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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation1 Publication

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosyl-L-methionineUniRule annotation1
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Active sitei118By similarity1
Binding sitei118S-adenosyl-L-methionineUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU29900-MONOMER
BRENDAi2.1.1.33 658
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation1 Publication)
Alternative name(s):
BsTrmB1 Publication
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferase1 PublicationUniRule annotation
Gene namesi
Name:trmB1 PublicationUniRule annotation
Synonyms:ytmQ
Ordered Locus Names:BSU29900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712951 – 213tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST213

Proteomic databases

PaxDbiO34522
PRIDEiO34522

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016306

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 15Combined sources6
Turni16 – 19Combined sources4
Helixi24 – 27Combined sources4
Helixi31 – 35Combined sources5
Beta strandi41 – 45Combined sources5
Helixi51 – 59Combined sources9
Beta strandi63 – 68Combined sources6
Helixi72 – 84Combined sources13
Beta strandi88 – 93Combined sources6
Helixi97 – 99Combined sources3
Helixi100 – 103Combined sources4
Beta strandi111 – 116Combined sources6
Helixi123 – 128Combined sources6
Helixi133 – 143Combined sources11
Beta strandi148 – 154Combined sources7
Helixi156 – 169Combined sources14
Beta strandi172 – 179Combined sources8
Helixi180 – 182Combined sources3
Helixi194 – 196Combined sources3
Beta strandi197 – 201Combined sources5
Beta strandi205 – 210Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FCAX-ray2.10A/B1-213[»]
ProteinModelPortaliO34522
SMRiO34522
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34522

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 129Interaction with RNAUniRule annotation6
Regioni191 – 194Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000251689
InParanoidiO34522
KOiK03439
OMAiWITFPDP
PhylomeDBiO34522

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

O34522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMRHKPWAD DFLAENADIA ISNPADYKGK WNTVFGNDNP IHIEVGTGKG
60 70 80 90 100
QFISGMAKQN PDINYIGIEL FKSVIVTAVQ KVKDSEAQNV KLLNIDADTL
110 120 130 140 150
TDVFEPGEVK RVYLNFSDPW PKKRHEKRRL TYSHFLKKYE EVMGKGGSIH
160 170 180 190 200
FKTDNRGLFE YSLKSFSEYG LLLTYVSLDL HNSNLEGNIM TEYEEKFSAL
210
GQPIYRAEVE WRT
Length:213
Mass (Da):24,504
Last modified:January 1, 1998 - v1
Checksum:i28C34F163EB2DD69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA Translation: AAC00285.1
AL009126 Genomic DNA Translation: CAB14968.1
PIRiB69997
RefSeqiNP_390868.1, NC_000964.3
WP_004398646.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14968; CAB14968; BSU29900
GeneIDi936447
KEGGibsu:BSU29900
PATRICifig|224308.179.peg.3248

Entry informationi

Entry nameiTRMB_BACSU
AccessioniPrimary (citable) accession number: O34522
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
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