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O34645 (AGAL_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-galactosidase
EC=3.2.1.22
Alternative name(s):
Melibiase
Gene names
Name:melA
Ordered Locus Names:BSU30300
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Cofactor

Binds 1 manganese ion per subunit By similarity.

Binds 1 NAD per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Alpha-galactosidase
PRO_0000169851

Regions

Nucleotide binding2 – 6867NAD By similarity

Sites

Active site1701Proton donor By similarity
Metal binding1691Manganese By similarity
Metal binding1991Manganese By similarity
Binding site1481Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O34645 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: E773A01018E65562

FASTA43249,264
        10         20         30         40         50         60 
MKKITFIGAG STIFAKNVLG DCLLTEALNG FEFALYDIDP KRLQESQLML ENLRDRYNPS 

        70         80         90        100        110        120 
VAINSYDDRK LALQNAGYVI NAIQVGGYKP STVIDFEIPK RYGLRQTIAD TVGIGGIFRS 

       130        140        150        160        170        180 
LRTIPVLFDI AKDMEEMCPD AWFLNYTNPM ATLTGAMLRY TNIKTIGLCH SVQVCTKDLF 

       190        200        210        220        230        240 
KALGMEHDGI EERIAGINHM AWLLEVKKDG TDLYPEIKRR AKEKQKTKHH DMVRFELMDK 

       250        260        270        280        290        300 
FGYYVTESSE HNAEYHPYFI KRNYPELISE LQIPLDEYPR RCVKQIENWE KMRDDIVNNK 

       310        320        330        340        350        360 
NLTHERSKEY GSRIIEAMET NEPFTFGGNV LNTGLITNLP SKAVVEVTCV ADRKKITPCF 

       370        380        390        400        410        420 
AGELPEQLAA LNRTNINTQL MTIEAAVTRK KEAVYQAAML DPHTSAELSM KDIISMCDDL 

       430 
FAAHGDWLPE YK

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF008220 Genomic DNA. Translation: AAC00383.1.
AL009126 Genomic DNA. Translation: CAB15008.1.
PIRE69656.
RefSeqNP_390908.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34645.
SMRO34645. Positions 3-421.
ModBaseSearch...

Protein family/group databases

CAZyGH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003353; EBBACP00000003353; EBBACG00000003346.
GeneID937255.
GenomeReviewsGene locus BSU30300 in contig AL009126_GR.
KEGGbsu:BSU30300.
NMPDRfig|224308.1.peg.3033.
PATRIC18977962. VBIBacSub10457_3169.

Organism-specific databases

GenoListBSU30300. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000000927.
HOGENOMHBG511715.
OMAMAINTWA.
PhylomeDBO34645.
ProtClustDBPRK15076.

Enzyme and pathway databases

BioCycBSUB:BSU30300-MONOMER.

Family and domain databases

InterProIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK07406.
PfamPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSPR00732. GLHYDRLASE4.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
PROSITEPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGAL_BACSU
AccessionPrimary (citable) accession number: O34645
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents