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Protein

Putative ribosome biogenesis GTPase RsgA

Gene

rsgA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Depletion of yloQ results in sensitization towards protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, implying that YloQ functions in conjunction with the ribosome in vivo. Decreasing levels of YloQ lead to an increase in unassembled 30S and 50S subunit and a decrease in the assembled 70S ribosome.
May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (Potential). Dispensible for viability, but important for overall fitness (Probable).UniRule annotationCurated

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521ZincUniRule annotation1 Publication
Metal bindingi257 – 2571ZincUniRule annotation1 Publication
Metal bindingi259 – 2591ZincUniRule annotation1 Publication
Metal bindingi265 – 2651ZincUniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1194GTPUniRule annotation
Nucleotide bindingi171 – 1799GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU15780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ribosome biogenesis GTPase RsgAUniRule annotation (EC:3.6.1.-UniRule annotation)
Gene namesi
Name:rsgAUniRule annotation
Synonyms:engC, yloQ
Ordered Locus Names:BSU15780
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells yloQ have a slow growth phenotype and grow as chains of filaments. A cell curvature phenotype is also present, resulting in long wavy cells or short curved rods.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Putative ribosome biogenesis GTPase RsgAPRO_0000171464Add
BLAST

Proteomic databases

PaxDbiO34530.

Interactioni

Subunit structurei

Monomer. Associates with ribosomes.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008711.

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Beta strandi13 – 186Combined sources
Beta strandi20 – 234Combined sources
Beta strandi25 – 317Combined sources
Beta strandi47 – 515Combined sources
Beta strandi58 – 636Combined sources
Turni71 – 744Combined sources
Beta strandi80 – 878Combined sources
Turni88 – 914Combined sources
Helixi94 – 10512Combined sources
Turni106 – 1083Combined sources
Beta strandi110 – 1167Combined sources
Helixi118 – 1203Combined sources
Helixi124 – 14017Combined sources
Beta strandi144 – 1463Combined sources
Helixi149 – 1524Combined sources
Turni156 – 1583Combined sources
Helixi159 – 1624Combined sources
Beta strandi165 – 1728Combined sources
Helixi173 – 18412Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi217 – 2226Combined sources
Helixi235 – 2384Combined sources
Helixi239 – 2413Combined sources
Helixi243 – 2486Combined sources
Helixi249 – 2513Combined sources
Beta strandi259 – 2613Combined sources
Helixi266 – 2727Combined sources
Helixi278 – 29215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T9HX-ray1.60A1-298[»]
ProteinModelPortaliO34530.
SMRiO34530. Positions 1-298.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34530.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 228162CP-type GPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi252 – 26514Knuckle-like cysteine clusterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily.UniRule annotationCurated
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105E06. Bacteria.
COG1162. LUCA.
HOGENOMiHOG000006958.
InParanoidiO34530.
KOiK06949.
OMAiTHIIAAN.
PhylomeDBiO34530.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01820. GTPase_RsgA. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004881. Ribosome_biogen_GTPase_RsgA.
IPR010914. RsgA_GTPase_dom.
IPR031944. RsgA_N.
[Graphical view]
PfamiPF03193. DUF258. 1 hit.
PF16745. RsgA_N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00157. TIGR00157. 1 hit.
PROSITEiPS50936. ENGC_GTPASE. 1 hit.
PS51721. G_CP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGKIIKAL SGFYYVLDES EDSDKVIQCR GRGIFRKNKI TPLVGDYVVY
60 70 80 90 100
QAENDKEGYL MEIKERTNEL IRPPICNVDQ AVLVFSAVQP SFSTALLDRF
110 120 130 140 150
LVLVEANDIQ PIICITKMDL IEDQDTEDTI QAYAEDYRNI GYDVYLTSSK
160 170 180 190 200
DQDSLADIIP HFQDKTTVFA GQSGVGKSSL LNAISPELGL RTNEISEHLG
210 220 230 240 250
RGKHTTRHVE LIHTSGGLVA DTPGFSSLEF TDIEEEELGY TFPDIREKSS
260 270 280 290
SCKFRGCLHL KEPKCAVKQA VEDGELKQYR YDHYVEFMTE IKDRKPRY
Length:298
Mass (Da):33,797
Last modified:January 1, 1998 - v1
Checksum:iE58A69CAE570F855
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA. Translation: CAA74251.1.
AL009126 Genomic DNA. Translation: CAB13451.1.
PIRiA69879.
RefSeqiNP_389460.1. NC_000964.3.
WP_003232060.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13451; CAB13451; BSU15780.
GeneIDi938451.
KEGGibsu:BSU15780.
PATRICi18974963. VBIBacSub10457_1673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA. Translation: CAA74251.1.
AL009126 Genomic DNA. Translation: CAB13451.1.
PIRiA69879.
RefSeqiNP_389460.1. NC_000964.3.
WP_003232060.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T9HX-ray1.60A1-298[»]
ProteinModelPortaliO34530.
SMRiO34530. Positions 1-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008711.

Proteomic databases

PaxDbiO34530.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13451; CAB13451; BSU15780.
GeneIDi938451.
KEGGibsu:BSU15780.
PATRICi18974963. VBIBacSub10457_1673.

Phylogenomic databases

eggNOGiENOG4105E06. Bacteria.
COG1162. LUCA.
HOGENOMiHOG000006958.
InParanoidiO34530.
KOiK06949.
OMAiTHIIAAN.
PhylomeDBiO34530.

Enzyme and pathway databases

BioCyciBSUB:BSU15780-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO34530.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01820. GTPase_RsgA. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004881. Ribosome_biogen_GTPase_RsgA.
IPR010914. RsgA_GTPase_dom.
IPR031944. RsgA_N.
[Graphical view]
PfamiPF03193. DUF258. 1 hit.
PF16745. RsgA_N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00157. TIGR00157. 1 hit.
PROSITEiPS50936. ENGC_GTPASE. 1 hit.
PS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSGA_BACSU
AccessioniPrimary (citable) accession number: O34530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.