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Protein

Sensor protein CitS

Gene

citS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg2+-citrate complex.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU07580-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein CitS (EC:2.7.13.3)
Gene namesi
Name:citS
Synonyms:yflR
Ordered Locus Names:BSU07580
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Topological domaini35 – 175141ExtracellularSequence analysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence analysisAdd
BLAST
Topological domaini197 – 542346CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Sensor protein CitSPRO_0000074735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei339 – 3391Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO34427.

Interactioni

Protein-protein interaction databases

IntActiO34427. 23 interactions.
STRINGi224308.Bsubs1_010100004233.

Structurei

3D structure databases

ProteinModelPortaliO34427.
SMRiO34427. Positions 38-162, 204-315, 416-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini216 – 27964PASAdd
BLAST
Domaini336 – 528193Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiO34427.
KOiK11637.
OMAiARTISYM.
PhylomeDBiO34427.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKRFHFSL QTKIMGLIAA LLVFVIGVLT ITLAVQHTQG ERRQAEQLAV
60 70 80 90 100
QTARTISYMP PVKELIERKD GHAAQTQEVI EQMKEQTGAF AIYVLNEKGD
110 120 130 140 150
IRSASGKSGL KKLERSREIL FGGSHVSETK ADGRRVIRGS APIIKEQKGY
160 170 180 190 200
SQVIGSVSVD FLQTETEQSI KKHLRNLSVI AVLVLLLGFI GAAVLAKSIR
210 220 230 240 250
KDTLGLEPHE IAALYRERNA MLFAIREGII ATNREGVVTM MNVSAAEMLK
260 270 280 290 300
LPEPVIHLPI DDVMPGAGLM SVLEKGEMLP NQEVSVNDQV FIINTKVMNQ
310 320 330 340 350
GGQAYGIVVS FREKTELKKL IDTLTEVRKY SEDLRAQTHE FSNKLYAILG
360 370 380 390 400
LLELGEYDEA IDLIKEEYAI QNEQHDLLFH NIHSQQVQAI LLGKISKASE
410 420 430 440 450
KKVKLVIDEN SSLAPLPAHI GLSHLITIIG NLIDNAFEAV AEQSVKEVLF
460 470 480 490 500
FITDMGHDIV IEVSDTGPGV PPEKIEAVFE RGYSSKGMRR GYGLANVKDS
510 520 530 540
VRELGGWIEL ANQKTGGAVF TVFIPKEKQR GNPFDSHRDC GG
Length:542
Mass (Da):59,891
Last modified:January 1, 1998 - v1
Checksum:iE0F3BBB9792F8C42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86417 Genomic DNA. Translation: BAA22311.1.
AL009126 Genomic DNA. Translation: CAB12587.1.
PIRiE69600.
RefSeqiNP_388639.1. NC_000964.3.
WP_003242967.1. NZ_JNCM01000032.1.

Genome annotation databases

EnsemblBacteriaiCAB12587; CAB12587; BSU07580.
GeneIDi939687.
KEGGibsu:BSU07580.
PATRICi18973166. VBIBacSub10457_0797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86417 Genomic DNA. Translation: BAA22311.1.
AL009126 Genomic DNA. Translation: CAB12587.1.
PIRiE69600.
RefSeqiNP_388639.1. NC_000964.3.
WP_003242967.1. NZ_JNCM01000032.1.

3D structure databases

ProteinModelPortaliO34427.
SMRiO34427. Positions 38-162, 204-315, 416-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34427. 23 interactions.
STRINGi224308.Bsubs1_010100004233.

Proteomic databases

PaxDbiO34427.

Protocols and materials databases

DNASUi939687.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12587; CAB12587; BSU07580.
GeneIDi939687.
KEGGibsu:BSU07580.
PATRICi18973166. VBIBacSub10457_0797.

Phylogenomic databases

eggNOGiCOG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiO34427.
KOiK11637.
OMAiARTISYM.
PhylomeDBiO34427.

Enzyme and pathway databases

BioCyciBSUB:BSU07580-MONOMER.
BRENDAi2.7.13.3. 658.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCITS_BACSU
AccessioniPrimary (citable) accession number: O34427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.