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Protein

S-ribosylhomocysteine lyase

Gene

luxS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).

Catalytic activityi

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione.

Cofactori

Fe cation1 PublicationNote: Binds 1 Fe cation per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Iron1
Metal bindingi58Iron1
Metal bindingi126Iron1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Autoinducer synthesis, Quorum sensing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU30670-MONOMER.
MetaCyc:MONOMER-14557.
BRENDAi4.4.1.21. 658.
SABIO-RKO34667.

Names & Taxonomyi

Protein namesi
Recommended name:
S-ribosylhomocysteine lyase (EC:4.4.1.21)
Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS
Gene namesi
Name:luxS
Synonyms:ytjB
Ordered Locus Names:BSU30670
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57E → A or Q: Complete loss of activity. 1 Publication1
Mutagenesisi57E → D: 220-fold decrease in activity. 1 Publication1
Mutagenesisi84C → A: Complete loss of activity. 1 Publication1
Mutagenesisi84C → D or S: Almost complete loss of activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5171.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001722111 – 157S-ribosylhomocysteine lyaseAdd BLAST157

Proteomic databases

PaxDbiO34667.
PRIDEiO34667.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016691.

Chemistry databases

BindingDBiO34667.

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Turni11 – 13Combined sources3
Beta strandi16 – 27Combined sources12
Turni28 – 30Combined sources3
Beta strandi31 – 39Combined sources9
Turni43 – 45Combined sources3
Helixi50 – 68Combined sources19
Beta strandi72 – 81Combined sources10
Beta strandi85 – 94Combined sources10
Helixi98 – 112Combined sources15
Turni123 – 125Combined sources3
Turni127 – 130Combined sources4
Helixi134 – 145Combined sources12
Helixi149 – 152Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IE0X-ray1.60A1-157[»]
1J98X-ray1.20A1-157[»]
1JQWX-ray2.30A1-157[»]
1JVIX-ray2.20A1-157[»]
1YCLX-ray1.80A2-157[»]
2FQOX-ray1.87A1-157[»]
2FQTX-ray1.79A1-157[»]
ProteinModelPortaliO34667.
SMRiO34667.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34667.

Family & Domainsi

Sequence similaritiesi

Belongs to the LuxS family.Curated

Phylogenomic databases

eggNOGiENOG4106762. Bacteria.
COG1854. LUCA.
HOGENOMiHOG000040372.
InParanoidiO34667.
KOiK07173.
OMAiAGFMREH.
PhylomeDBiO34667.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
PfamiPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF63411. SSF63411. 1 hit.

Sequencei

Sequence statusi: Complete.

O34667-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSVESFELD HNAVVAPYVR HCGVHKVGTD GVVNKFDIRF CQPNKQAMKP
60 70 80 90 100
DTIHTLEHLL AFTIRSHAEK YDHFDIIDIS PMGCQTGYYL VVSGEPTSAE
110 120 130 140 150
IVDLLEDTMK EAVEITEIPA ANEKQCGQAK LHDLEGAKRL MRFWLSQDKE

ELLKVFG
Length:157
Mass (Da):17,714
Last modified:January 1, 1998 - v1
Checksum:iDD011A2A88BD2FFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00235.1.
AL009126 Genomic DNA. Translation: CAB15045.1.
PIRiA69994.
RefSeqiNP_390945.1. NC_000964.3.
WP_003219361.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB15045; CAB15045; BSU30670.
GeneIDi937106.
KEGGibsu:BSU30670.
PATRICi18978038. VBIBacSub10457_3207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00235.1.
AL009126 Genomic DNA. Translation: CAB15045.1.
PIRiA69994.
RefSeqiNP_390945.1. NC_000964.3.
WP_003219361.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IE0X-ray1.60A1-157[»]
1J98X-ray1.20A1-157[»]
1JQWX-ray2.30A1-157[»]
1JVIX-ray2.20A1-157[»]
1YCLX-ray1.80A2-157[»]
2FQOX-ray1.87A1-157[»]
2FQTX-ray1.79A1-157[»]
ProteinModelPortaliO34667.
SMRiO34667.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016691.

Chemistry databases

BindingDBiO34667.
ChEMBLiCHEMBL5171.

Proteomic databases

PaxDbiO34667.
PRIDEiO34667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15045; CAB15045; BSU30670.
GeneIDi937106.
KEGGibsu:BSU30670.
PATRICi18978038. VBIBacSub10457_3207.

Phylogenomic databases

eggNOGiENOG4106762. Bacteria.
COG1854. LUCA.
HOGENOMiHOG000040372.
InParanoidiO34667.
KOiK07173.
OMAiAGFMREH.
PhylomeDBiO34667.

Enzyme and pathway databases

BioCyciBSUB:BSU30670-MONOMER.
MetaCyc:MONOMER-14557.
BRENDAi4.4.1.21. 658.
SABIO-RKO34667.

Miscellaneous databases

EvolutionaryTraceiO34667.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
PfamiPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF63411. SSF63411. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLUXS_BACSU
AccessioniPrimary (citable) accession number: O34667
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.