Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O34667

- LUXS_BACSU

UniProt

O34667 - LUXS_BACSU

Protein

S-ribosylhomocysteine lyase

Gene

luxS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).

    Catalytic activityi

    S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione.

    Cofactori

    Binds 1 iron ion per subunit.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Iron
    Metal bindingi58 – 581Iron
    Metal bindingi126 – 1261Iron

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. S-ribosylhomocysteine lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. quorum sensing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Autoinducer synthesis, Quorum sensing

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU30670-MONOMER.
    MetaCyc:MONOMER-14557.
    BRENDAi4.4.1.21. 700.
    SABIO-RKO34667.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-ribosylhomocysteine lyase (EC:4.4.1.21)
    Alternative name(s):
    AI-2 synthesis protein
    Autoinducer-2 production protein LuxS
    Gene namesi
    Name:luxS
    Synonyms:ytjB
    Ordered Locus Names:BSU30670
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU30670. [Micado]

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi57 – 571E → A or Q: Complete loss of activity. 1 Publication
    Mutagenesisi57 – 571E → D: 220-fold decrease in activity. 1 Publication
    Mutagenesisi84 – 841C → A: Complete loss of activity. 1 Publication
    Mutagenesisi84 – 841C → D or S: Almost complete loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157S-ribosylhomocysteine lyasePRO_0000172211Add
    BLAST

    Proteomic databases

    PaxDbiO34667.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi224308.BSU30670.

    Structurei

    Secondary structure

    1
    157
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 84
    Turni11 – 133
    Beta strandi16 – 2712
    Turni28 – 303
    Beta strandi31 – 399
    Turni43 – 453
    Helixi50 – 6819
    Beta strandi72 – 8110
    Beta strandi85 – 9410
    Helixi98 – 11215
    Turni123 – 1253
    Turni127 – 1304
    Helixi134 – 14512
    Helixi149 – 1524

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IE0X-ray1.60A1-157[»]
    1J98X-ray1.20A1-157[»]
    1JQWX-ray2.30A1-157[»]
    1JVIX-ray2.20A1-157[»]
    1YCLX-ray1.80A2-157[»]
    2FQOX-ray1.87A1-157[»]
    2FQTX-ray1.79A1-157[»]
    ProteinModelPortaliO34667.
    SMRiO34667. Positions 4-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO34667.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LuxS family.Curated

    Phylogenomic databases

    eggNOGiCOG1854.
    HOGENOMiHOG000040372.
    KOiK07173.
    OMAiEFRAVIW.
    OrthoDBiEOG68WRBM.
    PhylomeDBiO34667.

    Family and domain databases

    Gene3Di3.30.1360.80. 1 hit.
    HAMAPiMF_00091. LuxS.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR003815. S-ribosylhomocysteinase.
    [Graphical view]
    PfamiPF02664. LuxS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006160. AI2. 1 hit.
    PRINTSiPR01487. LUXSPROTEIN.
    ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF63411. SSF63411. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O34667-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSVESFELD HNAVVAPYVR HCGVHKVGTD GVVNKFDIRF CQPNKQAMKP    50
    DTIHTLEHLL AFTIRSHAEK YDHFDIIDIS PMGCQTGYYL VVSGEPTSAE 100
    IVDLLEDTMK EAVEITEIPA ANEKQCGQAK LHDLEGAKRL MRFWLSQDKE 150
    ELLKVFG 157
    Length:157
    Mass (Da):17,714
    Last modified:January 1, 1998 - v1
    Checksum:iDD011A2A88BD2FFB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008220 Genomic DNA. Translation: AAC00235.1.
    AL009126 Genomic DNA. Translation: CAB15045.1.
    PIRiA69994.
    RefSeqiNP_390945.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15045; CAB15045; BSU30670.
    GeneIDi937106.
    KEGGibsu:BSU30670.
    PATRICi18978038. VBIBacSub10457_3207.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008220 Genomic DNA. Translation: AAC00235.1 .
    AL009126 Genomic DNA. Translation: CAB15045.1 .
    PIRi A69994.
    RefSeqi NP_390945.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IE0 X-ray 1.60 A 1-157 [» ]
    1J98 X-ray 1.20 A 1-157 [» ]
    1JQW X-ray 2.30 A 1-157 [» ]
    1JVI X-ray 2.20 A 1-157 [» ]
    1YCL X-ray 1.80 A 2-157 [» ]
    2FQO X-ray 1.87 A 1-157 [» ]
    2FQT X-ray 1.79 A 1-157 [» ]
    ProteinModelPortali O34667.
    SMRi O34667. Positions 4-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU30670.

    Chemistry

    BindingDBi O34667.
    ChEMBLi CHEMBL5171.

    Proteomic databases

    PaxDbi O34667.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15045 ; CAB15045 ; BSU30670 .
    GeneIDi 937106.
    KEGGi bsu:BSU30670.
    PATRICi 18978038. VBIBacSub10457_3207.

    Organism-specific databases

    GenoListi BSU30670. [Micado ]

    Phylogenomic databases

    eggNOGi COG1854.
    HOGENOMi HOG000040372.
    KOi K07173.
    OMAi EFRAVIW.
    OrthoDBi EOG68WRBM.
    PhylomeDBi O34667.

    Enzyme and pathway databases

    BioCyci BSUB:BSU30670-MONOMER.
    MetaCyc:MONOMER-14557.
    BRENDAi 4.4.1.21. 700.
    SABIO-RK O34667.

    Miscellaneous databases

    EvolutionaryTracei O34667.
    PROi O34667.

    Family and domain databases

    Gene3Di 3.30.1360.80. 1 hit.
    HAMAPi MF_00091. LuxS.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR003815. S-ribosylhomocysteinase.
    [Graphical view ]
    Pfami PF02664. LuxS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006160. AI2. 1 hit.
    PRINTSi PR01487. LUXSPROTEIN.
    ProDomi PD013172. S-ribosylhomocysteinase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF63411. SSF63411. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
      Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
      Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "S-ribosylhomocysteinase (LuxS) is a mononuclear iron protein."
      Zhu J., Dizin E., Hu X., Wavreille A.-S., Park J., Pei D.
      Biochemistry 42:4717-4726(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR, CHARACTERIZATION, MUTAGENESIS OF GLU-57 AND CYS-84.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS).
    5. "Crystal structure of the quorum-sensing protein LuxS reveals a catalytic metal site."
      Hilgers M.T., Ludwig M.L.
      Proc. Natl. Acad. Sci. U.S.A. 98:11169-11174(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiLUXS_BACSU
    AccessioniPrimary (citable) accession number: O34667
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3