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O34623 (DPO3A_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7
Gene names
Name:dnaE
Ordered Locus Names:BSU29230
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length1115 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5' exonuclease activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11151115DNA polymerase III subunit alpha
PRO_0000103310

Sequences

Sequence LengthMass (Da)Tools
O34623 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: E8B04E3398E512FE

FASTA1,115125,350
        10         20         30         40         50         60 
MSFVHLQVHS GYSLLNSAAA VEELVSEADR LGYASLALTD DHVMYGAIQF YKACKARGIN 

        70         80         90        100        110        120 
PIIGLTASVF TDDSELEAYP LVLLAKSNTG YQNLLKISSV LQSKSKGGLK PKWLHSYREG 

       130        140        150        160        170        180 
IIAITPGEKG YIETLLEGGL FEQAAQASLE FQSIFGKGAF YFSYQPFKGN QVLSEQILKL 

       190        200        210        220        230        240 
SEETGIPVTA TGDVHYIRKE DKAAYRCLKA IKAGEKLTDA PAEDLPDLDL KPLEEMQNIY 

       250        260        270        280        290        300 
REHPEALQAS VEIAEQCRVD VSLGQTRLPS FPTPDGTSAD DYLTDICMEG LRSRFGKPDE 

       310        320        330        340        350        360 
RYLRRLQYEL DVIKRMKFSD YFLIVWDFMK HAHEKGIVTG PGRGSAAGSL VAYVLYITDV 

       370        380        390        400        410        420 
DPIKHHLLFE RFLNPERVSM PDIDIDFPDT RRDEVIQYVQ QKYGAMHVAQ IITFGTLAAK 

       430        440        450        460        470        480 
AALRDVGRVF GVSPKEADQL AKLIPSRPGM TLDEARQQSP QLDKRLRESS LLQQVYSIAR 

       490        500        510        520        530        540 
KIEGLPRHAS THAAGVVLSE EPLTDVVPLQ EGHEGIYLTQ YAMDHLEDLG LLKMDFLGLR 

       550        560        570        580        590        600 
NLTLIESITS MIEKEENIKI DLSSISYSDD KTFSLLSKGD TTGIFQLESA GMRSVLKRLK 

       610        620        630        640        650        660 
PSGLEDIVAV NALYRPGPME NIPLFIDRKH GRAPVHYPHE DLRSILEDTY GVIVYQEQIM 

       670        680        690        700        710        720 
MIASRMAGFS LGEADLLRRA VSKKKKEILD RERSHFVEGC LKKEYSVDTA NEVYDLIVKF 

       730        740        750        760        770        780 
ANYGFNRSHA VAYSMIGCQL AYLKAHYPLY FMCGLLTSVI GNEDKISQYL YEAKGSGIRI 

       790        800        810        820        830        840 
LPPSVNKSSF PFTVENGSVR YSLRAIKSVG VSAVKDIYKA RKEKPFEDLF DFCFRVPSKS 

       850        860        870        880        890        900 
VNRKMLEALI FSGAMDEFGQ NRATLLASID VALEHAELFA ADDDQMGLFL DESFSIKPKY 

       910        920        930        940        950        960 
VETEELPLVD LLAFEKETLG IYFSNHPLSA FRKQLTAQGA VSILQAQRAV KRQLSLGVLL 

       970        980        990       1000       1010       1020 
SKIKTIRTKT GQNMAFLTLS DETGEMEAVV FPEQFRQLSP VLREGALLFT AGKCEVRQDK 

      1030       1040       1050       1060       1070       1080 
IQFIMSRAEL LEDMDAEKAP SVYIKIESSQ HSQEILAKIK RILLEHKGET GVYLYYERQK 

      1090       1100       1110 
QTIKLPESFH INADHQVLYR LKELLGQKNV VLKQW

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF008220 Genomic DNA. Translation: AAC00338.1.
AL009126 Genomic DNA. Translation: CAB14883.1.
PIRD69617.
RefSeqNP_390801.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34623.
ModBaseSearch...

Protein-protein interaction databases

IntActO34623. 7 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003677; EBBACP00000003677; EBBACG00000003670.
GeneID936593.
GenomeReviewsGene locus BSU29230 in contig AL009126_GR.
KEGGbsu:BSU29230.
NMPDRfig|224308.1.peg.2926.
PATRIC18977740. VBIBacSub10457_3058.

Organism-specific databases

GenoListBSU29230. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000032109.
HOGENOMHBG734490.
OMARVLQMPY.
PhylomeDBO34623.
ProtClustDBPRK06920.

Enzyme and pathway databases

BioCycBSUB:BSU29230-MONOMER.

Family and domain databases

InterProIPR011708. DNA_pol3_alpha.
IPR004365. NA-bd_OB_tRNA-helicase.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
KOK02337.
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. PHP-like. 1 hit.
TIGRFAMsTIGR00594. Polc. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3A_BACSU
AccessionPrimary (citable) accession number: O34623
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents