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Protein

Pulcherriminic acid synthase

Gene

cypX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. Catalyzes the oxidation of cyclo(L-Leu-L-Leu) (cLL) to yield pulcherriminic acid which forms pulcherrimin via a nonenzymic reaction with Fe3+. Substrates with small alkyl groups (cAA, cLG, cLP) exhibit weaker binding to CYP134A1, but substrates with larger hydrophobic side chains bind in a similar regime to cLL.1 Publication

Catalytic activityi

Cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O.1 Publication

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62Heme1 Publication1
Binding sitei229Heme1 Publication1
Binding sitei285Heme1 Publication1
Metal bindingi353Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

  • pigment biosynthetic process Source: UniProtKB
  • sterol metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU35060-MONOMER.
MetaCyc:BSU35060-MONOMER.
BRENDAi1.14.15.13. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulcherriminic acid synthase (EC:1.14.15.13)
Alternative name(s):
CYP134A1
Cyclo-L-leucyl-L-leucyl dipeptide oxidase
Cytochrome P450 CypX
Gene namesi
Name:cypX
Synonyms:cyp134, cypB
Ordered Locus Names:BSU35060
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000522341 – 405Pulcherriminic acid synthaseAdd BLAST405

Proteomic databases

PaxDbiO34926.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018976.

Structurei

Secondary structure

1405
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 17Combined sources5
Helixi19 – 21Combined sources3
Helixi23 – 29Combined sources7
Beta strandi31 – 35Combined sources5
Turni36 – 39Combined sources4
Beta strandi40 – 43Combined sources4
Helixi46 – 54Combined sources9
Turni56 – 58Combined sources3
Beta strandi64 – 66Combined sources3
Turni69 – 71Combined sources3
Helixi89 – 98Combined sources10
Helixi100 – 116Combined sources17
Turni117 – 123Combined sources7
Beta strandi124 – 126Combined sources3
Helixi127 – 131Combined sources5
Helixi132 – 143Combined sources12
Helixi148 – 150Combined sources3
Helixi151 – 166Combined sources16
Helixi172 – 196Combined sources25
Helixi204 – 208Combined sources5
Helixi220 – 250Combined sources31
Helixi253 – 261Combined sources9
Helixi263 – 265Combined sources3
Helixi266 – 276Combined sources11
Beta strandi282 – 289Combined sources8
Beta strandi291 – 295Combined sources5
Beta strandi296 – 298Combined sources3
Beta strandi303 – 307Combined sources5
Helixi308 – 312Combined sources5
Turni315 – 317Combined sources3
Beta strandi318 – 320Combined sources3
Helixi333 – 335Combined sources3
Beta strandi338 – 340Combined sources3
Helixi349 – 351Combined sources3
Helixi356 – 373Combined sources18
Beta strandi390 – 392Combined sources3
Beta strandi394 – 396Combined sources3
Beta strandi399 – 401Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NC3X-ray2.66A/B1-405[»]
3NC5X-ray2.90A/B1-405[»]
3NC6X-ray3.10A/B1-405[»]
3NC7X-ray3.30A/B1-405[»]
ProteinModelPortaliO34926.
SMRiO34926.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34926.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiENOG4106A3M. Bacteria.
COG2124. LUCA.
HOGENOMiHOG000243678.
InParanoidiO34926.
KOiK17474.
OMAiCTSEYEG.
PhylomeDBiO34926.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030904. CypX.
IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
TIGRFAMsiTIGR04538. P450_cycloAA_1. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34926-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSIKLFSV LSDQFQNNPY AYFSQLREED PVHYEESIDS YFISRYHDVR
60 70 80 90 100
YILQHPDIFT TKSLVERAEP VMRGPVLAQM HGKEHSAKRR IVVRSFIGDA
110 120 130 140 150
LDHLSPLIKQ NAENLLAPYL ERGKSDLVND FGKTFAVCVT MDMLGLDKRD
160 170 180 190 200
HEKISEWHSG VADFITSISQ SPEARAHSLW CSEQLSQYLM PVIKERRVNP
210 220 230 240 250
GSDLISILCT SEYEGMALSD KDILALILNV LLAATEPADK TLALMIYHLL
260 270 280 290 300
NNPEQMNDVL ADRSLVPRAI AETLRYKPPV QLIPRQLSQD TVVGGMEIKK
310 320 330 340 350
DTIVFCMIGA ANRDPEAFEQ PDVFNIHRED LGIKSAFSGA ARHLAFGSGI
360 370 380 390 400
HNCVGAAFAK NEIEIVANIV LDKMRNIRLE EDFCYAESGL YTRGPVSLLV

AFDGA
Length:405
Mass (Da):45,473
Last modified:January 1, 1998 - v1
Checksum:iA943DDFEAB67BD01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67280.1.
AL009126 Genomic DNA. Translation: CAB15511.1.
PIRiF69611.
RefSeqiNP_391386.1. NC_000964.3.
WP_003244436.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15511; CAB15511; BSU35060.
GeneIDi936624.
KEGGibsu:BSU35060.
PATRICi18979018. VBIBacSub10457_3671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67280.1.
AL009126 Genomic DNA. Translation: CAB15511.1.
PIRiF69611.
RefSeqiNP_391386.1. NC_000964.3.
WP_003244436.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NC3X-ray2.66A/B1-405[»]
3NC5X-ray2.90A/B1-405[»]
3NC6X-ray3.10A/B1-405[»]
3NC7X-ray3.30A/B1-405[»]
ProteinModelPortaliO34926.
SMRiO34926.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018976.

Proteomic databases

PaxDbiO34926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15511; CAB15511; BSU35060.
GeneIDi936624.
KEGGibsu:BSU35060.
PATRICi18979018. VBIBacSub10457_3671.

Phylogenomic databases

eggNOGiENOG4106A3M. Bacteria.
COG2124. LUCA.
HOGENOMiHOG000243678.
InParanoidiO34926.
KOiK17474.
OMAiCTSEYEG.
PhylomeDBiO34926.

Enzyme and pathway databases

BioCyciBSUB:BSU35060-MONOMER.
MetaCyc:BSU35060-MONOMER.
BRENDAi1.14.15.13. 658.

Miscellaneous databases

EvolutionaryTraceiO34926.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030904. CypX.
IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
TIGRFAMsiTIGR04538. P450_cycloAA_1. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYPX_BACSU
AccessioniPrimary (citable) accession number: O34926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.