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Protein

Probable ATP-dependent RNA helicase YfmL

Gene

yfmL

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A probable DEAD-box RNA helicase that plays a role in ribosomal 50S subunit assembly. May be a non-specific RNA helicase.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 55ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU07430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase YfmL (EC:3.6.4.13)
Gene namesi
Name:yfmL
Ordered Locus Names:BSU07430
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

No visible effect at 37 degrees Celsius, decreased growth at 16 degrees Celsius. A quadruple disruption of all RNA helicases (cshA, cshB, deaD, yfmL) is not lethal at 37 degrees Celsius, although both 50S and 70S ribosomes are decreased, while growth stops at 16 degrees.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003608381 – 376Probable ATP-dependent RNA helicase YfmLAdd BLAST376

Proteomic databases

PaxDbiO34750.
PRIDEiO34750.

Expressioni

Inductioni

In rich medium highest expression in exponential growth, expression decreases in stationary phase (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100004163.

Structurei

3D structure databases

ProteinModelPortaliO34750.
SMRiO34750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 205Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini231 – 374Helicase C-terminalPROSITE-ProRule annotationAdd BLAST144

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi153 – 156DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated

Phylogenomic databases

eggNOGiENOG4105FDU. Bacteria.
COG0513. LUCA.
HOGENOMiHOG000268811.
InParanoidiO34750.
OMAiFTHRNGR.
PhylomeDBiO34750.

Family and domain databases

InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

O34750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTWPFLHN AQSFIQENWN ASGFQKPTPV QEQAAQLIMD GKDVIAESPT
60 70 80 90 100
GTGKTLAYAL PVLERIKPEQ KHPQAVILAP SRELVMQIFQ VIQDWKAGSE
110 120 130 140 150
LRAASLIGGA NVKKQVEKLK KHPHIIVGTP GRVFELIKAK KLKMHEVKTI
160 170 180 190 200
VLDETDQLVL PEHRETMKQI IKTTLRDRQL LCFSATLKKE TEDVLRELAQ
210 220 230 240 250
EPEVLKVQRS KAEAGKVKHQ YLICDQRDKV KLLQKLSRLE GMQALVFVRD
260 270 280 290 300
IGNLSVYAEK LAYHHVELGV LHSEAKKMER AKIIATFEDG EFPLLLATDI
310 320 330 340 350
AARGLDIENL PYVIHADIPD EDGYVHRSGR TGRAGKEGNV LSLVTKLEES
360 370
KLKKMAKKLG VELSEAVYAG GKLKTK
Length:376
Mass (Da):42,189
Last modified:January 1, 1998 - v1
Checksum:i6D5E4B6FAA4723E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86417 Genomic DNA. Translation: BAA22326.1.
AL009126 Genomic DNA. Translation: CAB12572.1.
PIRiC69813.
RefSeqiNP_388624.1. NC_000964.3.
WP_003233750.1. NZ_JNCM01000032.1.

Genome annotation databases

EnsemblBacteriaiCAB12572; CAB12572; BSU07430.
GeneIDi938792.
KEGGibsu:BSU07430.
PATRICifig|224308.179.peg.806.

Similar proteinsi

Entry informationi

Entry nameiYFML_BACSU
AccessioniPrimary (citable) accession number: O34750
Secondary accession number(s): Q79ES5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: July 5, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families