Reviewed, UniProtKB/Swiss-Prot O34819 (PLY1_BACSU)

Names and origin

Protein names

Recommended name:
    Pectin lyase
      Short name=PNL
    EC=4.2.2.10

Gene names

Name:	pelB
Ordered Locus Names:	BSU18650

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the depolymerization of pectins of methyl esterification degree from 13 to 75%, with an endo mode of action. Can not degrade polygalacturonate By similarity.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	pectin lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	By similarity
	Chain	25 – 345	321	Pectin lyase		PRO_0000367323
Sites
	Active site	234	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O34819-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E84A968D886F5A5D	FASTA	345	38,108
	10 20 30 40 50 60 MKRLCLWFTV FSLFLVLLPG KALGAVDFPN TSTNGLLGFA GNAKNEKGIS KTGTTGGKNG 70 80 90 100 110 120 QIVYIQSVND LKTHLGGSTP KILVLQNDLS ASAKTTVTIG SNKTLVGSYA KKTLKNIYLT 130 140 150 160 170 180 TSSAFGNVIF QNLTFEHSPQ INGNNDIQLY LDSGMNYWID HVTFSGHSYN ASGSDLDKLV 190 200 210 220 230 240 YIGKSADYIT ISNSKFANHK YGLILGYPDD SQHQYDGYPH MTIANNYFEN LYVRGPGLMR 250 260 270 280 290 300 YGYFHVKNNY SNNFNQAITI ATKAKIYSEY NYFGKGSEKG GILDDKGTGY FKDIGSYPSL 310 320 330 340 NKQTSPLTSW NPGSNYSYRV QTPEYTKEFV TKYAGSQSTT LVFGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the Bacillus subtilis chromosome region between the terC and odhAB loci cloned in a yeast artificial chromosome." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF027868 Genomic DNA. Translation: AAB84422.1. AL009126 Genomic DNA. Translation: CAB13757.1.
PIR	E69674.
RefSeq	NP_389746.1.
3D structure databases
HSSP	HSSP built from PDB template 1BN8 based on UniProtKB P39116.
ModBase	Search...
Genome annotation databases
GeneID	940111.
GenomeReviews	Gene locus BSU18650 in contig AL009126_GR.
KEGG	bsu:BSU18650.
NMPDR	fig|224308.1.peg.1868.
Organism-specific databases
SubtiList	BG12643. pelB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O34819.
OMA	GLMRYGY.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1864-MON.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLY1_BACSU

Accession

Primary (citable) accession number: O34819
Secondary accession number(s): Q796F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)