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Protein

NAD kinase 2

Gene

nadK2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521Proton acceptorUniRule annotation
Binding sitei151 – 1511NADUniRule annotation
Binding sitei153 – 1531NADUniRule annotation
Binding sitei188 – 1881NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi52 – 532NADUniRule annotation
Nucleotide bindingi124 – 1252NADUniRule annotation
Nucleotide bindingi164 – 1696NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU29540-MONOMER.
BRENDAi2.7.1.23. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 2UniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinase 2UniRule annotation
Gene namesi
Name:nadK2UniRule annotation
Ordered Locus Names:BSU29540
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU29540. [Micado]

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267NAD kinase 2PRO_0000120599Add
BLAST

Proteomic databases

PaxDbiO34934.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU29540.

Structurei

3D structure databases

ProteinModelPortaliO34934.
SMRiO34934. Positions 5-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
InParanoidiO34934.
KOiK00858.
OMAiRRNVYFF.
OrthoDBiEOG6PZXDR.
PhylomeDBiO34934.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

O34934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQRRNVYF FHKQDQETNE QARSLTQLAE EHGFTVVNQH SDANIIASIG
60 70 80 90 100
GDGTFLQAVR KTNFRDDCLY VGITKKGKAH LYCDFHSDER EKMVDAMTFE
110 120 130 140 150
QIEVRKYPLI EVTVDQASPF HCLNEVSIRS SIIKTFVMDV LIDDLHFETF
160 170 180 190 200
RGDGMIISTP TGSTAYNKSV AGAVVDPLLP CMQVSELASL NNNTYRTLGS
210 220 230 240 250
PFVLSSDRKL TLRVVQDGNE HPIIGLDNEA LSTRNVKTIE IKLSNKKIKT
260
VKLKDNSFWE KVKRTFL
Length:267
Mass (Da):30,253
Last modified:January 1, 1998 - v1
Checksum:i174092CC7202129B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00401.1.
AL009126 Genomic DNA. Translation: CAB14932.1.
PIRiB69990.
RefSeqiNP_390832.1. NC_000964.3.
WP_004399091.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14932; CAB14932; BSU29540.
GeneIDi936767.
KEGGibsu:BSU29540.
PATRICi18977812. VBIBacSub10457_3094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00401.1.
AL009126 Genomic DNA. Translation: CAB14932.1.
PIRiB69990.
RefSeqiNP_390832.1. NC_000964.3.
WP_004399091.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO34934.
SMRiO34934. Positions 5-267.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU29540.

Proteomic databases

PaxDbiO34934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14932; CAB14932; BSU29540.
GeneIDi936767.
KEGGibsu:BSU29540.
PATRICi18977812. VBIBacSub10457_3094.

Organism-specific databases

GenoListiBSU29540. [Micado]

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
InParanoidiO34934.
KOiK00858.
OMAiRRNVYFF.
OrthoDBiEOG6PZXDR.
PhylomeDBiO34934.

Enzyme and pathway databases

BioCyciBSUB:BSU29540-MONOMER.
BRENDAi2.7.1.23. 658.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiNADK2_BACSU
AccessioniPrimary (citable) accession number: O34934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: January 1, 1998
Last modified: April 29, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.