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O34874 (ISCS2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative cysteine desulfurase IscS 2
EC=2.8.1.7
Gene names
Name:iscS2
Synonyms:nifS2
Ordered Locus Names:BSU29590
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the removal of elemental sulfur from cysteine to produce alanine By similarity.

Catalytic activity

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.

Cofactor

Pyridoxal phosphate By similarity.

Sequence similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.

Ontologies

Keywords
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functioncysteine desulfurase activity

Inferred from electronic annotation. Source: EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Putative cysteine desulfurase IscS 2
PRO_0000386537

Sites

Active site3241 By similarity

Amino acid modifications

Modified residue2021N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
O34874 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B7C7231E5A79F8FE

FASTA38141,488
        10         20         30         40         50         60 
MIYLDNSSTT KPYDEVLNVY EQTSSRYFGN PSSLHRYGAE TEQLLQAAKN QIKRSLGLKK 

        70         80         90        100        110        120 
YDIVFTSGAT EANNAALKGA ALSKIKTGKH IIATSIEHPS VTESLEQLKE LFGFDITYLS 

       130        140        150        160        170        180 
VNEDGFVSIE DLKAAIRPDT VLVSMMHVNN EVGSVQPIEA AGEVLKEHSN ILFHVDYVQG 

       190        200        210        220        230        240 
IYKVPLAIEK AGIDLCSISG HKFHGLKGTG ALIVKEGTRL IPLITGGSQQ KGIRAGTEHT 

       250        260        270        280        290        300 
AGAVSLAKAI NLASADFDTR LDTMTAVKEL FMNRLSEIEG VVINTPQMNS APHIINFSVP 

       310        320        330        340        350        360 
GIKAEVLLHM LEEQDIFVST TSACSAKEHK PSKVLLEMGK GEQIAGSSIR ISLNYSQTSD 

       370        380 
VAEPFMNALR PGIKKLREMM R

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF008220 Genomic DNA. Translation: AAC00307.1.
AL009126 Genomic DNA. Translation: CAB14937.1.
PIRF69666.
RefSeqNP_390837.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34874.
SMRO34874. Positions 1-380.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU29590.

Proteomic databases

PaxDbO34874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14937; CAB14937; BSU29590.
GeneID937701.
KEGGbsu:BSU29590.
PATRIC18977822. VBIBacSub10457_3099.

Organism-specific databases

GenoListBSU29590. [Micado]

Phylogenomic databases

eggNOGCOG1104.
HOGENOMHOG000017510.
KOK04487.
OMANSAPHII.
ProtClustDBCLSK887724.

Enzyme and pathway databases

BioCycBSUB:BSU29590-MONOMER.
MetaCyc:BSU29590-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR000192. Aminotrans_V/Cys_dSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFPIRSF005572. NifS. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISCS2_BACSU
AccessionPrimary (citable) accession number: O34874
Secondary accession number(s): Q795T7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: January 1, 1998
Last modified: May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents