Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Blue-light photoreceptor

Gene

pfyP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits the same spectroscopical features and blue-light induced photochemistry as plants phototropins, with the reversible formation of a blue-shifted photoproduct, assigned to an FMN-cysteine thiol adduct. Although it is a positive regulator in the activation of the environmental signaling branch of the general stress transcription factor sigma-B, its precise role is undetermined.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Photoreceptor protein, Receptor

Keywords - Biological processi

Sensory transduction, Transcription, Transcription regulation

Keywords - Ligandi

Chromophore, Flavoprotein, FMN

Enzyme and pathway databases

BioCyciBSUB:BSU30340-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Blue-light photoreceptor
Alternative name(s):
Photoactive flavo-yellow protein
Phototropin homolog
Gene namesi
Name:pfyP
Synonyms:ytvA
Ordered Locus Names:BSU30340
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene have a twofold decreased response to salt and ethanol stress, and a somewhat reduced response to energy stress, indicating that PhoT is a positive regulator of sigma-B activity. It acts independently of both RsbRA and RsbRB (YkoB).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Blue-light photoreceptorPRO_0000172004Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621S-4a-FMN cysteine

Post-translational modificationi

FMN binds covalently to cysteine after exposure to blue light and this bond is spontaneously broken in the dark.

Proteomic databases

PaxDbiO34627.
PRIDEiO34627.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016521.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 73Combined sources
Helixi9 – 2012Combined sources
Beta strandi26 – 305Combined sources
Beta strandi39 – 424Combined sources
Helixi44 – 507Combined sources
Helixi54 – 574Combined sources
Helixi62 – 654Combined sources
Helixi72 – 8413Combined sources
Beta strandi88 – 958Combined sources
Beta strandi101 – 11313Combined sources
Beta strandi116 – 12510Combined sources
Helixi127 – 14519Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IUMmodel-A25-126[»]
2MWGNMR-A/B2-261[»]
2PR5X-ray1.45A/B20-147[»]
2PR6X-ray1.95A/B20-147[»]
4GCZX-ray2.30A/B1-126[»]
ProteinModelPortaliO34627.
SMRiO34627. Positions 20-147, 156-259.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34627.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 8776PASPROSITE-ProRule annotationAdd
BLAST
Domaini88 – 13851PACPROSITE-ProRule annotationAdd
BLAST
Domaini147 – 258112STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation
Contains 1 STAS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000262783.
InParanoidiO34627.
OMAiPIRNGIS.
PhylomeDBiO34627.

Family and domain databases

Gene3Di3.30.750.24. 1 hit.
InterProiIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR002645. STAS_dom.
[Graphical view]
PfamiPF13426. PAS_9. 1 hit.
PF01740. STAS. 1 hit.
[Graphical view]
SMARTiSM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFQSFGIP GQLEVIKKAL DHVRVGVVIT DPALEDNPIV YVNQGFVQMT
60 70 80 90 100
GYETEEILGK NCRFLQGKHT DPAEVDNIRT ALQNKEPVTV QIQNYKKDGT
110 120 130 140 150
MFWNELNIDP MEIEDKTYFV GIQNDITKQK EYEKLLEDSL TEITALSTPI
160 170 180 190 200
VPIRNGISAL PLVGNLTEER FNSIVCTLTN ILSTSKDDYL IIDLSGLAQV
210 220 230 240 250
NEQTADQIFK LSHLLKLTGT ELIITGIKPE LAMKMNKLDA NFSSLKTYSN
260
VKDAVKVLPI M
Length:261
Mass (Da):29,195
Last modified:January 1, 1998 - v1
Checksum:i4AD6EB00B6BD27DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00382.1.
AL009126 Genomic DNA. Translation: CAB15012.1.
PIRiA70002.
RefSeqiNP_390912.1. NC_000964.3.
WP_004399022.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB15012; CAB15012; BSU30340.
GeneIDi937254.
KEGGibsu:BSU30340.
PATRICi18977970. VBIBacSub10457_3173.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00382.1.
AL009126 Genomic DNA. Translation: CAB15012.1.
PIRiA70002.
RefSeqiNP_390912.1. NC_000964.3.
WP_004399022.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IUMmodel-A25-126[»]
2MWGNMR-A/B2-261[»]
2PR5X-ray1.45A/B20-147[»]
2PR6X-ray1.95A/B20-147[»]
4GCZX-ray2.30A/B1-126[»]
ProteinModelPortaliO34627.
SMRiO34627. Positions 20-147, 156-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016521.

Proteomic databases

PaxDbiO34627.
PRIDEiO34627.

Protocols and materials databases

DNASUi937254.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15012; CAB15012; BSU30340.
GeneIDi937254.
KEGGibsu:BSU30340.
PATRICi18977970. VBIBacSub10457_3173.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000262783.
InParanoidiO34627.
OMAiPIRNGIS.
PhylomeDBiO34627.

Enzyme and pathway databases

BioCyciBSUB:BSU30340-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO34627.

Family and domain databases

Gene3Di3.30.750.24. 1 hit.
InterProiIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR002645. STAS_dom.
[Graphical view]
PfamiPF13426. PAS_9. 1 hit.
PF01740. STAS. 1 hit.
[Graphical view]
SMARTiSM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHOT_BACSU
AccessioniPrimary (citable) accession number: O34627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.