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O34522

- TRMB_BACSU

UniProt

O34522 - TRMB_BACSU

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, ytmQ, BSU29900
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosyl-L-methionine By similarity
Binding sitei69 – 691S-adenosyl-L-methionine By similarity
Binding sitei96 – 961S-adenosyl-L-methionine By similarity
Active sitei118 – 1181 By similarity
Binding sitei118 – 1181S-adenosyl-L-methionine By similarity
Binding sitei122 – 1221Substrate By similarity
Binding sitei154 – 1541Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU29900-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
BsTrmB
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Synonyms:ytmQ
Ordered Locus Names:BSU29900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU29900. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_0000171295Add
BLAST

Proteomic databases

PaxDbiO34522.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU29900.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 156
Turni16 – 194
Helixi24 – 274
Helixi31 – 355
Beta strandi41 – 455
Helixi51 – 599
Beta strandi63 – 686
Helixi72 – 8413
Beta strandi88 – 936
Helixi97 – 993
Helixi100 – 1034
Beta strandi111 – 1166
Helixi123 – 1286
Helixi133 – 14311
Beta strandi148 – 1547
Helixi156 – 16914
Beta strandi172 – 1798
Helixi180 – 1823
Helixi194 – 1963
Beta strandi197 – 2015
Beta strandi205 – 2106

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FCAX-ray2.10A/B1-213[»]
ProteinModelPortaliO34522.
SMRiO34522. Positions 10-213.

Miscellaneous databases

EvolutionaryTraceiO34522.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1296Interaction with RNA Reviewed prediction
Regioni191 – 1944Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.
PhylomeDBiO34522.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34522-1 [UniParc]FASTAAdd to Basket

« Hide

MRMRHKPWAD DFLAENADIA ISNPADYKGK WNTVFGNDNP IHIEVGTGKG    50
QFISGMAKQN PDINYIGIEL FKSVIVTAVQ KVKDSEAQNV KLLNIDADTL 100
TDVFEPGEVK RVYLNFSDPW PKKRHEKRRL TYSHFLKKYE EVMGKGGSIH 150
FKTDNRGLFE YSLKSFSEYG LLLTYVSLDL HNSNLEGNIM TEYEEKFSAL 200
GQPIYRAEVE WRT 213
Length:213
Mass (Da):24,504
Last modified:January 1, 1998 - v1
Checksum:i28C34F163EB2DD69
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008220 Genomic DNA. Translation: AAC00285.1.
AL009126 Genomic DNA. Translation: CAB14968.1.
PIRiB69997.
RefSeqiNP_390868.1. NC_000964.3.
WP_004398646.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB14968; CAB14968; BSU29900.
GeneIDi936447.
KEGGibsu:BSU29900.
PATRICi18977886. VBIBacSub10457_3131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF008220 Genomic DNA. Translation: AAC00285.1 .
AL009126 Genomic DNA. Translation: CAB14968.1 .
PIRi B69997.
RefSeqi NP_390868.1. NC_000964.3.
WP_004398646.1. NZ_CM000487.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FCA X-ray 2.10 A/B 1-213 [» ]
ProteinModelPortali O34522.
SMRi O34522. Positions 10-213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU29900.

Proteomic databases

PaxDbi O34522.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14968 ; CAB14968 ; BSU29900 .
GeneIDi 936447.
KEGGi bsu:BSU29900.
PATRICi 18977886. VBIBacSub10457_3131.

Organism-specific databases

GenoListi BSU29900. [Micado ]

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.
PhylomeDBi O34522.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci BSUB:BSU29900-MONOMER.

Miscellaneous databases

EvolutionaryTracei O34522.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase."
    Zegers I., Gigot D., van Vliet F., Tricot C., Aymerich S., Bujnicki J.M., Kosinski J., Droogmans L.
    Nucleic Acids Res. 34:1925-1934(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), CATALYTIC ACTIVITY.
    Strain: 168.

Entry informationi

Entry nameiTRMB_BACSU
AccessioniPrimary (citable) accession number: O34522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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