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O34427 (CITS_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sensor protein CitS
EC=2.7.13.3
Gene names
Name:citS
Synonyms:yflR
Ordered Locus Names:BSU07580
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length542 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg2+-citrate complex. Ref.4

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Sequence similarities

Contains 1 histidine kinase domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
   Biological processTwo-component regulatory system
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpeptidyl-histidine phosphorylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

two-component sensor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 542542Sensor protein CitS
PRO_0000074735

Regions

Topological domain1 – 1313Cytoplasmic Potential
Transmembrane14 – 3421Helical; Potential
Topological domain35 – 175141Extracellular Potential
Transmembrane176 – 19621Helical; Potential
Topological domain197 – 542346Cytoplasmic Potential
Domain216 – 27964PAS
Domain336 – 528193Histidine kinase

Amino acid modifications

Modified residue3391Phosphohistidine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
O34427 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: E0F3BBB9792F8C42

FASTA54259,891
        10         20         30         40         50         60 
MVKKRFHFSL QTKIMGLIAA LLVFVIGVLT ITLAVQHTQG ERRQAEQLAV QTARTISYMP 

        70         80         90        100        110        120 
PVKELIERKD GHAAQTQEVI EQMKEQTGAF AIYVLNEKGD IRSASGKSGL KKLERSREIL 

       130        140        150        160        170        180 
FGGSHVSETK ADGRRVIRGS APIIKEQKGY SQVIGSVSVD FLQTETEQSI KKHLRNLSVI 

       190        200        210        220        230        240 
AVLVLLLGFI GAAVLAKSIR KDTLGLEPHE IAALYRERNA MLFAIREGII ATNREGVVTM 

       250        260        270        280        290        300 
MNVSAAEMLK LPEPVIHLPI DDVMPGAGLM SVLEKGEMLP NQEVSVNDQV FIINTKVMNQ 

       310        320        330        340        350        360 
GGQAYGIVVS FREKTELKKL IDTLTEVRKY SEDLRAQTHE FSNKLYAILG LLELGEYDEA 

       370        380        390        400        410        420 
IDLIKEEYAI QNEQHDLLFH NIHSQQVQAI LLGKISKASE KKVKLVIDEN SSLAPLPAHI 

       430        440        450        460        470        480 
GLSHLITIIG NLIDNAFEAV AEQSVKEVLF FITDMGHDIV IEVSDTGPGV PPEKIEAVFE 

       490        500        510        520        530        540 
RGYSSKGMRR GYGLANVKDS VRELGGWIEL ANQKTGGAVF TVFIPKEKQR GNPFDSHRDC 


GG

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein."
Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J.
Gene 194:191-199(1997) [PubMed: 9272861] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / AC327.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis."
Yamamoto H., Murata M., Sekiguchi J.
Mol. Microbiol. 37:898-912(2000) [PubMed: 10972810] [Abstract]
Cited for: CHARACTERIZATION.
Strain: 168.
[4]"Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems."
Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T., Fujita Y.
J. Bacteriol. 183:7365-7370(2001) [PubMed: 11717295] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D86417 Genomic DNA. Translation: BAA22311.1.
AL009126 Genomic DNA. Translation: CAB12587.1.
PIRE69600.
RefSeqNP_388639.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34427.
SMRO34427. Positions 38-162, 204-315, 416-527.
ModBaseSearch...

Protein-protein interaction databases

IntActO34427. 23 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000001891; EBBACP00000001891; EBBACG00000001888.
GeneID939687.
GenomeReviewsGene locus BSU07580 in contig AL009126_GR.
KEGGbsu:BSU07580.
NMPDRfig|224308.1.peg.758.
PATRIC18973166. VBIBacSub10457_0797.

Organism-specific databases

GenoListBSU07580. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000000017.
HOGENOMHBG537380.
OMAYRERNAM.
PhylomeDBO34427.
ProtClustDBCLSK886855.

Enzyme and pathway databases

BioCycBSUB:BSU07580-MONOMER.
BRENDA2.7.13.3. 700.

Family and domain databases

InterProIPR003594. ATPase-like_ATP-bd.
IPR000014. PAS.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
IPR005467. Sig_transdc_His_kinase_core.
IPR016121. Spore_sensor_kin_SpoOB_hlx.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:1.10.287.130. Sensor_kinase_SpoOB-type_hlx. 1 hit.
KOK11637.
PfamPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSPR00344. BCTRLSENSOR.
SMARTSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF55890. Sensor_kinase_SpoOB-type. 1 hit.
PROSITEPS50109. HIS_KIN. 1 hit.
PS50112. PAS. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCITS_BACSU
AccessionPrimary (citable) accession number: O34427
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 1, 1998
Last modified: January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents