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Protein

L-cystine-binding protein TcyK

Gene

tcyK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex TcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU29370-MONOMER.

Protein family/group databases

TCDBi3.A.1.3.13. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cystine-binding protein TcyK
Gene namesi
Name:tcyK
Synonyms:ytmK
Ordered Locus Names:BSU29370
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20PROSITE-ProRule annotationAdd BLAST20
ChainiPRO_000003178021 – 270L-cystine-binding protein TcyKAdd BLAST250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi21N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi21S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiO34852.

Expressioni

Inductioni

More strongly expressed in the presence of methionine than in the presence of sulfate.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL and TcyM) and two solute-binding proteins (TcyJ and TcyK).Curated

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016031.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 42Combined sources7
Beta strandi44 – 46Combined sources3
Turni47 – 49Combined sources3
Beta strandi50 – 52Combined sources3
Beta strandi58 – 60Combined sources3
Helixi61 – 72Combined sources12
Beta strandi76 – 82Combined sources7
Helixi85 – 87Combined sources3
Helixi88 – 93Combined sources6
Beta strandi96 – 104Combined sources9
Helixi108 – 113Combined sources6
Beta strandi122 – 131Combined sources10
Helixi140 – 142Combined sources3
Beta strandi147 – 150Combined sources4
Beta strandi152 – 154Combined sources3
Helixi155 – 166Combined sources12
Beta strandi171 – 175Combined sources5
Helixi182 – 189Combined sources8
Beta strandi193 – 197Combined sources5
Helixi199 – 208Combined sources10
Beta strandi213 – 229Combined sources17
Helixi234 – 249Combined sources16
Helixi252 – 260Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O1MX-ray2.00A/B22-270[»]
ProteinModelPortaliO34852.
SMRiO34852.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34852.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0834. LUCA.
HOGENOMiHOG000031895.
InParanoidiO34852.
KOiK16957.
OMAiMFNKNEQ.
PhylomeDBiO34852.

Family and domain databases

InterProiIPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O34852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKTAFMAI LFSLITVLSA CGAGSQTTGA GQKKVQTITV GTGTQFPNIC
60 70 80 90 100
FIDEKGDLTG YDVELIKELD KRLPHYKFTF KTMEFSNLLV SLGQHKVDIV
110 120 130 140 150
AHQMEKSKER EKKFLFNKVA YNHFPLKITV LQNNDTIRGI EDLKGKRVIT
160 170 180 190 200
SATSNGALVL KKWNEDNGRP FEIAYEGQGA NETANQLKSG RADATISTPF
210 220 230 240 250
AVDFQNKTST IKEKTVGNVL SNAKVYFMFN KNEQTLSDDI DKALQEIIDD
260 270
GTLKRLSLKW LGDDYSKEQY
Length:270
Mass (Da):30,241
Last modified:January 1, 1998 - v1
Checksum:i6F16029F7B9C6638
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00326.1.
AL009126 Genomic DNA. Translation: CAB14897.1.
PIRiE69996.
RefSeqiNP_390815.1. NC_000964.3.
WP_004399116.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14897; CAB14897; BSU29370.
GeneIDi937359.
KEGGibsu:BSU29370.
PATRICi18977774. VBIBacSub10457_3075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00326.1.
AL009126 Genomic DNA. Translation: CAB14897.1.
PIRiE69996.
RefSeqiNP_390815.1. NC_000964.3.
WP_004399116.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O1MX-ray2.00A/B22-270[»]
ProteinModelPortaliO34852.
SMRiO34852.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016031.

Protein family/group databases

TCDBi3.A.1.3.13. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiO34852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14897; CAB14897; BSU29370.
GeneIDi937359.
KEGGibsu:BSU29370.
PATRICi18977774. VBIBacSub10457_3075.

Phylogenomic databases

eggNOGiCOG0834. LUCA.
HOGENOMiHOG000031895.
InParanoidiO34852.
KOiK16957.
OMAiMFNKNEQ.
PhylomeDBiO34852.

Enzyme and pathway databases

BioCyciBSUB:BSU29370-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO34852.

Family and domain databases

InterProiIPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCYK_BACSU
AccessioniPrimary (citable) accession number: O34852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.