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O34744

- SUMT_BACSU

UniProt

O34744 - SUMT_BACSU

Protein

Uroporphyrinogen-III C-methyltransferase

Gene

sumT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei117 – 1171S-adenosyl-L-methionineBy similarity
    Binding sitei170 – 1701S-adenosyl-L-methionine; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. precorrin-2 dehydrogenase activity Source: InterPro
    2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
    2. siroheme biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBSUB:BSU15610-MONOMER.
    UniPathwayiUPA00148; UER00211.
    UPA00262; UER00211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
    Short name:
    Urogen III methylase
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name:
    UROM
    Gene namesi
    Name:sumT
    Synonyms:ylnD
    Ordered Locus Names:BSU15610
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU15610. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 257257Uroporphyrinogen-III C-methyltransferasePRO_0000378491Add
    BLAST

    Proteomic databases

    PaxDbiO34744.

    Expressioni

    Inductioni

    Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU15610.

    Structurei

    3D structure databases

    ProteinModelPortaliO34744.
    SMRiO34744. Positions 2-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Belongs to the precorrin methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0007.
    HOGENOMiHOG000290519.
    KOiK02303.
    OMAiPAAFIHW.
    OrthoDBiEOG6BGNXZ.
    PhylomeDBiO34744.

    Family and domain databases

    Gene3Di3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF00590. TP_methylase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O34744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKVYIVGAG PGDPDLLTIK ALKAIEKADV ILYDRLVNKE ILQYAKEQAD    50
    LIYCGKLPDF HTMKQETINR FLVKYAQKGK MVVRLKGGDP FVFGRGGEEA 100
    ECLSENGIPF EIIPGITSGI AAAAYAGIPV THRDAGSNVA FVTGHYKKEE 150
    DFEEKWKALA TGIDTLVIYM GIKNVQQIER KLLENGRDGS TPAAFIHWGT 200
    TDKQKSVFCT VDTLSETVIK ENITNPSLIV IGNVVNYHYK LEWFESELKK 250
    QDLSEAL 257
    Length:257
    Mass (Da):28,516
    Last modified:January 1, 1998 - v1
    Checksum:iB1D28A495D27DA85
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000974 Genomic DNA. Translation: CAA04413.1.
    AL009126 Genomic DNA. Translation: CAB13435.1.
    PIRiD69877.
    RefSeqiNP_389444.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13435; CAB13435; BSU15610.
    GeneIDi936712.
    KEGGibsu:BSU15610.
    PATRICi18974929. VBIBacSub10457_1656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000974 Genomic DNA. Translation: CAA04413.1 .
    AL009126 Genomic DNA. Translation: CAB13435.1 .
    PIRi D69877.
    RefSeqi NP_389444.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali O34744.
    SMRi O34744. Positions 2-247.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU15610.

    Proteomic databases

    PaxDbi O34744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13435 ; CAB13435 ; BSU15610 .
    GeneIDi 936712.
    KEGGi bsu:BSU15610.
    PATRICi 18974929. VBIBacSub10457_1656.

    Organism-specific databases

    GenoListi BSU15610. [Micado ]

    Phylogenomic databases

    eggNOGi COG0007.
    HOGENOMi HOG000290519.
    KOi K02303.
    OMAi PAAFIHW.
    OrthoDBi EOG6BGNXZ.
    PhylomeDBi O34744.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00262 ; UER00211 .
    BioCyci BSUB:BSU15610-MONOMER.

    Family and domain databases

    Gene3Di 3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing 8 Kbp of DNA from Bacillus subtilis downstream of the pyr operon."
      Foulger D., Errington J.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Transcriptional control of the sulfur-regulated cysH operon, containing genes involved in L-cysteine biosynthesis in Bacillus subtilis."
      Mansilla M.C., Albanesi D., de Mendoza D.
      J. Bacteriol. 182:5885-5892(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: 168 / JH642.

    Entry informationi

    Entry nameiSUMT_BACSU
    AccessioniPrimary (citable) accession number: O34744
    Secondary accession number(s): Q796I5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3