Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase PcrA

Gene

pcrA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA helicase used for plasmid rolling-circle replication and also involved in UV repair.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 39ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU06610-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PcrA (EC:3.6.4.12)
Gene namesi
Name:pcrA
Synonyms:yerF
Ordered Locus Names:BSU06610
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020511 – 739ATP-dependent DNA helicase PcrAAdd BLAST739

Proteomic databases

PaxDbiO34580
PRIDEiO34580

Interactioni

Subunit structurei

Interacts with YxaL; YwhK and YerB.1 Publication

Protein-protein interaction databases

IntActiO34580, 5 interactors
STRINGi224308.Bsubs1_010100003738

Structurei

3D structure databases

ProteinModelPortaliO34580
SMRiO34580
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 289UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST280
Domaini290 – 570UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST281

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C4R Bacteria
COG0210 LUCA
HOGENOMiHOG000033015
InParanoidiO34580
KOiK03657
OMAiYQDTNRT
PhylomeDBiO34580

Family and domain databases

Gene3Di1.10.10.160, 1 hit
InterProiView protein in InterPro
IPR005751 ATP-dep_DNA_helicase_PcrA
IPR013986 DExx_box_DNA_helicase_dom_sf
IPR014017 DNA_helicase_UvrD-like_C
IPR000212 DNA_helicase_UvrD/REP
IPR027417 P-loop_NTPase
IPR014016 UvrD-like_ATP-bd
IPR034739 UvrD/AddA_N
PANTHERiPTHR11070 PTHR11070, 1 hit
PfamiView protein in Pfam
PF00580 UvrD-helicase, 1 hit
PF13361 UvrD_C, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01073 pcrA, 1 hit
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
PS51217 UVRD_HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

O34580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYISNQLLS GLNPVQQEAV KTTDGPLLLM AGAGSGKTRV LTHRIAYLMA
60 70 80 90 100
EKHVAPWNIL AITFTNKAAR EMKERVESIL GPGADDIWIS TFHSMCVRIL
110 120 130 140 150
RRDIDRIGIN RNFSILDTAD QLSVIKGILK ERNLDPKKFD PRSILGTISS
160 170 180 190 200
AKNELTEPEE FSKVAGGYYD QVVSDVYADY QKKLLKNQSL DFDDLIMTTI
210 220 230 240 250
KLFDRVPEVL EFYQRKFQYI HVDEYQDTNR AQYMLVKQLA ERFQNLCVVG
260 270 280 290 300
DSDQSIYRWR GADITNILSF EKDYPNASVI LLEQNYRSTK RILRAANEVI
310 320 330 340 350
KNNSNRKPKN LWTENDEGIK ISYYRGDNEF GEGQFVAGKI HQLHSTGKRK
360 370 380 390 400
LSDIAILYRT NAQSRVIEET LLKAGLNYNI VGGTKFYDRK EIKDILAYLR
410 420 430 440 450
LVSNPDDDIS FTRIVNVPKR GVGATSLEKI ASYAAINGLS FFQAIQQVDF
460 470 480 490 500
IGVSAKAANA LDSFRQMIEN LTNMQDYLSI TELTEEILDK TEYREMLKAE
510 520 530 540 550
KSIEAQSRLE NIDEFLSVTK NFEQKSEDKT LVAFLTDLAL IADIDQLDQK
560 570 580 590 600
EEESGGKDAI TLMTLHAAKG LEFPVVFLMG LEEGVFPHSR SLMEEAEMEE
610 620 630 640 650
ERRLAYVGIT RAEQELYLTN AKMRTLFGRT NMNPESRFIA EIPDDLLENL
660 670 680 690 700
NEKKETRATS ARKMQPRRGP VSRPVSYASK TGGDTLNWAV GDKAGHKKWG
710 720 730
TGTVVSVKGE GEGTELDIAF PSPVGVKRLL AAFAPIEKQ
Length:739
Mass (Da):83,555
Last modified:January 1, 1998 - v1
Checksum:i70C553EFC88E6D6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15254 Genomic DNA Translation: CAA75552.1
AL009126 Genomic DNA Translation: CAB12481.1
PIRiE69794
RefSeqiNP_388543.1, NC_000964.3
WP_003233919.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12481; CAB12481; BSU06610
GeneIDi938747
KEGGibsu:BSU06610
PATRICifig|224308.179.peg.719

Entry informationi

Entry nameiPCRA_BACSU
AccessioniPrimary (citable) accession number: O34580
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health