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Protein

Probable NAD-dependent malic enzyme 4

Gene

ytsJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36Proton donorBy similarity1
Active sitei91Proton acceptorBy similarity1
Metal bindingi133Divalent metal cationBy similarity1
Metal bindingi134Divalent metal cationBy similarity1
Binding sitei159NADBy similarity1
Binding sitei286NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 80NADBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU29220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NAD-dependent malic enzyme 4 (EC:1.1.1.38)
Short name:
NAD-ME 4
Gene namesi
Name:ytsJ
Ordered Locus Names:BSU29220
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602111 – 410Probable NAD-dependent malic enzyme 4Add BLAST410

Proteomic databases

PaxDbiO34962.
PRIDEiO34962.

Interactioni

Protein-protein interaction databases

IntActiO34962. 2 interactors.
MINTiMINT-8365494.
STRINGi224308.Bsubs1_010100015941.

Structurei

3D structure databases

ProteinModelPortaliO34962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C6K. Bacteria.
COG0281. LUCA.
HOGENOMiHOG000132447.
InParanoidiO34962.
KOiK00027.
OMAiVDAWPVV.
PhylomeDBiO34962.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED
60 70 80 90 100
INKVYDYTMK GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG
110 120 130 140 150
VDAFPIALNT NDVDKIVETV KLLEPTFGGV NLEDIAAPNC FIIEERLKKE
160 170 180 190 200
TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK SMSSIKVVAN GAGAAGIAII
210 220 230 240 250
KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN QDRKDGSLKD
260 270 280 290 300
VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG
310 320 330 340 350
ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS
360 370 380 390 400
LVSEDELSAD YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE
410
KTRKLTIIGE
Length:410
Mass (Da):43,667
Last modified:January 1, 1998 - v1
Checksum:i3B9B2FDAAA349D61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00339.1.
AL009126 Genomic DNA. Translation: CAB14882.1.
PIRiC70001.
RefSeqiNP_390800.1. NC_000964.3.
WP_003229415.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14882; CAB14882; BSU29220.
GeneIDi937378.
KEGGibsu:BSU29220.
PATRICi18977738. VBIBacSub10457_3057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00339.1.
AL009126 Genomic DNA. Translation: CAB14882.1.
PIRiC70001.
RefSeqiNP_390800.1. NC_000964.3.
WP_003229415.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO34962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34962. 2 interactors.
MINTiMINT-8365494.
STRINGi224308.Bsubs1_010100015941.

Proteomic databases

PaxDbiO34962.
PRIDEiO34962.

Protocols and materials databases

DNASUi937378.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14882; CAB14882; BSU29220.
GeneIDi937378.
KEGGibsu:BSU29220.
PATRICi18977738. VBIBacSub10457_3057.

Phylogenomic databases

eggNOGiENOG4105C6K. Bacteria.
COG0281. LUCA.
HOGENOMiHOG000132447.
InParanoidiO34962.
KOiK00027.
OMAiVDAWPVV.
PhylomeDBiO34962.

Enzyme and pathway databases

BioCyciBSUB:BSU29220-MONOMER.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAO4_BACSU
AccessioniPrimary (citable) accession number: O34962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.