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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

sumT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.By similarity

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrinogen-III C-methyltransferase (sumT)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrinogen-III C-methyltransferase (sumT)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei117S-adenosyl-L-methionineBy similarity1
Binding sitei170S-adenosyl-L-methionine; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU15610-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:sumT
Synonyms:ylnD
Ordered Locus Names:BSU15610
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003784911 – 257Uroporphyrinogen-III C-methyltransferaseAdd BLAST257

Proteomic databases

PaxDbiO34744.

Expressioni

Inductioni

Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008626.

Structurei

3D structure databases

ProteinModelPortaliO34744.
SMRiO34744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 89S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105CB8. Bacteria.
COG0007. LUCA.
HOGENOMiHOG000290519.
InParanoidiO34744.
KOiK02303.
OMAiYMGIHNL.
PhylomeDBiO34744.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiView protein in InterPro
IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
PfamiView protein in Pfam
PF00590. TP_methylase. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiView protein in PROSITE
PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O34744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVYIVGAG PGDPDLLTIK ALKAIEKADV ILYDRLVNKE ILQYAKEQAD
60 70 80 90 100
LIYCGKLPDF HTMKQETINR FLVKYAQKGK MVVRLKGGDP FVFGRGGEEA
110 120 130 140 150
ECLSENGIPF EIIPGITSGI AAAAYAGIPV THRDAGSNVA FVTGHYKKEE
160 170 180 190 200
DFEEKWKALA TGIDTLVIYM GIKNVQQIER KLLENGRDGS TPAAFIHWGT
210 220 230 240 250
TDKQKSVFCT VDTLSETVIK ENITNPSLIV IGNVVNYHYK LEWFESELKK

QDLSEAL
Length:257
Mass (Da):28,516
Last modified:January 1, 1998 - v1
Checksum:iB1D28A495D27DA85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000974 Genomic DNA. Translation: CAA04413.1.
AL009126 Genomic DNA. Translation: CAB13435.1.
PIRiD69877.
RefSeqiNP_389444.1. NC_000964.3.
WP_003232095.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13435; CAB13435; BSU15610.
GeneIDi936712.
KEGGibsu:BSU15610.
PATRICifig|224308.179.peg.1701.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSUMT_BACSU
AccessioniPrimary (citable) accession number: O34744
Secondary accession number(s): Q796I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families