Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O34885 (YDIS_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsuMI component YdiS

Short name=R.BsuM
Short name=R.BsuMI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsuMI component YdiS
Type II restriction enzyme BsuMI component YdiS
Gene names
Name:ydiS
Ordered Locus Names:BSU06100
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

BsuMI restriction activity requires YdiR, YdiS and YdjA.

Developmental stage

Not expressed during sporulation. Ref.4

Induction

Constitutively expressed during exponential growth. Encoded in an operon with ydiR and ydjA. Ref.4

Disruption phenotype

Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences. Ref.4

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343Type-2 restriction enzyme BsuMI component YdiS
PRO_0000379882

Sequences

Sequence LengthMass (Da)Tools
O34885 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 85A9A3D3AA66F2D9

FASTA34340,033
        10         20         30         40         50         60 
MEISKQTSDL LLSLEKKKGT LPKFSVLRSI PRNRIIYGAP GTGKSNYLER EVGKIFGDNP 

        70         80         90        100        110        120 
YVFTRVTFFP GYTYGQFIGA YKPVPIYKKL SGEEEIFSSN FRDKMENFEP MIDYQFVPGP 

       130        140        150        160        170        180 
FIDVLIKALK NRYTNFILII EEINRANAAS VFGDIFQLLD RNKNGESDYP VTFGPDIMNY 

       190        200        210        220        230        240 
LARNGIKDEM IKLPSNFFIW ATMNNADQGV LPLDTAFKRR WSFEYLELEK YRKAVDSWKL 

       250        260        270        280        290        300 
SLRYKGHNKV IMWNDFRDII NKRLKGKVPE DKLLGPFFLK ESELWNQNVF KNKLLYYLKE 

       310        320        330        340 
DVFKHNPTID FLNASTFSEL IEKYDGSDNI FTFDIDDSSF VSD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
Bron S., Janniere L., Ehrlich S.D.
Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA TARGET SEQUENCE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
Ohshima H., Matsuoka S., Asai K., Sadaie Y.
J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007637 Genomic DNA. Translation: BAA22754.1.
AL009126 Genomic DNA. Translation: CAB12429.1.
PIRD69788.
RefSeqNP_388491.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34885.
SMRO34885. Positions 30-55.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU06100.

Protein family/group databases

REBASE619. BsuMI.

Proteomic databases

PaxDbO34885.

Protocols and materials databases

DNASU938023.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12429; CAB12429; BSU06100.
GeneID938023.
KEGGbsu:BSU06100.
PATRIC18972856. VBIBacSub10457_0642.

Organism-specific databases

GenoListBSU06100.

Phylogenomic databases

eggNOGCOG1401.
HOGENOMHOG000080448.
OMADKLLGPF.
OrthoDBEOG69GZQ6.
PhylomeDBO34885.

Enzyme and pathway databases

BioCycBSUB:BSU06100-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR011704. ATPase_dyneun-rel_AAA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF07728. AAA_5. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
ProtoNetSearch...

Entry information

Entry nameYDIS_BACSU
AccessionPrimary (citable) accession number: O34885
Secondary accession number(s): Q797C9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList