O34476 (CYSM_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	ytkP
Ordered Locus Names:	BSU29970

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Probable cysteine synthase		PRO_0000167082
Regions
	Region	182 – 186	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	75	1	Pyridoxal phosphate By similarity
	Binding site	270	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	45	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O34476 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1BBA91BC8218904F	FASTA	311	33,066
	10 20 30 40 50 60 MLKIVANTAD LIGDTPLVRL NRLQPENAAQ VYLKLEFFNP SGSVKDRAAY QMIIEAEQNG 70 80 90 100 110 120 LLKPGSVIIE PTSGNTGIGL AMNAAARGYK AILVMPDTMT KERINLLKAY GAEVVLTPGE 130 140 150 160 170 180 ERMPGSIKKA KELAEQIPNS YIPMQFDNTA NPDAHRKTTA PEIARAIEEI GKPLGAFVAS 190 200 210 220 230 240 SGTGGTITGT GEALKELFPD ITVHVVEPAG SPVLSGGKPG AHKLVGTSPG FIPPILNEDV 250 260 270 280 290 300 YDEIIKISDE DAYTTTRRLA AEEGILVGPS SGAACFAAIE TAKRLSPDKV VVCMTADTGE 310 RYLSTDLWSF I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008220 Genomic DNA. Translation: AAC00392.1. AL009126 Genomic DNA. Translation: CAB14975.1.
PIR	H69994.
RefSeq	NP_390875.1. NC_000964.3.
3D structure databases
ProteinModelPortal	O34476.
SMR	O34476. Positions 2-309.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000002566; EBBACP00000002566; EBBACG00000002561.
GeneID	936733.
GenomeReviews	Gene locus BSU29970 in contig AL009126_GR.
KEGG	bsu:BSU29970.
NMPDR	fig|224308.1.peg.3000.
PATRIC	18977900. VBIBacSub10457_3138.
Organism-specific databases
GenoList	BSU29970.
Phylogenomic databases
GeneTree	EBGT00070000031817.
HOGENOM	HBG748215.
OMA	SFIPMQF.
PhylomeDB	O34476.
ProtClustDB	CLSK873562.
Enzyme and pathway databases
BioCyc	BSUB:BSU29970-MONOMER.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_BACSU

Accession

Primary (citable) accession number: O34476

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program