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O34962

- MAO4_BACSU

UniProt

O34962 - MAO4_BACSU

Protein

Probable NAD-dependent malic enzyme 4

Gene

ytsJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = pyruvate + CO2 + NADH.
    Oxaloacetate = pyruvate + CO2.

    Cofactori

    Divalent metal cations. Prefers magnesium or manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei36 – 361Proton donorBy similarity
    Active sitei91 – 911Proton acceptorBy similarity
    Metal bindingi133 – 1331Divalent metal cationBy similarity
    Metal bindingi134 – 1341Divalent metal cationBy similarity
    Binding sitei159 – 1591NADBy similarity
    Binding sitei286 – 2861NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 808NADBy similarity

    GO - Molecular functioni

    1. malate dehydrogenase (decarboxylating) (NAD+) activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. NAD binding Source: InterPro
    4. oxaloacetate decarboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. malate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciBSUB:BSU29220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable NAD-dependent malic enzyme 4 (EC:1.1.1.38)
    Short name:
    NAD-ME 4
    Gene namesi
    Name:ytsJ
    Ordered Locus Names:BSU29220
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU29220.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Probable NAD-dependent malic enzyme 4PRO_0000160211Add
    BLAST

    Proteomic databases

    PaxDbiO34962.

    Interactioni

    Protein-protein interaction databases

    IntActiO34962. 2 interactions.
    MINTiMINT-8365494.
    STRINGi224308.BSU29220.

    Structurei

    3D structure databases

    ProteinModelPortaliO34962.
    SMRiO34962. Positions 2-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malic enzymes family.Curated

    Phylogenomic databases

    eggNOGiCOG0281.
    HOGENOMiHOG000132447.
    KOiK00027.
    OMAiIMPEDAR.
    OrthoDBiEOG6QCD9W.
    PhylomeDBiO34962.

    Family and domain databases

    Gene3Di3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000106. ME. 1 hit.
    PRINTSiPR00072. MALOXRDTASE.
    SMARTiSM00919. Malic_M. 1 hit.
    [Graphical view]
    PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O34962-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED    50
    INKVYDYTMK GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG 100
    VDAFPIALNT NDVDKIVETV KLLEPTFGGV NLEDIAAPNC FIIEERLKKE 150
    TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK SMSSIKVVAN GAGAAGIAII 200
    KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN QDRKDGSLKD 250
    VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG 300
    ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS 350
    LVSEDELSAD YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE 400
    KTRKLTIIGE 410
    Length:410
    Mass (Da):43,667
    Last modified:January 1, 1998 - v1
    Checksum:i3B9B2FDAAA349D61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008220 Genomic DNA. Translation: AAC00339.1.
    AL009126 Genomic DNA. Translation: CAB14882.1.
    PIRiC70001.
    RefSeqiNP_390800.1. NC_000964.3.
    WP_003229415.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14882; CAB14882; BSU29220.
    GeneIDi937378.
    KEGGibsu:BSU29220.
    PATRICi18977738. VBIBacSub10457_3057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008220 Genomic DNA. Translation: AAC00339.1 .
    AL009126 Genomic DNA. Translation: CAB14882.1 .
    PIRi C70001.
    RefSeqi NP_390800.1. NC_000964.3.
    WP_003229415.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali O34962.
    SMRi O34962. Positions 2-384.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O34962. 2 interactions.
    MINTi MINT-8365494.
    STRINGi 224308.BSU29220.

    Proteomic databases

    PaxDbi O34962.

    Protocols and materials databases

    DNASUi 937378.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14882 ; CAB14882 ; BSU29220 .
    GeneIDi 937378.
    KEGGi bsu:BSU29220.
    PATRICi 18977738. VBIBacSub10457_3057.

    Organism-specific databases

    GenoListi BSU29220.

    Phylogenomic databases

    eggNOGi COG0281.
    HOGENOMi HOG000132447.
    KOi K00027.
    OMAi IMPEDAR.
    OrthoDBi EOG6QCD9W.
    PhylomeDBi O34962.

    Enzyme and pathway databases

    BioCyci BSUB:BSU29220-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000106. ME. 1 hit.
    PRINTSi PR00072. MALOXRDTASE.
    SMARTi SM00919. Malic_M. 1 hit.
    [Graphical view ]
    PROSITEi PS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
      Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
      Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiMAO4_BACSU
    AccessioniPrimary (citable) accession number: O34962
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3