Reviewed,
UniProtKB/Swiss-Prot O34529 (K6PF_BACSU)
Last modified
February 9, 2010.
Version 75.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: 6-phosphofructokinase Short name=Phosphofructokinase EC=2.7.1.11 Alternative name(s): Phosphohexokinase | ||||||
| Gene names |
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| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 319 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339 |
| Pathway | Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339 |
| Subunit structure | Homotetramer By similarity. HAMAP MF_00339 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00339. |
| Sequence similarities | Belongs to the phosphofructokinase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | Allosteric enzyme Complete proteome |
| Gene Ontology (GO) | |
| Biological process | fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysisInferred from electronic annotation. Source: HAMAP |
| Cellular component | 6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | 6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP bindingInferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 319 | 319 | 6-phosphofructokinase HAMAP MF_00339 | PRO_0000111936 | |||||
Regions | |||||||||
| Nucleotide binding | 21 – 25 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 154 – 158 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 171 – 187 | 17 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 127 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 185 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Metal binding | 187 | 1 | Magnesium By similarity | ||||||
| Binding site | 162 | 1 | Substrate By similarity | ||||||
| Binding site | 243 | 1 | Substrate By similarity | ||||||
| Binding site | 249 | 1 | Substrate By similarity | ||||||
| Binding site | 252 | 1 | Substrate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF008220 Genomic DNA. Translation: AAC00342.1. AL009126 Genomic DNA. Translation: CAB14879.1. |
| PIR | A69675. |
| RefSeq | NP_390797.1. |
3D structure databases | |
| SMR | O34529. Positions 1-319. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 937376. |
| GenomeReviews | Gene locus BSU29190 in contig AL009126_GR. |
| KEGG | bsu:BSU29190. |
| NMPDR | fig|224308.1.peg.2922. |
Organism-specific databases | |
| SubtiList | BG12644. pfkA. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG405044. |
| OMA | DTCLNTV. |
| PhylomeDB | O34529. |
Enzyme and pathway databases | |
| BRENDA | 2.7.1.11. 150. |
Family and domain databases | |
| HAMAP | MF_00339. Phosphofructokinase. [Tree] |
| InterPro | IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view] |
| Pfam | PF00365. PFK. 1 hit. [Graphical view] |
| PIRSF | PIRSF000532. ATP_PFK_prok. 1 hit. |
| PRINTS | PR00476. PHFRCTKINASE. |
| TIGRFAMs | TIGR02482. PFKA_ATP. 1 hit. |
| PROSITE | PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | K6PF_BACSU | ||||||||
| Accession | Primary (citable) accession number: O34529 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
