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Protein

Sensor histidine kinase DesK

Gene

desK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DesR/DesK, responsible for cold induction of the des gene coding for the Delta5 acyl-lipid desaturase. Acts as a sensor of the membrane fluidity. Probably activates DesR by phosphorylation.5 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU19190-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase DesK (EC:2.7.13.3)
Gene namesi
Name:desK
Synonyms:yocF
Ordered Locus Names:BSU19190
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10ExtracellularSequence analysis10
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Topological domaini32 – 36CytoplasmicSequence analysis5
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 70ExtracellularSequence analysisAdd BLAST13
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Topological domaini92 – 103CytoplasmicSequence analysisAdd BLAST12
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 128ExtracellularSequence analysis4
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 370CytoplasmicSequence analysisAdd BLAST221

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003607801 – 370Sensor histidine kinase DesKAdd BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Phosphohistidine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO34757.

Interactioni

Protein-protein interaction databases

DIPiDIP-48966N.
STRINGi224308.Bsubs1_010100010591.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi157 – 162Combined sources6
Helixi171 – 208Combined sources38
Turni209 – 211Combined sources3
Helixi213 – 235Combined sources23
Turni236 – 241Combined sources6
Helixi246 – 259Combined sources14
Beta strandi263 – 265Combined sources3
Helixi277 – 297Combined sources21
Beta strandi301 – 310Combined sources10
Beta strandi313 – 322Combined sources10
Turni328 – 330Combined sources3
Helixi336 – 346Combined sources11
Beta strandi350 – 354Combined sources5
Beta strandi356 – 366Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EHFX-ray3.10A/B/C/D175-370[»]
3EHGX-ray1.74A243-370[»]
3EHHX-ray2.10A/B154-370[»]
3EHJX-ray2.50A/B154-370[»]
3GIEX-ray2.65A/B154-370[»]
3GIFX-ray2.70A/B154-370[»]
3GIGX-ray3.50A/B154-370[»]
ProteinModelPortaliO34757.
SMRiO34757.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34757.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini186 – 369Histidine kinaseAdd BLAST184

Sequence similaritiesi

Contains 1 histidine kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ZGX. Bacteria.
COG4585. LUCA.
HOGENOMiHOG000019645.
InParanoidiO34757.
KOiK07778.
OMAiWTIFFIL.
PhylomeDBiO34757.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF07730. HisKA_3. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.

Sequencei

Sequence statusi: Complete.

O34757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNHFTFQK LNGITPYIWT IFFILPFYFI WKSSSTFVII VGIILTLLFF
60 70 80 90 100
SVYRFAFVSK GWTIYLWGFL LIGISTASIT LFSYIYFAFF IAYFIGNIKE
110 120 130 140 150
RVPFHILYYV HLISAAVAAN FSLVLKKEFF LTQIPFVVIT LISAILLPFS
160 170 180 190 200
IKSRKERERL EEKLEDANER IAELVKLEER QRIARDLHDT LGQKLSLIGL
210 220 230 240 250
KSDLARKLIY KDPEQAAREL KSVQQTARTS LNEVRKIVSS MKGIRLKDEL
260 270 280 290 300
INIKQILEAA DIMFIYEEEK WPENISLLNE NILSMCLKEA VTNVVKHSQA
310 320 330 340 350
KTCRVDIQQL WKEVVITVSD DGTFKGEENS FSKGHGLLGM RERLEFANGS
360 370
LHIDTENGTK LTMAIPNNSK
Length:370
Mass (Da):42,673
Last modified:January 1, 1998 - v1
Checksum:i3A22FD6A3630A13D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027868 Genomic DNA. Translation: AAB84437.1.
AL009126 Genomic DNA. Translation: CAB13811.1.
PIRiC69901.
RefSeqiNP_389800.1. NC_000964.3.
WP_003231271.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13811; CAB13811; BSU19190.
GeneIDi939678.
KEGGibsu:BSU19190.
PATRICi18975687. VBIBacSub10457_2034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027868 Genomic DNA. Translation: AAB84437.1.
AL009126 Genomic DNA. Translation: CAB13811.1.
PIRiC69901.
RefSeqiNP_389800.1. NC_000964.3.
WP_003231271.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EHFX-ray3.10A/B/C/D175-370[»]
3EHGX-ray1.74A243-370[»]
3EHHX-ray2.10A/B154-370[»]
3EHJX-ray2.50A/B154-370[»]
3GIEX-ray2.65A/B154-370[»]
3GIFX-ray2.70A/B154-370[»]
3GIGX-ray3.50A/B154-370[»]
ProteinModelPortaliO34757.
SMRiO34757.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48966N.
STRINGi224308.Bsubs1_010100010591.

Proteomic databases

PaxDbiO34757.

Protocols and materials databases

DNASUi939678.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13811; CAB13811; BSU19190.
GeneIDi939678.
KEGGibsu:BSU19190.
PATRICi18975687. VBIBacSub10457_2034.

Phylogenomic databases

eggNOGiENOG4108ZGX. Bacteria.
COG4585. LUCA.
HOGENOMiHOG000019645.
InParanoidiO34757.
KOiK07778.
OMAiWTIFFIL.
PhylomeDBiO34757.

Enzyme and pathway databases

BioCyciBSUB:BSU19190-MONOMER.
BRENDAi2.7.13.3. 658.

Miscellaneous databases

EvolutionaryTraceiO34757.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF07730. HisKA_3. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDESK_BACSU
AccessioniPrimary (citable) accession number: O34757
Secondary accession number(s): Q796C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.