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Protein

ATP-dependent DNA helicase PcrA

Gene

pcrA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase used for plasmid rolling-circle replication and also involved in UV repair.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei287 – 2871ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 396ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU06610-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PcrA (EC:3.6.4.12)
Gene namesi
Name:pcrA
Synonyms:yerF
Ordered Locus Names:BSU06610
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 739739ATP-dependent DNA helicase PcrAPRO_0000102051Add
BLAST

Proteomic databases

PaxDbiO34580.

Interactioni

Subunit structurei

Interacts with YxaL; YwhK and YerB.1 Publication

Protein-protein interaction databases

IntActiO34580. 5 interactions.
STRINGi224308.Bsubs1_010100003738.

Structurei

3D structure databases

ProteinModelPortaliO34580.
SMRiO34580. Positions 1-653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 289280UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini290 – 570281UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C4R. Bacteria.
COG0210. LUCA.
HOGENOMiHOG000033015.
InParanoidiO34580.
KOiK03657.
OMAiRLQIAFQ.
OrthoDBiEOG64N9TW.
PhylomeDBiO34580.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01073. pcrA. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYISNQLLS GLNPVQQEAV KTTDGPLLLM AGAGSGKTRV LTHRIAYLMA
60 70 80 90 100
EKHVAPWNIL AITFTNKAAR EMKERVESIL GPGADDIWIS TFHSMCVRIL
110 120 130 140 150
RRDIDRIGIN RNFSILDTAD QLSVIKGILK ERNLDPKKFD PRSILGTISS
160 170 180 190 200
AKNELTEPEE FSKVAGGYYD QVVSDVYADY QKKLLKNQSL DFDDLIMTTI
210 220 230 240 250
KLFDRVPEVL EFYQRKFQYI HVDEYQDTNR AQYMLVKQLA ERFQNLCVVG
260 270 280 290 300
DSDQSIYRWR GADITNILSF EKDYPNASVI LLEQNYRSTK RILRAANEVI
310 320 330 340 350
KNNSNRKPKN LWTENDEGIK ISYYRGDNEF GEGQFVAGKI HQLHSTGKRK
360 370 380 390 400
LSDIAILYRT NAQSRVIEET LLKAGLNYNI VGGTKFYDRK EIKDILAYLR
410 420 430 440 450
LVSNPDDDIS FTRIVNVPKR GVGATSLEKI ASYAAINGLS FFQAIQQVDF
460 470 480 490 500
IGVSAKAANA LDSFRQMIEN LTNMQDYLSI TELTEEILDK TEYREMLKAE
510 520 530 540 550
KSIEAQSRLE NIDEFLSVTK NFEQKSEDKT LVAFLTDLAL IADIDQLDQK
560 570 580 590 600
EEESGGKDAI TLMTLHAAKG LEFPVVFLMG LEEGVFPHSR SLMEEAEMEE
610 620 630 640 650
ERRLAYVGIT RAEQELYLTN AKMRTLFGRT NMNPESRFIA EIPDDLLENL
660 670 680 690 700
NEKKETRATS ARKMQPRRGP VSRPVSYASK TGGDTLNWAV GDKAGHKKWG
710 720 730
TGTVVSVKGE GEGTELDIAF PSPVGVKRLL AAFAPIEKQ
Length:739
Mass (Da):83,555
Last modified:January 1, 1998 - v1
Checksum:i70C553EFC88E6D6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15254 Genomic DNA. Translation: CAA75552.1.
AL009126 Genomic DNA. Translation: CAB12481.1.
PIRiE69794.
RefSeqiNP_388543.1. NC_000964.3.
WP_003233919.1. NZ_JNCM01000032.1.

Genome annotation databases

EnsemblBacteriaiCAB12481; CAB12481; BSU06610.
GeneIDi938747.
KEGGibsu:BSU06610.
PATRICi18972968. VBIBacSub10457_0698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15254 Genomic DNA. Translation: CAA75552.1.
AL009126 Genomic DNA. Translation: CAB12481.1.
PIRiE69794.
RefSeqiNP_388543.1. NC_000964.3.
WP_003233919.1. NZ_JNCM01000032.1.

3D structure databases

ProteinModelPortaliO34580.
SMRiO34580. Positions 1-653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34580. 5 interactions.
STRINGi224308.Bsubs1_010100003738.

Proteomic databases

PaxDbiO34580.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12481; CAB12481; BSU06610.
GeneIDi938747.
KEGGibsu:BSU06610.
PATRICi18972968. VBIBacSub10457_0698.

Phylogenomic databases

eggNOGiENOG4105C4R. Bacteria.
COG0210. LUCA.
HOGENOMiHOG000033015.
InParanoidiO34580.
KOiK03657.
OMAiRLQIAFQ.
OrthoDBiEOG64N9TW.
PhylomeDBiO34580.

Enzyme and pathway databases

BioCyciBSUB:BSU06610-MONOMER.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR005751. ATP-dep_DNA_helicase_PcrA.
IPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01073. pcrA. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication."
    Petit M.-A., Dervyn E., Rose M., Entian K.-D., McGovern S., Ehrlich S.D., Bruand C.
    Mol. Microbiol. 29:261-273(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "The beta-propeller protein YxaL increases the processivity of the PcrA helicase."
    Noirot-Gros M.-F., Soultanas P., Wigley D.B., Ehrlich S.D., Noirot P., Petit M.-A.
    Mol. Genet. Genomics 267:391-400(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YXAL; YWHK AND YERB.

Entry informationi

Entry nameiPCRA_BACSU
AccessioniPrimary (citable) accession number: O34580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.