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Protein

Organic hydroperoxide resistance transcriptional regulator

Gene

ohrR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Organic peroxide sensor. Represses the expression of the peroxide-inducible gene ohrA by cooperative binding to two inverted repeat elements.1 Publication

Enzyme regulationi

Inactivated by oxidation of Cys-15 to a sulfenic acid.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi57 – 80H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU13150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Organic hydroperoxide resistance transcriptional regulator
Gene namesi
Name:ohrR
Synonyms:ykmA
Ordered Locus Names:BSU13150
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15C → G or S: Full repressor activity, but no modulation by peroxide. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543761 – 147Organic hydroperoxide resistance transcriptional regulatorAdd BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki15 ↔ 16N,N-(cysteine-1,S-diyl)phenylalanine (Cys-Phe); alternate
Modified residuei15Cysteine sulfenic acid (-SOH); alternate1 Publication1
Modified residuei15S-bacillithiol cysteine disulfide; alternate1
Modified residuei15S-cysteinyl cysteine; alternate1

Post-translational modificationi

Cys-15 is oxidized by organic peroxides to cysteine sulfenic acid (Cys-SOH). This can react with the alpha-amido of the following residue to form the sulfenamide cross-link. Oxidation or cross-linking results in the loss of DNA-binding activity and the inactivation of repressor function. Both the cysteine sulfenic acid and the sulfenamide cross-link can react with free cysteine or bacillithiol to form mixed disulfides. Further reduction of OhrR by free sulfhydryl compounds restores repressor activity.1 Publication

Keywords - PTMi

Oxidation

Proteomic databases

PaxDbiO34777.
PRIDEiO34777.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007281.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 12Combined sources3
Helixi14 – 26Combined sources13
Helixi30 – 33Combined sources4
Turni34 – 36Combined sources3
Helixi40 – 52Combined sources13
Beta strandi53 – 56Combined sources4
Helixi57 – 62Combined sources6
Turni63 – 65Combined sources3
Helixi68 – 81Combined sources14
Beta strandi83 – 85Combined sources3
Beta strandi95 – 100Combined sources6
Helixi102 – 105Combined sources4
Helixi107 – 110Combined sources4
Turni111 – 113Combined sources3
Helixi114 – 121Combined sources8
Helixi127 – 142Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z91X-ray2.50A1-147[»]
1Z9CX-ray2.64A/B/C/D/E/F1-147[»]
ProteinModelPortaliO34777.
SMRiO34777.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34777.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 141HTH marR-typePROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Contains 1 HTH marR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105K56. Bacteria.
ENOG4111TPH. LUCA.
HOGENOMiHOG000221448.
InParanoidiO34777.
OMAiMTKQYKP.
PhylomeDBiO34777.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000835. HTH_MarR-typ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01047. MarR. 1 hit.
[Graphical view]
PRINTSiPR00598. HTHMARR.
SMARTiSM00347. HTH_MARR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50995. HTH_MARR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENKFDHMKL ENQLCFLLYA SSREMTKQYK PLLDKLNITY PQYLALLLLW
60 70 80 90 100
EHETLTVKKM GEQLYLDSGT LTPMLKRMEQ QGLITRKRSE EDERSVLISL
110 120 130 140
TEDGALLKEK AVDIPGTILG LSKQSGEDLK QLKSALYTLL ETLHQKN
Length:147
Mass (Da):17,003
Last modified:January 1, 1998 - v1
Checksum:i4F277EA9AB5EE861
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA. Translation: CAA05594.1.
AL009126 Genomic DNA. Translation: CAB13172.1.
PIRiE69857.
RefSeqiNP_389198.1. NC_000964.3.
WP_003245653.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13172; CAB13172; BSU13150.
GeneIDi939850.
KEGGibsu:BSU13150.
PATRICi18974387. VBIBacSub10457_1386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA. Translation: CAA05594.1.
AL009126 Genomic DNA. Translation: CAB13172.1.
PIRiE69857.
RefSeqiNP_389198.1. NC_000964.3.
WP_003245653.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z91X-ray2.50A1-147[»]
1Z9CX-ray2.64A/B/C/D/E/F1-147[»]
ProteinModelPortaliO34777.
SMRiO34777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007281.

Proteomic databases

PaxDbiO34777.
PRIDEiO34777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13172; CAB13172; BSU13150.
GeneIDi939850.
KEGGibsu:BSU13150.
PATRICi18974387. VBIBacSub10457_1386.

Phylogenomic databases

eggNOGiENOG4105K56. Bacteria.
ENOG4111TPH. LUCA.
HOGENOMiHOG000221448.
InParanoidiO34777.
OMAiMTKQYKP.
PhylomeDBiO34777.

Enzyme and pathway databases

BioCyciBSUB:BSU13150-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO34777.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000835. HTH_MarR-typ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01047. MarR. 1 hit.
[Graphical view]
PRINTSiPR00598. HTHMARR.
SMARTiSM00347. HTH_MARR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50995. HTH_MARR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOHRR_BACSU
AccessioniPrimary (citable) accession number: O34777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.