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Protein

Pulcherriminic acid synthase

Gene

cypX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. Catalyzes the oxidation of cyclo(L-Leu-L-Leu) (cLL) to yield pulcherriminic acid which forms pulcherrimin via a nonenzymic reaction with Fe3+. Substrates with small alkyl groups (cAA, cLG, cLP) exhibit weaker binding to CYP134A1, but substrates with larger hydrophobic side chains bind in a similar regime to cLL.1 Publication

Catalytic activityi

Cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O.1 Publication

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621Heme1 Publication
Binding sitei229 – 2291Heme1 Publication
Binding sitei285 – 2851Heme1 Publication
Metal bindingi353 – 3531Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

  • pigment biosynthetic process Source: UniProtKB
  • sterol metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU35060-MONOMER.
MetaCyc:BSU35060-MONOMER.
BRENDAi1.14.15.13. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulcherriminic acid synthase (EC:1.14.15.13)
Alternative name(s):
CYP134A1
Cyclo-L-leucyl-L-leucyl dipeptide oxidase
Cytochrome P450 CypX
Gene namesi
Name:cypX
Synonyms:cyp134, cypB
Ordered Locus Names:BSU35060
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Pulcherriminic acid synthasePRO_0000052234Add
BLAST

Proteomic databases

PaxDbiO34926.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018976.

Structurei

Secondary structure

1
405
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 175Combined sources
Helixi19 – 213Combined sources
Helixi23 – 297Combined sources
Beta strandi31 – 355Combined sources
Turni36 – 394Combined sources
Beta strandi40 – 434Combined sources
Helixi46 – 549Combined sources
Turni56 – 583Combined sources
Beta strandi64 – 663Combined sources
Turni69 – 713Combined sources
Helixi89 – 9810Combined sources
Helixi100 – 11617Combined sources
Turni117 – 1237Combined sources
Beta strandi124 – 1263Combined sources
Helixi127 – 1315Combined sources
Helixi132 – 14312Combined sources
Helixi148 – 1503Combined sources
Helixi151 – 16616Combined sources
Helixi172 – 19625Combined sources
Helixi204 – 2085Combined sources
Helixi220 – 25031Combined sources
Helixi253 – 2619Combined sources
Helixi263 – 2653Combined sources
Helixi266 – 27611Combined sources
Beta strandi282 – 2898Combined sources
Beta strandi291 – 2955Combined sources
Beta strandi296 – 2983Combined sources
Beta strandi303 – 3075Combined sources
Helixi308 – 3125Combined sources
Turni315 – 3173Combined sources
Beta strandi318 – 3203Combined sources
Helixi333 – 3353Combined sources
Beta strandi338 – 3403Combined sources
Helixi349 – 3513Combined sources
Helixi356 – 37318Combined sources
Beta strandi390 – 3923Combined sources
Beta strandi394 – 3963Combined sources
Beta strandi399 – 4013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NC3X-ray2.66A/B1-405[»]
3NC5X-ray2.90A/B1-405[»]
3NC6X-ray3.10A/B1-405[»]
3NC7X-ray3.30A/B1-405[»]
ProteinModelPortaliO34926.
SMRiO34926. Positions 4-404.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34926.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiENOG4106A3M. Bacteria.
COG2124. LUCA.
HOGENOMiHOG000243678.
InParanoidiO34926.
KOiK17474.
OMAiCTSEYEG.
PhylomeDBiO34926.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030904. CypX.
IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
TIGRFAMsiTIGR04538. P450_cycloAA_1. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34926-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSIKLFSV LSDQFQNNPY AYFSQLREED PVHYEESIDS YFISRYHDVR
60 70 80 90 100
YILQHPDIFT TKSLVERAEP VMRGPVLAQM HGKEHSAKRR IVVRSFIGDA
110 120 130 140 150
LDHLSPLIKQ NAENLLAPYL ERGKSDLVND FGKTFAVCVT MDMLGLDKRD
160 170 180 190 200
HEKISEWHSG VADFITSISQ SPEARAHSLW CSEQLSQYLM PVIKERRVNP
210 220 230 240 250
GSDLISILCT SEYEGMALSD KDILALILNV LLAATEPADK TLALMIYHLL
260 270 280 290 300
NNPEQMNDVL ADRSLVPRAI AETLRYKPPV QLIPRQLSQD TVVGGMEIKK
310 320 330 340 350
DTIVFCMIGA ANRDPEAFEQ PDVFNIHRED LGIKSAFSGA ARHLAFGSGI
360 370 380 390 400
HNCVGAAFAK NEIEIVANIV LDKMRNIRLE EDFCYAESGL YTRGPVSLLV

AFDGA
Length:405
Mass (Da):45,473
Last modified:January 1, 1998 - v1
Checksum:iA943DDFEAB67BD01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67280.1.
AL009126 Genomic DNA. Translation: CAB15511.1.
PIRiF69611.
RefSeqiNP_391386.1. NC_000964.3.
WP_003244436.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15511; CAB15511; BSU35060.
GeneIDi936624.
KEGGibsu:BSU35060.
PATRICi18979018. VBIBacSub10457_3671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA. Translation: AAC67280.1.
AL009126 Genomic DNA. Translation: CAB15511.1.
PIRiF69611.
RefSeqiNP_391386.1. NC_000964.3.
WP_003244436.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NC3X-ray2.66A/B1-405[»]
3NC5X-ray2.90A/B1-405[»]
3NC6X-ray3.10A/B1-405[»]
3NC7X-ray3.30A/B1-405[»]
ProteinModelPortaliO34926.
SMRiO34926. Positions 4-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018976.

Proteomic databases

PaxDbiO34926.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15511; CAB15511; BSU35060.
GeneIDi936624.
KEGGibsu:BSU35060.
PATRICi18979018. VBIBacSub10457_3671.

Phylogenomic databases

eggNOGiENOG4106A3M. Bacteria.
COG2124. LUCA.
HOGENOMiHOG000243678.
InParanoidiO34926.
KOiK17474.
OMAiCTSEYEG.
PhylomeDBiO34926.

Enzyme and pathway databases

BioCyciBSUB:BSU35060-MONOMER.
MetaCyc:BSU35060-MONOMER.
BRENDAi1.14.15.13. 658.

Miscellaneous databases

EvolutionaryTraceiO34926.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030904. CypX.
IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
TIGRFAMsiTIGR04538. P450_cycloAA_1. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYPX_BACSU
AccessioniPrimary (citable) accession number: O34926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.