ID   GLTB_BACSU              Reviewed;         493 AA.
AC   O34399;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=Glutamate synthase [NADPH] small chain;
DE            EC=1.4.1.13;
DE   AltName: Full=NADPH-GOGAT;
GN   Name=gltB; OrderedLocusNames=BSU18440;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   SEQUENCE REVISION TO 354.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G.,
RA   Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus
RT   subtilis 168 reference genome a decade later.";
RL   Microbiology 0:0-0(2009).
RN   [3]
RP   INDUCTION.
RX   MEDLINE=20485542; PubMed=11029411;
RX   DOI=10.1128/JB.182.21.5939-5947.2000;
RA   Belitsky B.R., Wray L.V. Jr., Fisher S.H., Bohannon D.E.,
RA   Sonenshein A.L.;
RT   "Role of TnrA in nitrogen source-dependent repression of Bacillus
RT   subtilis glutamate synthase gene expression.";
RL   J. Bacteriol. 182:5939-5947(2000).
CC   -!- CATALYTIC ACTIVITY: 2 L-glutamate + NADP(+) = L-glutamine + 2-
CC       oxoglutarate + NADPH.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-glutamate biosynthesis via GLT
CC       pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NADPH
CC       route): step 1/1.
CC   -!- PATHWAY: Energy metabolism; nitrogen metabolism.
CC   -!- SUBUNIT: Aggregate of 4 catalytic active heterodimers, consisting
CC       of a large and a small subunit (By similarity).
CC   -!- INDUCTION: The gltAB operon is positively regulated by gltC and
CC       repressed by tnrA.
CC   -!- SIMILARITY: Belongs to the glutamate synthase family.
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DR   EMBL; AL009126; CAB13727.2; -; Genomic_DNA.
DR   PIR; H69634; H69634.
DR   RefSeq; NP_389726.1; -.
DR   GeneID; 940053; -.
DR   GenomeReviews; AL009126_GR; BSU18440.
DR   KEGG; bsu:BSU18440; -.
DR   NMPDR; fig|224308.1.peg.1848; -.
DR   SubtiList; BG12594; gltB.
DR   HOGENOM; O34399; -.
DR   OMA; O34399; PIPGTEF.
DR   BioCyc; BSUB224308:BSU1844-MON; -.
DR   BRENDA; 1.4.1.13; 150.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004355; F:glutamate synthase (NADPH) activity; IEA:EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0006537; P:glutamate biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000759; Adrndx_reductase.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR012285; Fum_reductase_C.
DR   InterPro; IPR006005; Glut_synth_sub1.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Gene3D; G3DSA:1.10.1060.10; Fum_reductase_C; 1.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PRINTS; PR00419; ADXRDTASE.
DR   TIGRFAMs; TIGR01317; GOGAT_sm_gam; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Complete proteome; Glutamate biosynthesis;
KW   NADP; Oxidoreductase.
FT   CHAIN         1    493       Glutamate synthase [NADPH] small chain.
FT                                /FTId=PRO_0000170798.
FT   NP_BIND     299    313       NADP (Potential).
SQ   SEQUENCE   493 AA;  54862 MW;  EBEA7171A4AA0270 CRC64;
     MGKPTGFMEI KREKPAERDP LTRLKDWKEY SAPFSEEASK RQGARCMDCG TPFCQIGADI
     NGFTSGCPIY NLIPEWNDLV YRGRWKEALE RLLKTNNFPE FTGRVCPAPC EGSCTLAISD
     PAVSIKNIER TIIDKGFENG WIQPRIPKKR TGKKVAIVGS GPAGLASADQ LNQAGHSVTV
     FERADRAGGL LTYGIPNMKL EKGIVERRIK LLTQEGIDFV TNTEIGVDIT ADELKEQFDA
     VILCTGAQKQ RDLLIEGRDS KGVHYAMDYL TLATKSYLDS NFKDKQFIDA KGKDVIVIGG
     GDTGADCVAT ALRQKAKSVH QFGKHPKLPP ARTNDNMWPE QPHVFTLEYA YEEAEAKFGR
     DPREYSIQTT KMVADKNGKL KELHTIQMEK VKNEHGKYEF RELPGTEKVW PAQLVFIAIG
     FEGTEQPLLK QFGVNSVNNK ISAAYGDYQT NIDGVFAAGD ARRGQSLIVW AINEGREVAR
     EVDRYLMGSS VLP
//