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O34885

- YDIS_BACSU

UniProt

O34885 - YDIS_BACSU

Protein

Type-2 restriction enzyme BsuMI component YdiS

Gene

ydiS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. ATP binding Source: InterPro
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Enzyme and pathway databases

    BioCyciBSUB:BSU06100-MONOMER.

    Protein family/group databases

    REBASEi619. BsuMI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BsuMI component YdiS (EC:3.1.21.4)
    Short name:
    R.BsuM
    Short name:
    R.BsuMI
    Alternative name(s):
    Endonuclease BsuMI component YdiS
    Type II restriction enzyme BsuMI component YdiS
    Gene namesi
    Name:ydiS
    Ordered Locus Names:BSU06100
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU06100.

    Pathology & Biotechi

    Disruption phenotypei

    Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Type-2 restriction enzyme BsuMI component YdiSPRO_0000379882Add
    BLAST

    Proteomic databases

    PaxDbiO34885.

    Expressioni

    Developmental stagei

    Not expressed during sporulation.1 Publication

    Inductioni

    Constitutively expressed during exponential growth. Encoded in an operon with ydiR and ydjA.1 Publication

    Interactioni

    Subunit structurei

    BsuMI restriction activity requires YdiR, YdiS and YdjA.

    Protein-protein interaction databases

    STRINGi224308.BSU06100.

    Structurei

    3D structure databases

    ProteinModelPortaliO34885.
    SMRiO34885. Positions 30-55.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG1401.
    HOGENOMiHOG000080448.
    OMAiDKLLGPF.
    OrthoDBiEOG69GZQ6.
    PhylomeDBiO34885.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR011704. ATPase_dyneun-rel_AAA.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF07728. AAA_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    O34885-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEISKQTSDL LLSLEKKKGT LPKFSVLRSI PRNRIIYGAP GTGKSNYLER    50
    EVGKIFGDNP YVFTRVTFFP GYTYGQFIGA YKPVPIYKKL SGEEEIFSSN 100
    FRDKMENFEP MIDYQFVPGP FIDVLIKALK NRYTNFILII EEINRANAAS 150
    VFGDIFQLLD RNKNGESDYP VTFGPDIMNY LARNGIKDEM IKLPSNFFIW 200
    ATMNNADQGV LPLDTAFKRR WSFEYLELEK YRKAVDSWKL SLRYKGHNKV 250
    IMWNDFRDII NKRLKGKVPE DKLLGPFFLK ESELWNQNVF KNKLLYYLKE 300
    DVFKHNPTID FLNASTFSEL IEKYDGSDNI FTFDIDDSSF VSD 343
    Length:343
    Mass (Da):40,033
    Last modified:January 1, 1998 - v1
    Checksum:i85A9A3D3AA66F2D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007637 Genomic DNA. Translation: BAA22754.1.
    AL009126 Genomic DNA. Translation: CAB12429.1.
    PIRiD69788.
    RefSeqiNP_388491.1. NC_000964.3.
    WP_003234052.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12429; CAB12429; BSU06100.
    GeneIDi938023.
    KEGGibsu:BSU06100.
    PATRICi18972856. VBIBacSub10457_0642.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007637 Genomic DNA. Translation: BAA22754.1 .
    AL009126 Genomic DNA. Translation: CAB12429.1 .
    PIRi D69788.
    RefSeqi NP_388491.1. NC_000964.3.
    WP_003234052.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali O34885.
    SMRi O34885. Positions 30-55.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU06100.

    Protein family/group databases

    REBASEi 619. BsuMI.

    Proteomic databases

    PaxDbi O34885.

    Protocols and materials databases

    DNASUi 938023.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12429 ; CAB12429 ; BSU06100 .
    GeneIDi 938023.
    KEGGi bsu:BSU06100.
    PATRICi 18972856. VBIBacSub10457_0642.

    Organism-specific databases

    GenoListi BSU06100.

    Phylogenomic databases

    eggNOGi COG1401.
    HOGENOMi HOG000080448.
    OMAi DKLLGPF.
    OrthoDBi EOG69GZQ6.
    PhylomeDBi O34885.

    Enzyme and pathway databases

    BioCyci BSUB:BSU06100-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR011704. ATPase_dyneun-rel_AAA.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF07728. AAA_5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
      Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
      DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
      Bron S., Janniere L., Ehrlich S.D.
      Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA TARGET SEQUENCE.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    4. "Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
      Ohshima H., Matsuoka S., Asai K., Sadaie Y.
      J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

    Entry informationi

    Entry nameiYDIS_BACSU
    AccessioniPrimary (citable) accession number: O34885
    Secondary accession number(s): Q797C9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

    External Data

    Dasty 3