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Protein

Sensor histidine kinase DesK

Gene

desK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DesR/DesK, responsible for cold induction of the des gene coding for the Delta5 acyl-lipid desaturase. Acts as a sensor of the membrane fluidity. Probably activates DesR by phosphorylation.5 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU19190-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase DesK (EC:2.7.13.3)
Gene namesi
Name:desK
Synonyms:yocF
Ordered Locus Names:BSU19190
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010ExtracellularSequence analysis
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Topological domaini32 – 365CytoplasmicSequence analysis
Transmembranei37 – 5721HelicalSequence analysisAdd
BLAST
Topological domaini58 – 7013ExtracellularSequence analysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence analysisAdd
BLAST
Topological domaini92 – 10312CytoplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 1284ExtracellularSequence analysis
Transmembranei129 – 14921HelicalSequence analysisAdd
BLAST
Topological domaini150 – 370221CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Sensor histidine kinase DesKPRO_0000360780Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881Phosphohistidine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO34757.

Interactioni

Protein-protein interaction databases

DIPiDIP-48966N.
STRINGi224308.Bsubs1_010100010591.

Structurei

Secondary structure

1
370
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi157 – 1626Combined sources
Helixi171 – 20838Combined sources
Turni209 – 2113Combined sources
Helixi213 – 23523Combined sources
Turni236 – 2416Combined sources
Helixi246 – 25914Combined sources
Beta strandi263 – 2653Combined sources
Helixi277 – 29721Combined sources
Beta strandi301 – 31010Combined sources
Beta strandi313 – 32210Combined sources
Turni328 – 3303Combined sources
Helixi336 – 34611Combined sources
Beta strandi350 – 3545Combined sources
Beta strandi356 – 36611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EHFX-ray3.10A/B/C/D175-370[»]
3EHGX-ray1.74A243-370[»]
3EHHX-ray2.10A/B154-370[»]
3EHJX-ray2.50A/B154-370[»]
3GIEX-ray2.65A/B154-370[»]
3GIFX-ray2.70A/B154-370[»]
3GIGX-ray3.50A/B154-370[»]
ProteinModelPortaliO34757.
SMRiO34757. Positions 164-368.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34757.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini186 – 369184Histidine kinaseAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ZGX. Bacteria.
COG4585. LUCA.
HOGENOMiHOG000019645.
InParanoidiO34757.
KOiK07778.
OMAiWTIFFIL.
PhylomeDBiO34757.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF07730. HisKA_3. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.

Sequencei

Sequence statusi: Complete.

O34757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNHFTFQK LNGITPYIWT IFFILPFYFI WKSSSTFVII VGIILTLLFF
60 70 80 90 100
SVYRFAFVSK GWTIYLWGFL LIGISTASIT LFSYIYFAFF IAYFIGNIKE
110 120 130 140 150
RVPFHILYYV HLISAAVAAN FSLVLKKEFF LTQIPFVVIT LISAILLPFS
160 170 180 190 200
IKSRKERERL EEKLEDANER IAELVKLEER QRIARDLHDT LGQKLSLIGL
210 220 230 240 250
KSDLARKLIY KDPEQAAREL KSVQQTARTS LNEVRKIVSS MKGIRLKDEL
260 270 280 290 300
INIKQILEAA DIMFIYEEEK WPENISLLNE NILSMCLKEA VTNVVKHSQA
310 320 330 340 350
KTCRVDIQQL WKEVVITVSD DGTFKGEENS FSKGHGLLGM RERLEFANGS
360 370
LHIDTENGTK LTMAIPNNSK
Length:370
Mass (Da):42,673
Last modified:January 1, 1998 - v1
Checksum:i3A22FD6A3630A13D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027868 Genomic DNA. Translation: AAB84437.1.
AL009126 Genomic DNA. Translation: CAB13811.1.
PIRiC69901.
RefSeqiNP_389800.1. NC_000964.3.
WP_003231271.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13811; CAB13811; BSU19190.
GeneIDi939678.
KEGGibsu:BSU19190.
PATRICi18975687. VBIBacSub10457_2034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027868 Genomic DNA. Translation: AAB84437.1.
AL009126 Genomic DNA. Translation: CAB13811.1.
PIRiC69901.
RefSeqiNP_389800.1. NC_000964.3.
WP_003231271.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EHFX-ray3.10A/B/C/D175-370[»]
3EHGX-ray1.74A243-370[»]
3EHHX-ray2.10A/B154-370[»]
3EHJX-ray2.50A/B154-370[»]
3GIEX-ray2.65A/B154-370[»]
3GIFX-ray2.70A/B154-370[»]
3GIGX-ray3.50A/B154-370[»]
ProteinModelPortaliO34757.
SMRiO34757. Positions 164-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48966N.
STRINGi224308.Bsubs1_010100010591.

Proteomic databases

PaxDbiO34757.

Protocols and materials databases

DNASUi939678.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13811; CAB13811; BSU19190.
GeneIDi939678.
KEGGibsu:BSU19190.
PATRICi18975687. VBIBacSub10457_2034.

Phylogenomic databases

eggNOGiENOG4108ZGX. Bacteria.
COG4585. LUCA.
HOGENOMiHOG000019645.
InParanoidiO34757.
KOiK07778.
OMAiWTIFFIL.
PhylomeDBiO34757.

Enzyme and pathway databases

BioCyciBSUB:BSU19190-MONOMER.
BRENDAi2.7.13.3. 658.

Miscellaneous databases

EvolutionaryTraceiO34757.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF07730. HisKA_3. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDESK_BACSU
AccessioniPrimary (citable) accession number: O34757
Secondary accession number(s): Q796C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.