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O34825

- PKSC_BACSU

UniProt

O34825 - PKSC_BACSU

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Protein

Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksC

Gene

pksC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the transfer of the malonyl-CoA group to the acyl-carrier-protein AcpK (Mal-AcpK).2 Publications

Catalytic activityi

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871By similarity
Active sitei193 – 1931By similarity

GO - Molecular functioni

  1. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU17100-MONOMER.
UniPathwayiUPA01003.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksC (EC:2.3.1.39)
Short name:
AT
Gene namesi
Name:pksC
Ordered Locus Names:BSU17100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU17100. [Micado]

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksCPRO_0000388004Add
BLAST

Proteomic databases

PaxDbiO34825.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU17100.

Structurei

3D structure databases

ProteinModelPortaliO34825.
SMRiO34825. Positions 1-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FabD family.Curated

Phylogenomic databases

eggNOGiCOG0331.
HOGENOMiHOG000036504.
InParanoidiO34825.
KOiK15327.
OMAiCLENPYS.
OrthoDBiEOG6W19KW.
PhylomeDBiO34825.

Family and domain databases

Gene3Di3.40.366.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR024925. Malonyl_CoA-ACP_transAc.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000446. Mct. 1 hit.
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsiTIGR00128. fabD. 1 hit.

Sequencei

Sequence statusi: Complete.

O34825-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITYVFPGQG SQKQGMGSGL FDEFKELTDQ ADEILGYSIK RLCLENPYSN
60 70 80 90 100
LNKTQFTQPA LYVVNALSYL KKIRDEEVKP DFVAGHSLGE YNALFAAEAF
110 120 130 140 150
DFETGLQLVR KRGELMSLIS NGGMAAVMGL NEEQVAKALK EYHLHDVDIA
160 170 180 190 200
NVNAPYQIVI SGKKDEIEKA ASLFETMTEV TMVLPLNVSG AFHSRYMNKA
210 220 230 240 250
KEEFEEFLHA FYFSPPSIPV ISNVYAKPYT YEFMKQTLAD QINHSVKWTD
260 270 280
SISYLMKKGH MEFEEVGPGN VLTGLIHRIK KDAEAMPR
Length:288
Mass (Da):32,464
Last modified:January 1, 1998 - v1
Checksum:iAFAE397EAB2FD3C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13582.1.
PIRiA69678.
RefSeqiNP_389591.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13582; CAB13582; BSU17100.
GeneIDi939504.
KEGGibsu:BSU17100.
PATRICi18975227. VBIBacSub10457_1805.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13582.1 .
PIRi A69678.
RefSeqi NP_389591.1. NC_000964.3.

3D structure databases

ProteinModelPortali O34825.
SMRi O34825. Positions 1-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU17100.

Proteomic databases

PaxDbi O34825.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13582 ; CAB13582 ; BSU17100 .
GeneIDi 939504.
KEGGi bsu:BSU17100.
PATRICi 18975227. VBIBacSub10457_1805.

Organism-specific databases

GenoListi BSU17100. [Micado ]

Phylogenomic databases

eggNOGi COG0331.
HOGENOMi HOG000036504.
InParanoidi O34825.
KOi K15327.
OMAi CLENPYS.
OrthoDBi EOG6W19KW.
PhylomeDBi O34825.

Enzyme and pathway databases

UniPathwayi UPA01003 .
BioCyci BSUB:BSU17100-MONOMER.

Family and domain databases

Gene3Di 3.40.366.10. 2 hits.
InterProi IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR024925. Malonyl_CoA-ACP_transAc.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000446. Mct. 1 hit.
SUPFAMi SSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsi TIGR00128. fabD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis."
    Calderone C.T., Kowtoniuk W.E., Kelleher N.L., Walsh C.T., Dorrestein P.C.
    Proc. Natl. Acad. Sci. U.S.A. 103:8977-8982(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  3. Cited for: SUBCELLULAR LOCATION.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  4. "The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis."
    Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R., Walsh C.T., Clardy J.
    Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BACILLAENE BIOSYNTHESIS.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Entry informationi

Entry nameiPKSC_BACSU
AccessioniPrimary (citable) accession number: O34825
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3