Reviewed, UniProtKB/Swiss-Prot O34427 (CITS_BACSU)

Names and origin

Protein names

Recommended name:
    Sensor protein citS
    EC=2.7.13.3

Gene names

Name:	citS
Synonyms:	yflR
Ordered Locus Names:	BSU07580

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system citT/citS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates citT in response to environmental citrate or Mg2+-citrate complex. Ref.4
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	Sensor protein citS		PRO_0000074735
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Potential
	Topological domain	35 – 175	141	Extracellular Potential
	Transmembrane	176 – 196	21	Potential
	Topological domain	197 – 542	346	Cytoplasmic Potential
	Domain	216 – 279	64	PAS
	Domain	336 – 528	193	Histidine kinase
Amino acid modifications
	Modified residue	339	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O34427-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E0F3BBB9792F8C42	FASTA	542	59,891
	10 20 30 40 50 60 MVKKRFHFSL QTKIMGLIAA LLVFVIGVLT ITLAVQHTQG ERRQAEQLAV QTARTISYMP 70 80 90 100 110 120 PVKELIERKD GHAAQTQEVI EQMKEQTGAF AIYVLNEKGD IRSASGKSGL KKLERSREIL 130 140 150 160 170 180 FGGSHVSETK ADGRRVIRGS APIIKEQKGY SQVIGSVSVD FLQTETEQSI KKHLRNLSVI 190 200 210 220 230 240 AVLVLLLGFI GAAVLAKSIR KDTLGLEPHE IAALYRERNA MLFAIREGII ATNREGVVTM 250 260 270 280 290 300 MNVSAAEMLK LPEPVIHLPI DDVMPGAGLM SVLEKGEMLP NQEVSVNDQV FIINTKVMNQ 310 320 330 340 350 360 GGQAYGIVVS FREKTELKKL IDTLTEVRKY SEDLRAQTHE FSNKLYAILG LLELGEYDEA 370 380 390 400 410 420 IDLIKEEYAI QNEQHDLLFH NIHSQQVQAI LLGKISKASE KKVKLVIDEN SSLAPLPAHI 430 440 450 460 470 480 GLSHLITIIG NLIDNAFEAV AEQSVKEVLF FITDMGHDIV IEVSDTGPGV PPEKIEAVFE 490 500 510 520 530 540 RGYSSKGMRR GYGLANVKDS VRELGGWIEL ANQKTGGAVF TVFIPKEKQR GNPFDSHRDC GG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein." Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J. Gene 194:191-199(1997) [PubMed: 9272861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / AC327.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis." Yamamoto H., Murata M., Sekiguchi J. Mol. Microbiol. 37:898-912(2000) [PubMed: 10972810] [Abstract] Cited for: CHARACTERIZATION. Strain: 168.
[4]	"Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems." Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T., Fujita Y. J. Bacteriol. 183:7365-7370(2001) [PubMed: 11717295] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86417 Genomic DNA. Translation: BAA22311.1. AL009126 Genomic DNA. Translation: CAB12587.1.
PIR	E69600.
RefSeq	NP_388639.1.
3D structure databases
SMR	O34427. Positions 43-168, 216-319, 421-527.
ModBase	Search...
Genome annotation databases
GeneID	939687.
GenomeReviews	Gene locus BSU07580 in contig AL009126_GR.
KEGG	bsu:BSU07580.
NMPDR	fig|224308.1.peg.758.
Organism-specific databases
SubtiList	BG12576. citS. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG537380.
OMA	ELGGWIE.
PhylomeDB	O34427.
Enzyme and pathway databases
BRENDA	2.7.13.3. 150.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR000014. PAS. IPR004358. Sig_transdc_His_kin-like_C. IPR016120. Sig_transdc_His_kin_SpoOB. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. PS50112. PAS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CITS_BACSU

Accession

Primary (citable) accession number: O34427

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	January 1, 1998
Last modified:	January 19, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)