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Protein

Putative HMP/thiamine-binding protein YkoF

Gene

ykoF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex YkoCDEF that could transport hydroxymethylpyrimidine (HMP) and/or thiamine. Could also transport other HMP-containing products. Binds thiamine via its HMP moiety.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17Thiamine; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei49Thiamine1 Publication1

GO - Molecular functioni

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciBSUB:BSU13240-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative HMP/thiamine-binding protein YkoF
Gene namesi
Name:ykoF
Ordered Locus Names:BSU13240
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002888841 – 200Putative HMP/thiamine-binding protein YkoFAdd BLAST200

Proteomic databases

PaxDbiO34911
PRIDEiO34911

Interactioni

Subunit structurei

Homodimer in vitro. In vivo, may be a part of an ABC transporter complex which is composed of two ATP-binding proteins (YkoD), two transmembrane proteins (YkoC and YkoE) and a solute-binding protein (YkoF) (Probable).2 Publications

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007336

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Helixi24 – 34Combined sources11
Beta strandi40 – 44Combined sources5
Beta strandi49 – 53Combined sources5
Helixi55 – 70Combined sources16
Beta strandi76 – 84Combined sources9
Turni103 – 110Combined sources8
Beta strandi117 – 123Combined sources7
Helixi129 – 142Combined sources14
Beta strandi146 – 150Combined sources5
Beta strandi153 – 156Combined sources4
Helixi160 – 177Combined sources18
Beta strandi179 – 190Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S7HX-ray2.20A/B/C/D1-200[»]
1S99X-ray1.65A/B1-200[»]
1SBRX-ray2.30A/B1-200[»]
ProteinModelPortaliO34911
SMRiO34911
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34911

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108X43 Bacteria
ENOG4111NMW LUCA
HOGENOMiHOG000036207
OMAiCQFALYP

Family and domain databases

Gene3Di3.30.70.930, 2 hits
InterProiView protein in InterPro
IPR015835 HMP/thiamine-bd
IPR029756 MTH1187/YkoF-like
IPR011522 Thiamin/HMP-bd_put_YkoF
PfamiView protein in Pfam
PF07615 Ykof, 2 hits
PIRSFiPIRSF021331 YkoF, 1 hit
SUPFAMiSSF89957 SSF89957, 1 hit

Sequencei

Sequence statusi: Complete.

O34911-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHICGTSRI AGFRFSLYPM TDDFISVIKS ALKKTDTSKV WTKTDHISTV
60 70 80 90 100
LRGSIDHVFD AAKAIYLHAA NSEQHIVMNG TFSIGCPGDT QGDTYLSKGD
110 120 130 140 150
KRVNEDAVRG LKAEAPCQFA LYPMNEPDYM GLIMEAVDIA KAQGTFVQGV
160 170 180 190 200
HYASELDGDA HDVFSTLEAV FRMAEQQTNH ITMTVNLSAN SPSRKNRKQG
Length:200
Mass (Da):22,024
Last modified:January 1, 1998 - v1
Checksum:iA75C6662174F9465
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA Translation: CAA05603.1
AL009126 Genomic DNA Translation: CAB13181.1
PIRiB69859
RefSeqiNP_389207.1, NC_000964.3
WP_003244962.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13181; CAB13181; BSU13240
GeneIDi936465
KEGGibsu:BSU13240
PATRICifig|224308.179.peg.1438

Similar proteinsi

Entry informationi

Entry nameiYKOF_BACSU
AccessioniPrimary (citable) accession number: O34911
Secondary accession number(s): Q796L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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