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O34744

- SUMT_BACSU

UniProt

O34744 - SUMT_BACSU

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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

sumT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.By similarity

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei117 – 1171S-adenosyl-L-methionineBy similarity
Binding sitei170 – 1701S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

  1. precorrin-2 dehydrogenase activity Source: InterPro
  2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. siroheme biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU15610-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:sumT
Synonyms:ylnD
Ordered Locus Names:BSU15610
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU15610. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Uroporphyrinogen-III C-methyltransferasePRO_0000378491Add
BLAST

Proteomic databases

PaxDbiO34744.

Expressioni

Inductioni

Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU15610.

Structurei

3D structure databases

ProteinModelPortaliO34744.
SMRiO34744. Positions 2-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 893S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290519.
InParanoidiO34744.
KOiK02303.
OMAiPAAFIHW.
OrthoDBiEOG6BGNXZ.
PhylomeDBiO34744.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34744-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKVYIVGAG PGDPDLLTIK ALKAIEKADV ILYDRLVNKE ILQYAKEQAD
60 70 80 90 100
LIYCGKLPDF HTMKQETINR FLVKYAQKGK MVVRLKGGDP FVFGRGGEEA
110 120 130 140 150
ECLSENGIPF EIIPGITSGI AAAAYAGIPV THRDAGSNVA FVTGHYKKEE
160 170 180 190 200
DFEEKWKALA TGIDTLVIYM GIKNVQQIER KLLENGRDGS TPAAFIHWGT
210 220 230 240 250
TDKQKSVFCT VDTLSETVIK ENITNPSLIV IGNVVNYHYK LEWFESELKK

QDLSEAL
Length:257
Mass (Da):28,516
Last modified:January 1, 1998 - v1
Checksum:iB1D28A495D27DA85
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000974 Genomic DNA. Translation: CAA04413.1.
AL009126 Genomic DNA. Translation: CAB13435.1.
PIRiD69877.
RefSeqiNP_389444.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13435; CAB13435; BSU15610.
GeneIDi936712.
KEGGibsu:BSU15610.
PATRICi18974929. VBIBacSub10457_1656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000974 Genomic DNA. Translation: CAA04413.1 .
AL009126 Genomic DNA. Translation: CAB13435.1 .
PIRi D69877.
RefSeqi NP_389444.1. NC_000964.3.

3D structure databases

ProteinModelPortali O34744.
SMRi O34744. Positions 2-247.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU15610.

Proteomic databases

PaxDbi O34744.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13435 ; CAB13435 ; BSU15610 .
GeneIDi 936712.
KEGGi bsu:BSU15610.
PATRICi 18974929. VBIBacSub10457_1656.

Organism-specific databases

GenoListi BSU15610. [Micado ]

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290519.
InParanoidi O34744.
KOi K02303.
OMAi PAAFIHW.
OrthoDBi EOG6BGNXZ.
PhylomeDBi O34744.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00262 ; UER00211 .
BioCyci BSUB:BSU15610-MONOMER.

Family and domain databases

Gene3Di 3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF00590. TP_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing 8 Kbp of DNA from Bacillus subtilis downstream of the pyr operon."
    Foulger D., Errington J.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Transcriptional control of the sulfur-regulated cysH operon, containing genes involved in L-cysteine biosynthesis in Bacillus subtilis."
    Mansilla M.C., Albanesi D., de Mendoza D.
    J. Bacteriol. 182:5885-5892(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 168 / JH642.

Entry informationi

Entry nameiSUMT_BACSU
AccessioniPrimary (citable) accession number: O34744
Secondary accession number(s): Q796I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3