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O34680 (YDIP_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable BsuMI modification methylase subunit YdiP

Short name=M2.BsuMI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase M2.BsuMI
Gene names
Name:ydiP
Ordered Locus Names:BSU06070
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This methylase may recognize the double-stranded sequence CTCGAG, causing specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsuMI endonuclease. Ref.3 Ref.5

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subunit structure

May form a complex with YdiO, also seems to be active alone.

Developmental stage

Not expressed during sporulation. Ref.5

Induction

Constitutively expressed during exponential growth. Encoded in an operon with ydiO and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible. Ref.5

Disruption phenotype

Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025) has been disrupted. Ref.5

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Caution

The characterized enzyme (Ref.3) was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Probable BsuMI modification methylase subunit YdiP
PRO_0000379885

Regions

Domain1 – 299299SAM-dependent MTase C5-type

Sites

Active site691 By similarity

Sequences

Sequence LengthMass (Da)Tools
O34680 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 16578833225AFE22

FASTA38943,284
        10         20         30         40         50         60 
MKVVSLFSGI GGIELGLHQS GHTTEIFCEV DPLAKAVLSK NFPGVKIEDD INEIRELPSC 

        70         80         90        100        110        120 
DLVAAGFPCQ DLSQAGGKEG IDGSRSGLVK KLFELIEKKE HANRPPWILI ENVPYMLRLN 

       130        140        150        160        170        180 
RGKAMSYLTS VLSELGYTWA YRTVDARCFG LPQRRHRVIL LASLFEDPKD VIFSQDHSEP 

       190        200        210        220        230        240 
DLDGKPSVVD HSNYYGFYWT EGLRGVGWAR EAVPPIKCGS SVGIASPPAV WSPYEDIVGT 

       250        260        270        280        290        300 
INIRDAERLQ GFPEDWTNIT TETGKDIKEG ARWRLVGNAV SVRVSKWIGE NLSQPKGSIS 

       310        320        330        340        350        360 
DFEGELVTKT WPSAAWGYGD KKYKVPVSKW VANTEQIAIS EFLNHPLKPL SARALNGFLG 

       370        380 
RAARCTNVNY SDEFINSLER CKDRQLQKV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"DNA methyltransferase of Bacillus subtilis Marburg: purification, properties and further evidence of specificity."
Guha S.
Gene 74:77-81(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
Bron S., Janniere L., Ehrlich S.D.
Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA TARGET SEQUENCE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[5]"Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
Ohshima H., Matsuoka S., Asai K., Sadaie Y.
J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007637 Genomic DNA. Translation: BAA22751.1.
AL009126 Genomic DNA. Translation: CAB12426.1.
PIRA69788.
RefSeqNP_388488.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34680.
SMRO34680. Positions 1-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU06070.

Protein family/group databases

REBASE3613. M2.BsuMI.

Proteomic databases

PaxDbO34680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12426; CAB12426; BSU06070.
GeneID939876.
KEGGbsu:BSU06070.
PATRIC18972846. VBIBacSub10457_0637.

Organism-specific databases

GenoListBSU06070.

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000226162.
KOK00558.
OMAVAAGFPC.
OrthoDBEOG6Z3KHQ.
PhylomeDBO34680.

Enzyme and pathway databases

BioCycBSUB:BSU06070-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 2 hits.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYDIP_BACSU
AccessionPrimary (citable) accession number: O34680
Secondary accession number(s): Q797D0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList