O34750 (YFML_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ATP-dependent RNA helicase yfmL
EC=3.6.4.13

Gene names

Name:	yfmL
Ordered Locus Names:	BSU07430

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Putative ATP-dependent RNA helicase yfmL		PRO_0000360838
Regions
	Domain	35 – 205	171	Helicase ATP-binding
	Domain	231 – 374	144	Helicase C-terminal
	Nucleotide binding	48 – 55	8	ATP By similarity
	Motif	153 – 156	4	DEAD box

Sequences

	Sequence		Length	Mass (Da)
	O34750 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6D5E4B6FAA4723E6	FASTA	376	42,189
	10 20 30 40 50 60 MTQTWPFLHN AQSFIQENWN ASGFQKPTPV QEQAAQLIMD GKDVIAESPT GTGKTLAYAL 70 80 90 100 110 120 PVLERIKPEQ KHPQAVILAP SRELVMQIFQ VIQDWKAGSE LRAASLIGGA NVKKQVEKLK 130 140 150 160 170 180 KHPHIIVGTP GRVFELIKAK KLKMHEVKTI VLDETDQLVL PEHRETMKQI IKTTLRDRQL 190 200 210 220 230 240 LCFSATLKKE TEDVLRELAQ EPEVLKVQRS KAEAGKVKHQ YLICDQRDKV KLLQKLSRLE 250 260 270 280 290 300 GMQALVFVRD IGNLSVYAEK LAYHHVELGV LHSEAKKMER AKIIATFEDG EFPLLLATDI 310 320 330 340 350 360 AARGLDIENL PYVIHADIPD EDGYVHRSGR TGRAGKEGNV LSLVTKLEES KLKKMAKKLG 370 VELSEAVYAG GKLKTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein." Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J. Gene 194:191-199(1997) [PubMed: 9272861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / AC327.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86417 Genomic DNA. Translation: BAA22326.1. AL009126 Genomic DNA. Translation: CAB12572.1.
PIR	C69813.
RefSeq	NP_388624.1. NC_000964.3.
3D structure databases
HSSP	HSSP built from PDB template 1QDE based on UniProtKB P10081.
ProteinModelPortal	O34750.
SMR	O34750. Positions 4-367.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003074; EBBACP00000003074; EBBACG00000003068.
GeneID	938792.
GenomeReviews	Gene locus BSU07430 in contig AL009126_GR.
KEGG	bsu:BSU07430.
NMPDR	fig|224308.1.peg.743.
PATRIC	18973138. VBIBacSub10457_0783.
Organism-specific databases
GenoList	BSU07430.
Phylogenomic databases
GeneTree	EBGT00070000031730.
HOGENOM	HBG737336.
OMA	SATRIAN.
PhylomeDB	O34750.
ProtClustDB	CLSK886848.
Enzyme and pathway databases
BioCyc	BSUB:BSU07430-MONOMER.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YFML_BACSU

Accession

Primary (citable) accession number: O34750
Secondary accession number(s): Q79ES5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program