Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O34939 (YDIO_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable BsuMI modification methylase subunit YdiO

Short name=M1.BsuMI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase M1.BsuMI
Gene names
Name:ydiO
Ordered Locus Names:BSU06060
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This methylase may recognize the double-stranded sequence CTCGAG, causing specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsuMI endonuclease. Ref.3 Ref.5

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subunit structure

May form a complex with YdiP, also seems to be active alone.

Developmental stage

Not expressed during sporulation. Ref.5

Induction

Constitutively expressed during exponential growth. Encoded in an operon with ydiP and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible. Ref.5

Disruption phenotype

Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025) has been disrupted. Ref.5

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Caution

The characterized enzyme (Ref.3) was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Probable BsuMI modification methylase subunit YdiO
PRO_0000379884

Regions

Domain84 – 427344SAM-dependent MTase C5-type

Sites

Active site1761 By similarity

Sequences

Sequence LengthMass (Da)Tools
O34939 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 3B02F48DD9CF1E30

FASTA42748,909
        10         20         30         40         50         60 
MTNFILNENK QLSLAIEDEN IENFYIDGTD LVRKIIRRSG SGVTSRVPVL STQDLENKNL 

        70         80         90        100        110        120 
HELYDESWLR MKNRPNTELT TESINIADLF SGCGGLSLGV WEACRALGIN PRFSFACDLN 

       130        140        150        160        170        180 
EAALSVYEKN FSPDFSLNES IEKHINGELG APLTVEEQRI KDKVKKIDFI LAGPPCQGHS 

       190        200        210        220        230        240 
DLNNHTRRKD PRNALLMRVS RVIELFQPSS VLVENVPGII HDKSGSFKEF KNHLKTQGYY 

       250        260        270        280        290        300 
FDEIVLNAEK LGVSQARRRY FIFASKTPVS SLNQINEFYS TNSRPISWAI SDLVENVGDD 

       310        320        330        340        350        360 
IFNTASEHSL ENKRRIEYLF ENNLFELPNS ERPDCHRLKP HSYKSVYGRM YWDRPAPTIT 

       370        380        390        400        410        420 
RGFGSTGQGR FVHSLLKRTI TPHEAARIQF FPDFFNFGDL RRRQYQDVIG NAVPSKLSYL 


LALHQLR 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"DNA methyltransferase of Bacillus subtilis Marburg: purification, properties and further evidence of specificity."
Guha S.
Gene 74:77-81(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
Bron S., Janniere L., Ehrlich S.D.
Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA TARGET SEQUENCE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[5]"Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
Ohshima H., Matsuoka S., Asai K., Sadaie Y.
J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007637 Genomic DNA. Translation: BAA22750.1.
AL009126 Genomic DNA. Translation: CAB12425.1.
PIRH69787.
RefSeqNP_388487.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34939.
SMRO34939. Positions 84-427.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU06060.

Protein family/group databases

REBASE3612. M1.BsuMI.

Proteomic databases

PaxDbO34939.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12425; CAB12425; BSU06060.
GeneID939892.
KEGGbsu:BSU06060.
PATRIC18972844. VBIBacSub10457_0636.

Organism-specific databases

GenoListBSU06060.

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000098493.
KOK00558.
OMAFSAGLEC.
OrthoDBEOG6KMB5M.
PhylomeDBO34939.

Enzyme and pathway databases

BioCycBSUB:BSU06060-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYDIO_BACSU
AccessionPrimary (citable) accession number: O34939
Secondary accession number(s): Q797D1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList