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O34885

- YDIS_BACSU

UniProt

O34885 - YDIS_BACSU

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Protein

Type-2 restriction enzyme BsuMI component YdiS

Gene

ydiS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. ATP binding Source: InterPro
  3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BioCyciBSUB:BSU06100-MONOMER.

Protein family/group databases

REBASEi619. BsuMI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuMI component YdiS (EC:3.1.21.4)
Short name:
R.BsuM
Short name:
R.BsuMI
Alternative name(s):
Endonuclease BsuMI component YdiS
Type II restriction enzyme BsuMI component YdiS
Gene namesi
Name:ydiS
Ordered Locus Names:BSU06100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU06100.

Pathology & Biotechi

Disruption phenotypei

Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Type-2 restriction enzyme BsuMI component YdiSPRO_0000379882Add
BLAST

Proteomic databases

PaxDbiO34885.

Expressioni

Developmental stagei

Not expressed during sporulation.1 Publication

Inductioni

Constitutively expressed during exponential growth. Encoded in an operon with ydiR and ydjA.1 Publication

Interactioni

Subunit structurei

BsuMI restriction activity requires YdiR, YdiS and YdjA.

Protein-protein interaction databases

STRINGi224308.BSU06100.

Structurei

3D structure databases

ProteinModelPortaliO34885.
SMRiO34885. Positions 30-55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1401.
HOGENOMiHOG000080448.
InParanoidiO34885.
OMAiDKLLGPF.
OrthoDBiEOG69GZQ6.
PhylomeDBiO34885.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011704. ATPase_dyneun-rel_AAA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07728. AAA_5. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

O34885-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEISKQTSDL LLSLEKKKGT LPKFSVLRSI PRNRIIYGAP GTGKSNYLER
60 70 80 90 100
EVGKIFGDNP YVFTRVTFFP GYTYGQFIGA YKPVPIYKKL SGEEEIFSSN
110 120 130 140 150
FRDKMENFEP MIDYQFVPGP FIDVLIKALK NRYTNFILII EEINRANAAS
160 170 180 190 200
VFGDIFQLLD RNKNGESDYP VTFGPDIMNY LARNGIKDEM IKLPSNFFIW
210 220 230 240 250
ATMNNADQGV LPLDTAFKRR WSFEYLELEK YRKAVDSWKL SLRYKGHNKV
260 270 280 290 300
IMWNDFRDII NKRLKGKVPE DKLLGPFFLK ESELWNQNVF KNKLLYYLKE
310 320 330 340
DVFKHNPTID FLNASTFSEL IEKYDGSDNI FTFDIDDSSF VSD
Length:343
Mass (Da):40,033
Last modified:January 1, 1998 - v1
Checksum:i85A9A3D3AA66F2D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007637 Genomic DNA. Translation: BAA22754.1.
AL009126 Genomic DNA. Translation: CAB12429.1.
PIRiD69788.
RefSeqiNP_388491.1. NC_000964.3.
WP_003234052.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB12429; CAB12429; BSU06100.
GeneIDi938023.
KEGGibsu:BSU06100.
PATRICi18972856. VBIBacSub10457_0642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007637 Genomic DNA. Translation: BAA22754.1 .
AL009126 Genomic DNA. Translation: CAB12429.1 .
PIRi D69788.
RefSeqi NP_388491.1. NC_000964.3.
WP_003234052.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali O34885.
SMRi O34885. Positions 30-55.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU06100.

Protein family/group databases

REBASEi 619. BsuMI.

Proteomic databases

PaxDbi O34885.

Protocols and materials databases

DNASUi 938023.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB12429 ; CAB12429 ; BSU06100 .
GeneIDi 938023.
KEGGi bsu:BSU06100.
PATRICi 18972856. VBIBacSub10457_0642.

Organism-specific databases

GenoListi BSU06100.

Phylogenomic databases

eggNOGi COG1401.
HOGENOMi HOG000080448.
InParanoidi O34885.
OMAi DKLLGPF.
OrthoDBi EOG69GZQ6.
PhylomeDBi O34885.

Enzyme and pathway databases

BioCyci BSUB:BSU06100-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR011704. ATPase_dyneun-rel_AAA.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF07728. AAA_5. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
    Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
    DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
    Bron S., Janniere L., Ehrlich S.D.
    Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA TARGET SEQUENCE.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  4. "Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
    Ohshima H., Matsuoka S., Asai K., Sadaie Y.
    J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Entry informationi

Entry nameiYDIS_BACSU
AccessioniPrimary (citable) accession number: O34885
Secondary accession number(s): Q797C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

External Data

Dasty 3