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O34787 (PKSE_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyketide biosynthesis protein PksE

Including the following 1 domains:

  1. Malonyl CoA-acyl carrier protein transacylase
    Short name=MCT
    EC=2.3.1.39
Gene names
Name:pksE
Ordered Locus Names:BSU17120
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length767 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductase activity. Ref.4

Catalytic activity

Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].

Pathway

Antibiotic biosynthesis; bacillaene biosynthesis.

Subcellular location

Cytoplasm Ref.3.

Sequence similarities

In the N-terminal section; belongs to the FabD family.

Ontologies

Keywords
   Biological processAntibiotic biosynthesis
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantibiotic biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function[acyl-carrier-protein] S-malonyltransferase activity

Inferred from electronic annotation. Source: EC

nitronate monooxygenase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 767767Polyketide biosynthesis protein PksE
PRO_0000388002

Regions

Region1 – 312312Acyl transferase

Sites

Active site871 By similarity
Active site1931 By similarity

Sequences

Sequence LengthMass (Da)Tools
O34787 [UniParc].

Last modified May 5, 2009. Version 3.
Checksum: C97C8825E3DE8881

FASTA76785,743
        10         20         30         40         50         60 
MITYVFPGQG SQQKGMGQGL FEQYQHLTDQ ADQILGYSIE KLCTEKSYLD VNHTEYTQPA 

        70         80         90        100        110        120 
LYVVNALSYL KRVEETGRKP DFAAGHSLGE YNALMAAGAF DFETGLRLVK KRGELMGRIT 

       130        140        150        160        170        180 
GGGMAAVIGL SKEQVTAVLE EHRLYDIDVA NENTPQQIVI SGPKKEIEKA RAVFENTKDV 

       190        200        210        220        230        240 
KLFHPLNVSG AFHSRYMNEA KQVFKQYIDS FQFAPLAIPV ISNVYAEPYH QDRLKDTLSE 

       250        260        270        280        290        300 
QMDNTVKWTD SIRFLMGRGE MEFAEIGPGT VLTGLIHRIK NEAEPLTYIP KKNPAISAHL 

       310        320        330        340        350        360 
KEQRNVQAGI TAESLGSAEF KQDYHLTYAY LAGGMYRGIA SKEMVVKLSR AGMMGFFGTG 

       370        380        390        400        410        420 
GLSLKEVEDA IHAIQGELGK GQAYGINLVH NMKHTESEEK MIDLLLRNQV SIVEASAFLS 

       430        440        450        460        470        480 
VTPVLVRYRA KGVKRNQNGD VICSNRLIAK ISRPEVAESF LSPAPENMLQ KLLGENKITM 

       490        500        510        520        530        540 
NEAELLRCIP MADDICVEAD SGGHTDGGVA YSLMPAMTSL RDEMMKKYQY RKKIRVGAAG 

       550        560        570        580        590        600 
GIGTPEAAMA AFMLGADFIL TGSINQCTVE AATSDKVKDL LQQMNVQDTA YAPAGDMFES 

       610        620        630        640        650        660 
GSKVQVLKKG VFFPARANKL YELYQRYGSI RELDAKMLAQ LEEKYFKRSI EDIYKDIALH 

       670        680        690        700        710        720 
YPAADIEKAE QNPKHKMALI FRWYFRYSSK LAISGSEHSK VDYQIHCGPA LGAFNQWVKG 

       730        740        750        760 
SQLENWRNRH VDEIGKKLMT ETAVLLHERM QSMYQPSHET DNIKIKV

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[2]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 99-129.
[3]"A singular enzymatic megacomplex from Bacillus subtilis."
Straight P.D., Fischbach M.A., Walsh C.T., Rudner D.Z., Kolter R.
Proc. Natl. Acad. Sci. U.S.A. 104:305-310(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis."
Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R., Walsh C.T., Clardy J.
Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN BACILLAENE BIOSYNTHESIS.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL009126 Genomic DNA. Translation: CAB13584.3.
RefSeqNP_389593.3. NC_000964.3.

3D structure databases

HSSPHSSP built from PDB template 2G2Z based on UniProtKB P0AAI9.
ProteinModelPortalO34787.
SMRO34787. Positions 1-283, 480-586.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU17120.

Proteomic databases

PaxDbO34787.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13584; CAB13584; BSU17120.
GeneID939997.
KEGGbsu:BSU17120.
PATRIC18975233. VBIBacSub10457_1808.

Organism-specific databases

GenoListBSU17120. [Micado]

Phylogenomic databases

eggNOGCOG0331.
HOGENOMHOG000275975.
KOK15329.
ProtClustDBCLSK928131.

Enzyme and pathway databases

BioCycBSUB:BSU17120-MONOMER.
UniPathwayUPA01003.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
3.40.366.10. 2 hits.
InterProIPR004136. 2Npropane_dOase.
IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013785. Aldolase_TIM.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR014179. PfaD_fam.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
PF03060. NMO. 1 hit.
[Graphical view]
SUPFAMSSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.
TIGRFAMsTIGR00128. fabD. 1 hit.
TIGR02814. pfaD_fam. 1 hit.
ProtoNetSearch...

Entry information

Entry namePKSE_BACSU
AccessionPrimary (citable) accession number: O34787
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: May 1, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents