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Protein

o-succinylbenzoate synthase

Gene

menC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB).UniRule annotation1 Publication

Catalytic activityi

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O.UniRule annotation1 Publication

Cofactori

a divalent metal cationUniRule annotationBy similarity

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotationBy similarity
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Isochorismate synthase MenF (menF)
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. 2-succinylbenzoate--CoA ligase (menE)
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotationBy similarity
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162Proton donorUniRule annotationBy similarity1
Metal bindingi189MagnesiumUniRule annotationBy similarity1
Metal bindingi214MagnesiumUniRule annotationBy similarity1
Metal bindingi239MagnesiumUniRule annotationBy similarity1
Active sitei263Proton acceptorUniRule annotationBy similarity1

GO - Molecular functioni

GO - Biological processi

  • menaquinone biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU30780-MONOMER.
MetaCyc:MONOMER-13796.
SABIO-RKO34514.
UniPathwayiUPA00079.
UPA01057; UER00165.

Names & Taxonomyi

Protein namesi
Recommended name:
o-succinylbenzoate synthase1 PublicationUniRule annotation (EC:4.2.1.113UniRule annotation1 Publication)
Short name:
OSB synthaseUniRule annotationCurated
Short name:
OSBSUniRule annotationCurated
Alternative name(s):
4-(2'-carboxyphenyl)-4-oxybutyric acid synthaseUniRule annotationCurated
o-succinylbenzoic acid synthaseUniRule annotationCurated
Gene namesi
Name:menCUniRule annotation
Synonyms:ytfD
Ordered Locus Names:BSU30780
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712821 – 371o-succinylbenzoate synthaseAdd BLAST371

Proteomic databases

PaxDbiO34514.
PRIDEiO34514.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016746.

Structurei

3D structure databases

ProteinModelPortaliO34514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DTQ. Bacteria.
COG4948. LUCA.
HOGENOMiHOG000185900.
InParanoidiO34514.
KOiK02549.
OMAiDDIVDHR.
PhylomeDBiO34514.

Family and domain databases

CDDicd03317. NAAAR. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01933. MenC_2. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
IPR010197. OSB_synthase_MenC_2.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01928. menC_lowGC/arch. 1 hit.

Sequencei

Sequence statusi: Complete.

O34514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIEKITLY HLSMNLKKPF KNSIETLQER KFLIVEAIDT SGVTGWGEVS
60 70 80 90 100
AFSSPWYTEE TIGTCLHMLK DFFIPNVVGR EFNHPSEVPD SLARYKGNRM
110 120 130 140 150
AKAGLESAVW DIYAKKKGVS LAEALGGTRD KVPAGVVVGL APLDDMLKEI
160 170 180 190 200
ESYQKEGYQR IKIKIQPGQD VELVKAIRSR FPTIPLMADA NSSYELKDIS
210 220 230 240 250
RLKELDDYHL MMIEQPLQAD DIVDHRHLQK HLKTAICLDE SICSVDDARR
260 270 280 290 300
AIELGSCKII NIKPSRVGGL TEALKIHDLC KEHHMQVWCG GMLETGISRA
310 320 330 340 350
QNVALASLPQ FTIPGDISSS SRYWDEDIVT PDIRIDNGFI SVSKQPGLGV
360 370
EVNQDIMRKY VTKMDVFTQH G
Length:371
Mass (Da):41,629
Last modified:January 1, 1998 - v1
Checksum:iDC6996175BDB5833
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00228.1.
AL009126 Genomic DNA. Translation: CAB15056.1.
PIRiF69991.
RefSeqiNP_390956.1. NC_000964.3.
WP_004398737.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB15056; CAB15056; BSU30780.
GeneIDi937201.
KEGGibsu:BSU30780.
PATRICi18978060. VBIBacSub10457_3218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00228.1.
AL009126 Genomic DNA. Translation: CAB15056.1.
PIRiF69991.
RefSeqiNP_390956.1. NC_000964.3.
WP_004398737.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO34514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016746.

Proteomic databases

PaxDbiO34514.
PRIDEiO34514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15056; CAB15056; BSU30780.
GeneIDi937201.
KEGGibsu:BSU30780.
PATRICi18978060. VBIBacSub10457_3218.

Phylogenomic databases

eggNOGiENOG4105DTQ. Bacteria.
COG4948. LUCA.
HOGENOMiHOG000185900.
InParanoidiO34514.
KOiK02549.
OMAiDDIVDHR.
PhylomeDBiO34514.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00165.
BioCyciBSUB:BSU30780-MONOMER.
MetaCyc:MONOMER-13796.
SABIO-RKO34514.

Family and domain databases

CDDicd03317. NAAAR. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01933. MenC_2. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
IPR010197. OSB_synthase_MenC_2.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF8. PTHR13794:SF8. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01928. menC_lowGC/arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMENC_BACSU
AccessioniPrimary (citable) accession number: O34514
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.