Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-transporting ATPase

Gene

yloB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi279Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi280Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi282Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi284Calcium 2By similarity1
Active sitei3264-aspartylphosphate intermediateBy similarity1
Metal bindingi633MagnesiumBy similarity1
Metal bindingi637MagnesiumBy similarity1
Metal bindingi699Calcium 1By similarity1
Metal bindingi702Calcium 1By similarity1
Metal bindingi727Calcium 2By similarity1
Metal bindingi730Calcium 1By similarity1
Metal bindingi731Calcium 1By similarity1
Metal bindingi731Calcium 2By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15650-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase (EC:3.6.3.8)
Alternative name(s):
Calcium pump
Gene namesi
Name:yloB
Ordered Locus Names:BSU15650
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47CytoplasmicBy similarityAdd BLAST47
Transmembranei48 – 68Helical; Name=1By similarityAdd BLAST21
Topological domaini69 – 78ExtracellularBy similarity10
Transmembranei79 – 99Helical; Name=2By similarityAdd BLAST21
Topological domaini100 – 238CytoplasmicBy similarityAdd BLAST139
Transmembranei239 – 258Helical; Name=3By similarityAdd BLAST20
Topological domaini259 – 270ExtracellularBy similarityAdd BLAST12
Transmembranei271 – 288Helical; Name=4By similarityAdd BLAST18
Topological domaini289 – 688CytoplasmicBy similarityAdd BLAST400
Transmembranei689 – 708Helical; Name=5By similarityAdd BLAST20
Topological domaini709 – 718ExtracellularBy similarity10
Transmembranei719 – 739Helical; Name=6By similarityAdd BLAST21
Topological domaini740 – 759CytoplasmicBy similarityAdd BLAST20
Transmembranei760 – 782Helical; Name=7By similarityAdd BLAST23
Topological domaini783 – 798ExtracellularBy similarityAdd BLAST16
Transmembranei799 – 818Helical; Name=8By similarityAdd BLAST20
Topological domaini819 – 830CytoplasmicBy similarityAdd BLAST12
Transmembranei831 – 849Helical; Name=9By similarityAdd BLAST19
Topological domaini850 – 864ExtracellularBy similarityAdd BLAST15
Transmembranei865 – 885Helical; Name=10By similarityAdd BLAST21
Topological domaini886 – 890CytoplasmicBy similarity5

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Spores are less resistant to heat and germinate at a slower rate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003608511 – 890Calcium-transporting ATPaseAdd BLAST890

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO34431.

Expressioni

Developmental stagei

Expressed from 4 hours and peaks at 9 hours after onset of sporulation.1 Publication

Interactioni

Protein-protein interaction databases

IntActiO34431. 1 interactor.
STRINGi224308.Bsubs1_010100008646.

Structurei

3D structure databases

ProteinModelPortaliO34431.
SMRiO34431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiO34431.
KOiK01537.
OMAiIAIAYRP.
PhylomeDBiO34431.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFHEMGQTD LLEATNTSMK QGLTEKEVKK RLDKHGPNEL QEGKKTSALL
60 70 80 90 100
LFFAQFKDFM VLVLLAATLI SGFLGEYVDA VAIIAIVFVN GILGFFQERR
110 120 130 140 150
AEQSLQALKE LSTPHVMALR EGSWTKIPSK ELVPGDIVKF TSGDRIGADV
160 170 180 190 200
RIVEARSLEI EESALTGESI PVVKHADKLK KPDVSLGDIT NMAFMGTIVT
210 220 230 240 250
RGSGVGVVVG TGMNTAMGKI ADMLESAGTL STPLQRRLEQ LGKILIVVAL
260 270 280 290 300
LLTVLVVAVG VIQGHDLYSM FLAGVSLAVA AIPEGLPAIV TVALSLGVQR
310 320 330 340 350
MIKQKSIVRK LPAVETLGCA SIICSDKTGT MTQNKMTVTH VWSGGKTWRV
360 370 380 390 400
AGAGYEPKGS FTLNEKEISV NEHKPLQQML LFGALCNNSN IEKRDGEYVL
410 420 430 440 450
DGDPTEGALL TAARKGGFSK EFVESNYRVI EEFPFDSARK MMTVIVENQD
460 470 480 490 500
RKRYIITKGA PDVLMQRSSR IYYDGSAALF SNERKAETEA VLRHLASQAL
510 520 530 540 550
RTIAVAYRPI KAGETPSMEQ AEKDLTMLGL SGIIDPPRPE VRQAIKECRE
560 570 580 590 600
AGIKTVMITG DHVETAKAIA KDLRLLPKSG KIMDGKMLNE LSQEELSHVV
610 620 630 640 650
EDVYVFARVS PEHKLKIVKA YQENGHIVAM TGDGVNDAPA IKQADIGVSM
660 670 680 690 700
GITGTDVAKE ASSLVLVDDN FATIKSAIKE GRNIYENIRK FIRYLLASNV
710 720 730 740 750
GEILVMLFAM LLALPLPLVP IQILWVNLVT DGLPAMALGM DQPEGDVMKR
760 770 780 790 800
KPRHPKEGVF ARKLGWKVVS RGFLIGVATI LAFIIVYHRN PENLAYAQTI
810 820 830 840 850
AFATLVLAQL IHVFDCRSET SVFSRNPFQN LYLIGAVLSS ILLMLVVIYY
860 870 880 890
PPLQPIFHTV AITPGDWMLV IGMSAIPTFL LAGSLLTRKK
Length:890
Mass (Da):97,293
Last modified:January 1, 1998 - v1
Checksum:i43CB25F7A9BA31A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA. Translation: CAA74269.1.
AL009126 Genomic DNA. Translation: CAB13439.1.
PIRiH69877.
RefSeqiNP_389448.1. NC_000964.3.
WP_003232087.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13439; CAB13439; BSU15650.
GeneIDi936954.
KEGGibsu:BSU15650.
PATRICi18974937. VBIBacSub10457_1660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13937 Genomic DNA. Translation: CAA74269.1.
AL009126 Genomic DNA. Translation: CAB13439.1.
PIRiH69877.
RefSeqiNP_389448.1. NC_000964.3.
WP_003232087.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO34431.
SMRiO34431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34431. 1 interactor.
STRINGi224308.Bsubs1_010100008646.

Proteomic databases

PaxDbiO34431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13439; CAB13439; BSU15650.
GeneIDi936954.
KEGGibsu:BSU15650.
PATRICi18974937. VBIBacSub10457_1660.

Phylogenomic databases

eggNOGiCOG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiO34431.
KOiK01537.
OMAiIAIAYRP.
PhylomeDBiO34431.

Enzyme and pathway databases

BioCyciBSUB:BSU15650-MONOMER.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATCL_BACSU
AccessioniPrimary (citable) accession number: O34431
Secondary accession number(s): Q799L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.