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Protein

Probable BsuMI modification methylase subunit YdiO

Gene

ydiO

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This methylase may recognize the double-stranded sequence CTCGAG, causing specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsuMI endonuclease.2 Publications

Caution

The characterized enzyme was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei176PROSITE-ProRule annotation1

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  • methyltransferase activity Source: UniProtKB

GO - Biological processi

  • DNA methylation on cytosine Source: UniProtKB
  • DNA restriction-modification system Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU06060-MONOMER

Protein family/group databases

REBASEi3612 M1.BsuMI

Names & Taxonomyi

Protein namesi
Recommended name:
Probable BsuMI modification methylase subunit YdiO (EC:2.1.1.37)
Short name:
M1.BsuMI
Alternative name(s):
Cytosine-specific methyltransferase M1.BsuMI
Gene namesi
Name:ydiO
Ordered Locus Names:BSU06060
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025) has been disrupted.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003798841 – 427Probable BsuMI modification methylase subunit YdiOAdd BLAST427

Proteomic databases

PaxDbiO34939
PRIDEiO34939

Expressioni

Developmental stagei

Not expressed during sporulation.1 Publication

Inductioni

Constitutively expressed during exponential growth. Encoded in an operon with ydiP and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible.1 Publication

Interactioni

Subunit structurei

May form a complex with YdiP, also seems to be active alone.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003403

Structurei

3D structure databases

ProteinModelPortaliO34939
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 427SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST344

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DVF Bacteria
COG0270 LUCA
HOGENOMiHOG000098493
InParanoidiO34939
KOiK00558
OMAiHELYDES
PhylomeDBiO34939

Family and domain databases

InterProiView protein in InterPro
IPR001525 C5_MeTfrase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

O34939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNFILNENK QLSLAIEDEN IENFYIDGTD LVRKIIRRSG SGVTSRVPVL
60 70 80 90 100
STQDLENKNL HELYDESWLR MKNRPNTELT TESINIADLF SGCGGLSLGV
110 120 130 140 150
WEACRALGIN PRFSFACDLN EAALSVYEKN FSPDFSLNES IEKHINGELG
160 170 180 190 200
APLTVEEQRI KDKVKKIDFI LAGPPCQGHS DLNNHTRRKD PRNALLMRVS
210 220 230 240 250
RVIELFQPSS VLVENVPGII HDKSGSFKEF KNHLKTQGYY FDEIVLNAEK
260 270 280 290 300
LGVSQARRRY FIFASKTPVS SLNQINEFYS TNSRPISWAI SDLVENVGDD
310 320 330 340 350
IFNTASEHSL ENKRRIEYLF ENNLFELPNS ERPDCHRLKP HSYKSVYGRM
360 370 380 390 400
YWDRPAPTIT RGFGSTGQGR FVHSLLKRTI TPHEAARIQF FPDFFNFGDL
410 420
RRRQYQDVIG NAVPSKLSYL LALHQLR
Length:427
Mass (Da):48,909
Last modified:January 1, 1998 - v1
Checksum:i3B02F48DD9CF1E30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007637 Genomic DNA Translation: BAA22750.1
AL009126 Genomic DNA Translation: CAB12425.1
PIRiH69787
RefSeqiNP_388487.1, NC_000964.3
WP_003234063.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12425; CAB12425; BSU06060
GeneIDi939892
KEGGibsu:BSU06060
PATRICifig|224308.179.peg.655

Similar proteinsi

Entry informationi

Entry nameiYDIO_BACSU
AccessioniPrimary (citable) accession number: O34939
Secondary accession number(s): Q797D1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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