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O34962

- MAO4_BACSU

UniProt

O34962 - MAO4_BACSU

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Protein
Probable NAD-dependent malic enzyme 4
Gene
ytsJ, BSU29220
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Divalent metal cations. Prefers magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361Proton donor By similarity
Active sitei91 – 911Proton acceptor By similarity
Metal bindingi133 – 1331Divalent metal cation By similarity
Metal bindingi134 – 1341Divalent metal cation By similarity
Binding sitei159 – 1591NAD By similarity
Binding sitei286 – 2861NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 808NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. malate dehydrogenase (decarboxylating) (NAD+) activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU29220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NAD-dependent malic enzyme 4 (EC:1.1.1.38)
Short name:
NAD-ME 4
Gene namesi
Name:ytsJ
Ordered Locus Names:BSU29220
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU29220.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Probable NAD-dependent malic enzyme 4
PRO_0000160211Add
BLAST

Proteomic databases

PaxDbiO34962.

Interactioni

Protein-protein interaction databases

IntActiO34962. 2 interactions.
MINTiMINT-8365494.
STRINGi224308.BSU29220.

Structurei

3D structure databases

ProteinModelPortaliO34962.
SMRiO34962. Positions 2-384.

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.

Phylogenomic databases

eggNOGiCOG0281.
HOGENOMiHOG000132447.
KOiK00027.
OMAiIMPEDAR.
OrthoDBiEOG6QCD9W.
PhylomeDBiO34962.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34962-1 [UniParc]FASTAAdd to Basket

« Hide

MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED    50
INKVYDYTMK GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG 100
VDAFPIALNT NDVDKIVETV KLLEPTFGGV NLEDIAAPNC FIIEERLKKE 150
TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK SMSSIKVVAN GAGAAGIAII 200
KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN QDRKDGSLKD 250
VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG 300
ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS 350
LVSEDELSAD YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE 400
KTRKLTIIGE 410
Length:410
Mass (Da):43,667
Last modified:January 1, 1998 - v1
Checksum:i3B9B2FDAAA349D61
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008220 Genomic DNA. Translation: AAC00339.1.
AL009126 Genomic DNA. Translation: CAB14882.1.
PIRiC70001.
RefSeqiNP_390800.1. NC_000964.3.
WP_003229415.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB14882; CAB14882; BSU29220.
GeneIDi937378.
KEGGibsu:BSU29220.
PATRICi18977738. VBIBacSub10457_3057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008220 Genomic DNA. Translation: AAC00339.1 .
AL009126 Genomic DNA. Translation: CAB14882.1 .
PIRi C70001.
RefSeqi NP_390800.1. NC_000964.3.
WP_003229415.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali O34962.
SMRi O34962. Positions 2-384.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O34962. 2 interactions.
MINTi MINT-8365494.
STRINGi 224308.BSU29220.

Proteomic databases

PaxDbi O34962.

Protocols and materials databases

DNASUi 937378.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14882 ; CAB14882 ; BSU29220 .
GeneIDi 937378.
KEGGi bsu:BSU29220.
PATRICi 18977738. VBIBacSub10457_3057.

Organism-specific databases

GenoListi BSU29220.

Phylogenomic databases

eggNOGi COG0281.
HOGENOMi HOG000132447.
KOi K00027.
OMAi IMPEDAR.
OrthoDBi EOG6QCD9W.
PhylomeDBi O34962.

Enzyme and pathway databases

BioCyci BSUB:BSU29220-MONOMER.

Family and domain databases

Gene3Di 3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view ]
PIRSFi PIRSF000106. ME. 1 hit.
PRINTSi PR00072. MALOXRDTASE.
SMARTi SM00919. Malic_M. 1 hit.
[Graphical view ]
PROSITEi PS00331. MALIC_ENZYMES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiMAO4_BACSU
AccessioniPrimary (citable) accession number: O34962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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