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O34962 (MAO4_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable NAD-dependent malic enzyme 4

Short name=NAD-ME 4
EC=1.1.1.38
Gene names
Name:ytsJ
Ordered Locus Names:BSU29220
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH.

Oxaloacetate = pyruvate + CO2.

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Sequence similarities

Belongs to the malic enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Probable NAD-dependent malic enzyme 4
PRO_0000160211

Regions

Nucleotide binding73 – 808NAD By similarity

Sites

Active site361Proton donor By similarity
Active site911Proton acceptor By similarity
Metal binding1331Divalent metal cation By similarity
Metal binding1341Divalent metal cation By similarity
Binding site1591NAD By similarity
Binding site2861NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
O34962 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 3B9B2FDAAA349D61

FASTA41043,667
        10         20         30         40         50         60 
MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED INKVYDYTMK 

        70         80         90        100        110        120 
GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG VDAFPIALNT NDVDKIVETV 

       130        140        150        160        170        180 
KLLEPTFGGV NLEDIAAPNC FIIEERLKKE TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK 

       190        200        210        220        230        240 
SMSSIKVVAN GAGAAGIAII KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN 

       250        260        270        280        290        300 
QDRKDGSLKD VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG 

       310        320        330        340        350        360 
ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS LVSEDELSAD 

       370        380        390        400        410 
YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE KTRKLTIIGE 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF008220 Genomic DNA. Translation: AAC00339.1.
AL009126 Genomic DNA. Translation: CAB14882.1.
PIRC70001.
RefSeqNP_390800.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34962.
SMRO34962. Positions 2-384.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO34962. 2 interactions.
MINTMINT-8365494.
STRING224308.BSU29220.

Proteomic databases

PaxDbO34962.

Protocols and materials databases

DNASU937378.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14882; CAB14882; BSU29220.
GeneID937378.
KEGGbsu:BSU29220.
PATRIC18977738. VBIBacSub10457_3057.

Organism-specific databases

GenoListBSU29220.

Phylogenomic databases

eggNOGCOG0281.
HOGENOMHOG000132447.
KOK00027.
OMAFTNQDRK.
OrthoDBEOG6QCD9W.
ProtClustDBCLSK2460949.

Enzyme and pathway databases

BioCycBSUB:BSU29220-MONOMER.

Family and domain databases

Gene3D3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAO4_BACSU
AccessionPrimary (citable) accession number: O34962
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 1, 1998
Last modified: January 22, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList