SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O34787

- PKSE_BACSU

UniProt

O34787 - PKSE_BACSU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Polyketide biosynthesis protein PksE
Gene
pksE, BSU17120
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductase activity.1 Publication

Catalytic activityi

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871 By similarity
Active sitei193 – 1931 By similarity

GO - Molecular functioni

  1. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC
  2. nitronate monooxygenase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU17120-MONOMER.
UniPathwayiUPA01003.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyketide biosynthesis protein PksE
Including the following 1 domains:
Malonyl CoA-acyl carrier protein transacylase (EC:2.3.1.39)
Short name:
MCT
Gene namesi
Name:pksE
Ordered Locus Names:BSU17120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU17120. [Micado]

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Polyketide biosynthesis protein PksE
PRO_0000388002Add
BLAST

Proteomic databases

PaxDbiO34787.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU17120.

Structurei

3D structure databases

ProteinModelPortaliO34787.
SMRiO34787. Positions 1-283, 480-586.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 312312Acyl transferase
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the FabD family.

Phylogenomic databases

eggNOGiCOG0331.
HOGENOMiHOG000275975.
KOiK15329.
OrthoDBiEOG67X1P6.
PhylomeDBiO34787.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
3.40.366.10. 2 hits.
InterProiIPR004136. 2Npropane_dOase.
IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013785. Aldolase_TIM.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR014179. PfaD_fam.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF03060. NMO. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsiTIGR00128. fabD. 1 hit.
TIGR02814. pfaD_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

O34787-1 [UniParc]FASTAAdd to Basket

« Hide

MITYVFPGQG SQQKGMGQGL FEQYQHLTDQ ADQILGYSIE KLCTEKSYLD    50
VNHTEYTQPA LYVVNALSYL KRVEETGRKP DFAAGHSLGE YNALMAAGAF 100
DFETGLRLVK KRGELMGRIT GGGMAAVIGL SKEQVTAVLE EHRLYDIDVA 150
NENTPQQIVI SGPKKEIEKA RAVFENTKDV KLFHPLNVSG AFHSRYMNEA 200
KQVFKQYIDS FQFAPLAIPV ISNVYAEPYH QDRLKDTLSE QMDNTVKWTD 250
SIRFLMGRGE MEFAEIGPGT VLTGLIHRIK NEAEPLTYIP KKNPAISAHL 300
KEQRNVQAGI TAESLGSAEF KQDYHLTYAY LAGGMYRGIA SKEMVVKLSR 350
AGMMGFFGTG GLSLKEVEDA IHAIQGELGK GQAYGINLVH NMKHTESEEK 400
MIDLLLRNQV SIVEASAFLS VTPVLVRYRA KGVKRNQNGD VICSNRLIAK 450
ISRPEVAESF LSPAPENMLQ KLLGENKITM NEAELLRCIP MADDICVEAD 500
SGGHTDGGVA YSLMPAMTSL RDEMMKKYQY RKKIRVGAAG GIGTPEAAMA 550
AFMLGADFIL TGSINQCTVE AATSDKVKDL LQQMNVQDTA YAPAGDMFES 600
GSKVQVLKKG VFFPARANKL YELYQRYGSI RELDAKMLAQ LEEKYFKRSI 650
EDIYKDIALH YPAADIEKAE QNPKHKMALI FRWYFRYSSK LAISGSEHSK 700
VDYQIHCGPA LGAFNQWVKG SQLENWRNRH VDEIGKKLMT ETAVLLHERM 750
QSMYQPSHET DNIKIKV 767
Length:767
Mass (Da):85,743
Last modified:May 5, 2009 - v3
Checksum:iC97C8825E3DE8881
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL009126 Genomic DNA. Translation: CAB13584.3.
RefSeqiNP_389593.3. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13584; CAB13584; BSU17120.
GeneIDi939997.
KEGGibsu:BSU17120.
PATRICi18975233. VBIBacSub10457_1808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL009126 Genomic DNA. Translation: CAB13584.3 .
RefSeqi NP_389593.3. NC_000964.3.

3D structure databases

ProteinModelPortali O34787.
SMRi O34787. Positions 1-283, 480-586.
ModBasei Search...

Protein-protein interaction databases

STRINGi 224308.BSU17120.

Proteomic databases

PaxDbi O34787.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13584 ; CAB13584 ; BSU17120 .
GeneIDi 939997.
KEGGi bsu:BSU17120.
PATRICi 18975233. VBIBacSub10457_1808.

Organism-specific databases

GenoListi BSU17120. [Micado ]

Phylogenomic databases

eggNOGi COG0331.
HOGENOMi HOG000275975.
KOi K15329.
OrthoDBi EOG67X1P6.
PhylomeDBi O34787.

Enzyme and pathway databases

UniPathwayi UPA01003 .
BioCyci BSUB:BSU17120-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
3.40.366.10. 2 hits.
InterProi IPR004136. 2Npropane_dOase.
IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013785. Aldolase_TIM.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR014179. PfaD_fam.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF03060. NMO. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsi TIGR00128. fabD. 1 hit.
TIGR02814. pfaD_fam. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 99-129.
  3. Cited for: SUBCELLULAR LOCATION.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  4. "The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis."
    Butcher R.A., Schroeder F.C., Fischbach M.A., Straight P.D., Kolter R., Walsh C.T., Clardy J.
    Proc. Natl. Acad. Sci. U.S.A. 104:1506-1509(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BACILLAENE BIOSYNTHESIS.
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Entry informationi

Entry nameiPKSE_BACSU
AccessioniPrimary (citable) accession number: O34787
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: September 3, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi