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O34825 (PKSC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksC
Short name=AT
EC=2.3.1.39
Gene names
Name:pksC
Ordered Locus Names:BSU17100
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the transfer of the malonyl-CoA group to the acyl-carrier-protein AcpK (Mal-AcpK). Ref.2 Ref.4

Catalytic activity

Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein]. Ref.2

Pathway

Antibiotic biosynthesis; bacillaene biosynthesis.

Subcellular location

Cytoplasm Ref.3.

Sequence similarities

Belongs to the FabD family.

Ontologies

Keywords
   Biological processAntibiotic biosynthesis
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantibiotic biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function[acyl-carrier-protein] S-malonyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksC
PRO_0000388004

Sites

Active site871 By similarity
Active site1931 By similarity

Sequences

Sequence LengthMass (Da)Tools
O34825 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: AFAE397EAB2FD3C6

FASTA28832,464
        10         20         30         40         50         60 
MITYVFPGQG SQKQGMGSGL FDEFKELTDQ ADEILGYSIK RLCLENPYSN LNKTQFTQPA 

        70         80         90        100        110        120 
LYVVNALSYL KKIRDEEVKP DFVAGHSLGE YNALFAAEAF DFETGLQLVR KRGELMSLIS 

       130        140        150        160        170        180 
NGGMAAVMGL NEEQVAKALK EYHLHDVDIA NVNAPYQIVI SGKKDEIEKA ASLFETMTEV 

       190        200        210        220        230        240 
TMVLPLNVSG AFHSRYMNKA KEEFEEFLHA FYFSPPSIPV ISNVYAKPYT YEFMKQTLAD 

       250        260        270        280 
QINHSVKWTD SISYLMKKGH MEFEEVGPGN VLTGLIHRIK KDAEAMPR

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[2]"Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis."
Calderone C.T., Kowtoniuk W.E., Kelleher N.L., Walsh C.T., Dorrestein P.C.
Proc. Natl. Acad. Sci. U.S.A. 103:8977-8982(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
[3]"A singular enzymatic megacomplex from Bacillus subtilis."
Straight P.D., Fischbach M.A., Walsh C.T., Rudner D.Z., Kolter R.
Proc. Natl. Acad. Sci. U.S.A. 104:305-310(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis."
Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R., Walsh C.T., Clardy J.
Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN BACILLAENE BIOSYNTHESIS.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL009126 Genomic DNA. Translation: CAB13582.1.
PIRA69678.
RefSeqNP_389591.1. NC_000964.3.

3D structure databases

HSSPHSSP built from PDB template 2G2Z based on UniProtKB P0AAI9.
ProteinModelPortalO34825.
SMRO34825. Positions 1-285.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU17100.

Proteomic databases

PaxDbO34825.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13582; CAB13582; BSU17100.
GeneID939504.
KEGGbsu:BSU17100.
PATRIC18975227. VBIBacSub10457_1805.

Organism-specific databases

GenoListBSU17100. [Micado]

Phylogenomic databases

eggNOGCOG0331.
HOGENOMHOG000036504.
KOK15327.
OMACLENPYS.
ProtClustDBCLSK887323.

Enzyme and pathway databases

BioCycBSUB:BSU17100-MONOMER.
UniPathwayUPA01003.

Family and domain databases

Gene3D3.40.366.10. 2 hits.
InterProIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR024925. Malonyl_CoA-ACP_transAc.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
PIRSFPIRSF000446. Mct. 1 hit.
SUPFAMSSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.
TIGRFAMsTIGR00128. fabD. 1 hit.
ProtoNetSearch...

Entry information

Entry namePKSC_BACSU
AccessionPrimary (citable) accession number: O34825
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: January 1, 1998
Last modified: May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents