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O34431

- ATCL_BACSU

UniProt

O34431 - ATCL_BACSU

Protein

Calcium-transporting ATPase

Gene

yloB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.1 Publication

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi279 – 2791Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi280 – 2801Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi282 – 2821Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi284 – 2841Calcium 2By similarity
    Active sitei326 – 32614-aspartylphosphate intermediateBy similarity
    Metal bindingi633 – 6331MagnesiumBy similarity
    Metal bindingi637 – 6371MagnesiumBy similarity
    Metal bindingi699 – 6991Calcium 1By similarity
    Metal bindingi702 – 7021Calcium 1By similarity
    Metal bindingi727 – 7271Calcium 2By similarity
    Metal bindingi730 – 7301Calcium 1By similarity
    Metal bindingi731 – 7311Calcium 1By similarity
    Metal bindingi731 – 7311Calcium 2By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium-transporting ATPase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU15650-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-transporting ATPase (EC:3.6.3.8)
    Alternative name(s):
    Calcium pump
    Gene namesi
    Name:yloB
    Ordered Locus Names:BSU15650
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU15650.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Spores are less resistant to heat and germinate at a slower rate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 890890Calcium-transporting ATPasePRO_0000360851Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO34431.

    Expressioni

    Developmental stagei

    Expressed from 4 hours and peaks at 9 hours after onset of sporulation.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU15650.

    Structurei

    3D structure databases

    ProteinModelPortaliO34431.
    SMRiO34431. Positions 2-890.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4747CytoplasmicBy similarityAdd
    BLAST
    Topological domaini69 – 7810ExtracellularBy similarity
    Topological domaini100 – 238139CytoplasmicBy similarityAdd
    BLAST
    Topological domaini259 – 27012ExtracellularBy similarityAdd
    BLAST
    Topological domaini289 – 688400CytoplasmicBy similarityAdd
    BLAST
    Topological domaini709 – 71810ExtracellularBy similarity
    Topological domaini740 – 75920CytoplasmicBy similarityAdd
    BLAST
    Topological domaini783 – 79816ExtracellularBy similarityAdd
    BLAST
    Topological domaini819 – 83012CytoplasmicBy similarityAdd
    BLAST
    Topological domaini850 – 86415ExtracellularBy similarityAdd
    BLAST
    Topological domaini886 – 8905CytoplasmicBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 6821Helical; Name=1By similarityAdd
    BLAST
    Transmembranei79 – 9921Helical; Name=2By similarityAdd
    BLAST
    Transmembranei239 – 25820Helical; Name=3By similarityAdd
    BLAST
    Transmembranei271 – 28818Helical; Name=4By similarityAdd
    BLAST
    Transmembranei689 – 70820Helical; Name=5By similarityAdd
    BLAST
    Transmembranei719 – 73921Helical; Name=6By similarityAdd
    BLAST
    Transmembranei760 – 78223Helical; Name=7By similarityAdd
    BLAST
    Transmembranei799 – 81820Helical; Name=8By similarityAdd
    BLAST
    Transmembranei831 – 84919Helical; Name=9By similarityAdd
    BLAST
    Transmembranei865 – 88521Helical; Name=10By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265621.
    KOiK01537.
    OMAiLWINLIL.
    OrthoDBiEOG6HF5WH.
    PhylomeDBiO34431.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR005782. ATPase_P-typ_Ca-transp_IIA.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O34431-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFHEMGQTD LLEATNTSMK QGLTEKEVKK RLDKHGPNEL QEGKKTSALL    50
    LFFAQFKDFM VLVLLAATLI SGFLGEYVDA VAIIAIVFVN GILGFFQERR 100
    AEQSLQALKE LSTPHVMALR EGSWTKIPSK ELVPGDIVKF TSGDRIGADV 150
    RIVEARSLEI EESALTGESI PVVKHADKLK KPDVSLGDIT NMAFMGTIVT 200
    RGSGVGVVVG TGMNTAMGKI ADMLESAGTL STPLQRRLEQ LGKILIVVAL 250
    LLTVLVVAVG VIQGHDLYSM FLAGVSLAVA AIPEGLPAIV TVALSLGVQR 300
    MIKQKSIVRK LPAVETLGCA SIICSDKTGT MTQNKMTVTH VWSGGKTWRV 350
    AGAGYEPKGS FTLNEKEISV NEHKPLQQML LFGALCNNSN IEKRDGEYVL 400
    DGDPTEGALL TAARKGGFSK EFVESNYRVI EEFPFDSARK MMTVIVENQD 450
    RKRYIITKGA PDVLMQRSSR IYYDGSAALF SNERKAETEA VLRHLASQAL 500
    RTIAVAYRPI KAGETPSMEQ AEKDLTMLGL SGIIDPPRPE VRQAIKECRE 550
    AGIKTVMITG DHVETAKAIA KDLRLLPKSG KIMDGKMLNE LSQEELSHVV 600
    EDVYVFARVS PEHKLKIVKA YQENGHIVAM TGDGVNDAPA IKQADIGVSM 650
    GITGTDVAKE ASSLVLVDDN FATIKSAIKE GRNIYENIRK FIRYLLASNV 700
    GEILVMLFAM LLALPLPLVP IQILWVNLVT DGLPAMALGM DQPEGDVMKR 750
    KPRHPKEGVF ARKLGWKVVS RGFLIGVATI LAFIIVYHRN PENLAYAQTI 800
    AFATLVLAQL IHVFDCRSET SVFSRNPFQN LYLIGAVLSS ILLMLVVIYY 850
    PPLQPIFHTV AITPGDWMLV IGMSAIPTFL LAGSLLTRKK 890
    Length:890
    Mass (Da):97,293
    Last modified:January 1, 1998 - v1
    Checksum:i43CB25F7A9BA31A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13937 Genomic DNA. Translation: CAA74269.1.
    AL009126 Genomic DNA. Translation: CAB13439.1.
    PIRiH69877.
    RefSeqiNP_389448.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13439; CAB13439; BSU15650.
    GeneIDi936954.
    KEGGibsu:BSU15650.
    PATRICi18974937. VBIBacSub10457_1660.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13937 Genomic DNA. Translation: CAA74269.1 .
    AL009126 Genomic DNA. Translation: CAB13439.1 .
    PIRi H69877.
    RefSeqi NP_389448.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali O34431.
    SMRi O34431. Positions 2-890.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU15650.

    Proteomic databases

    PaxDbi O34431.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13439 ; CAB13439 ; BSU15650 .
    GeneIDi 936954.
    KEGGi bsu:BSU15650.
    PATRICi 18974937. VBIBacSub10457_1660.

    Organism-specific databases

    GenoListi BSU15650.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265621.
    KOi K01537.
    OMAi LWINLIL.
    OrthoDBi EOG6HF5WH.
    PhylomeDBi O34431.

    Enzyme and pathway databases

    BioCyci BSUB:BSU15650-MONOMER.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR005782. ATPase_P-typ_Ca-transp_IIA.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01116. ATPase-IIA1_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 28 kbp segment from the spoVM region of the Bacillus subtilis 168 genome."
      Foulger D., Errington J.
      Microbiology 144:801-805(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Expression of a P-type Ca(2+)-transport ATPase in Bacillus subtilis during sporulation."
      Raeymaekers L., Wuytack E., Willems I., Michiels C.W., Wuytack F.
      Cell Calcium 32:93-93(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
      Strain: 168 / PS832.

    Entry informationi

    Entry nameiATCL_BACSU
    AccessioniPrimary (citable) accession number: O34431
    Secondary accession number(s): Q799L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3