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O34787

- PKSE_BACSU

UniProt

O34787 - PKSE_BACSU

Protein

Polyketide biosynthesis protein PksE

Gene

pksE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 3 (05 May 2009)
      Previous versions | rss
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    Functioni

    Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductase activity.1 Publication

    Catalytic activityi

    Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei87 – 871By similarity
    Active sitei193 – 1931By similarity

    GO - Molecular functioni

    1. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC
    2. nitronate monooxygenase activity Source: InterPro

    GO - Biological processi

    1. antibiotic biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciBSUB:BSU17120-MONOMER.
    UniPathwayiUPA01003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyketide biosynthesis protein PksE
    Including the following 1 domains:
    Malonyl CoA-acyl carrier protein transacylase (EC:2.3.1.39)
    Short name:
    MCT
    Gene namesi
    Name:pksE
    Ordered Locus Names:BSU17120
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU17120. [Micado]

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 767767Polyketide biosynthesis protein PksEPRO_0000388002Add
    BLAST

    Proteomic databases

    PaxDbiO34787.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU17120.

    Structurei

    3D structure databases

    ProteinModelPortaliO34787.
    SMRiO34787. Positions 1-283, 480-586.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 312312Acyl transferaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the FabD family.Curated

    Phylogenomic databases

    eggNOGiCOG0331.
    HOGENOMiHOG000275975.
    KOiK15329.
    OrthoDBiEOG67X1P6.
    PhylomeDBiO34787.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    3.40.366.10. 2 hits.
    InterProiIPR004136. 2Npropane_dOase.
    IPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013785. Aldolase_TIM.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR014179. PfaD_fam.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF03060. NMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsiTIGR00128. fabD. 1 hit.
    TIGR02814. pfaD_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O34787-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITYVFPGQG SQQKGMGQGL FEQYQHLTDQ ADQILGYSIE KLCTEKSYLD    50
    VNHTEYTQPA LYVVNALSYL KRVEETGRKP DFAAGHSLGE YNALMAAGAF 100
    DFETGLRLVK KRGELMGRIT GGGMAAVIGL SKEQVTAVLE EHRLYDIDVA 150
    NENTPQQIVI SGPKKEIEKA RAVFENTKDV KLFHPLNVSG AFHSRYMNEA 200
    KQVFKQYIDS FQFAPLAIPV ISNVYAEPYH QDRLKDTLSE QMDNTVKWTD 250
    SIRFLMGRGE MEFAEIGPGT VLTGLIHRIK NEAEPLTYIP KKNPAISAHL 300
    KEQRNVQAGI TAESLGSAEF KQDYHLTYAY LAGGMYRGIA SKEMVVKLSR 350
    AGMMGFFGTG GLSLKEVEDA IHAIQGELGK GQAYGINLVH NMKHTESEEK 400
    MIDLLLRNQV SIVEASAFLS VTPVLVRYRA KGVKRNQNGD VICSNRLIAK 450
    ISRPEVAESF LSPAPENMLQ KLLGENKITM NEAELLRCIP MADDICVEAD 500
    SGGHTDGGVA YSLMPAMTSL RDEMMKKYQY RKKIRVGAAG GIGTPEAAMA 550
    AFMLGADFIL TGSINQCTVE AATSDKVKDL LQQMNVQDTA YAPAGDMFES 600
    GSKVQVLKKG VFFPARANKL YELYQRYGSI RELDAKMLAQ LEEKYFKRSI 650
    EDIYKDIALH YPAADIEKAE QNPKHKMALI FRWYFRYSSK LAISGSEHSK 700
    VDYQIHCGPA LGAFNQWVKG SQLENWRNRH VDEIGKKLMT ETAVLLHERM 750
    QSMYQPSHET DNIKIKV 767
    Length:767
    Mass (Da):85,743
    Last modified:May 5, 2009 - v3
    Checksum:iC97C8825E3DE8881
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB13584.3.
    RefSeqiNP_389593.3. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13584; CAB13584; BSU17120.
    GeneIDi939997.
    KEGGibsu:BSU17120.
    PATRICi18975233. VBIBacSub10457_1808.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB13584.3 .
    RefSeqi NP_389593.3. NC_000964.3.

    3D structure databases

    ProteinModelPortali O34787.
    SMRi O34787. Positions 1-283, 480-586.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU17120.

    Proteomic databases

    PaxDbi O34787.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13584 ; CAB13584 ; BSU17120 .
    GeneIDi 939997.
    KEGGi bsu:BSU17120.
    PATRICi 18975233. VBIBacSub10457_1808.

    Organism-specific databases

    GenoListi BSU17120. [Micado ]

    Phylogenomic databases

    eggNOGi COG0331.
    HOGENOMi HOG000275975.
    KOi K15329.
    OrthoDBi EOG67X1P6.
    PhylomeDBi O34787.

    Enzyme and pathway databases

    UniPathwayi UPA01003 .
    BioCyci BSUB:BSU17120-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    3.40.366.10. 2 hits.
    InterProi IPR004136. 2Npropane_dOase.
    IPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013785. Aldolase_TIM.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR014179. PfaD_fam.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF03060. NMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsi TIGR00128. fabD. 1 hit.
    TIGR02814. pfaD_fam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    2. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 99-129.
    3. Cited for: SUBCELLULAR LOCATION.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    4. "The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis."
      Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R., Walsh C.T., Clardy J.
      Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN BACILLAENE BIOSYNTHESIS.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

    Entry informationi

    Entry nameiPKSE_BACSU
    AccessioniPrimary (citable) accession number: O34787
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 92 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3