   O34962;
DT   28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Probable NAD-dependent malic enzyme 4;
DE            Short=NAD-ME 4;
DE            EC=1.1.1.38;
GN   Name=ytsJ; OrderedLocusNames=BSU29220;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98048467; PubMed=9387221;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes
RT   in the 200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; AF008220; AAC00339.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14882.1; -; Genomic_DNA.
DR   PIR; C70001; C70001.
DR   RefSeq; NP_390800.1; -.
DR   GeneID; 937378; -.
DR   GenomeReviews; AL009126_GR; BSU29220.
DR   KEGG; bsu:BSU29220; -.
DR   NMPDR; fig|224308.1.peg.2925; -.
DR   SubtiList; BG13922; ytsJ.
DR   HOGENOM; O34962; -.
DR   OMA; O34962; CNVPVFH.
DR   BioCyc; BSUB224308:BSU2918-MON; -.
DR   BRENDA; 1.1.1.38; 150.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    410       Probable NAD-dependent malic enzyme 4.
FT                                /FTId=PRO_0000160211.
FT   NP_BIND      73     80       NAD (By similarity).
FT   ACT_SITE     91     91       Proton acceptor (By similarity).
FT   METAL       133    133       Divalent metal cation (By similarity).
FT   METAL       134    134       Divalent metal cation (By similarity).
FT   BINDING     159    159       NAD (By similarity).
FT   BINDING     286    286       NAD (By similarity).
SQ   SEQUENCE   410 AA;  43667 MW;  3B9B2FDAAA349D61 CRC64;
     MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED INKVYDYTMK
     GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG VDAFPIALNT NDVDKIVETV
     KLLEPTFGGV NLEDIAAPNC FIIEERLKKE TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK
     SMSSIKVVAN GAGAAGIAII KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN
     QDRKDGSLKD VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG
     ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS LVSEDELSAD
     YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE KTRKLTIIGE
//