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Protein

Glutamate synthase [NADPH] small chain

Gene

gltB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH.

Pathwayi: L-glutamate biosynthesis via GLT pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamate synthase [NADPH] small chain (gltB), Glutamate synthase [NADPH] large chain (gltA)
This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

Pathwayi: nitrogen metabolism

This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi299 – 31315NADPSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Glutamate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBSUB:BSU18440-MONOMER.
RETL1328306-WGS:GSTH-3633-MONOMER.
SABIO-RKO34399.
UniPathwayiUPA00045.
UPA00634; UER00689.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate synthase [NADPH] small chain (EC:1.4.1.13)
Alternative name(s):
NADPH-GOGAT
Gene namesi
Name:gltB
Ordered Locus Names:BSU18440
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Glutamate synthase [NADPH] small chainPRO_0000170798Add
BLAST

Proteomic databases

PaxDbiO34399.

Expressioni

Inductioni

The gltAB operon is positively regulated by GltC and negatively regulated by TnrA under nitrogen-limited conditions.

Interactioni

Subunit structurei

Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.By similarity

Protein-protein interaction databases

IntActiO34399. 1 interaction.
MINTiMINT-8365908.
STRINGi224308.Bsubs1_010100010161.

Structurei

3D structure databases

ProteinModelPortaliO34399.
SMRiO34399. Positions 4-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ8. Bacteria.
COG0493. LUCA.
HOGENOMiHOG000031443.
InParanoidiO34399.
KOiK00266.
OMAiSCTLNID.
PhylomeDBiO34399.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR006005. Glut_synth_ssu1.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR01317. GOGAT_sm_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

O34399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKPTGFMEI KREKPAERDP LTRLKDWKEY SAPFSEEASK RQGARCMDCG
60 70 80 90 100
TPFCQIGADI NGFTSGCPIY NLIPEWNDLV YRGRWKEALE RLLKTNNFPE
110 120 130 140 150
FTGRVCPAPC EGSCTLAISD PAVSIKNIER TIIDKGFENG WIQPRIPKKR
160 170 180 190 200
TGKKVAIVGS GPAGLASADQ LNQAGHSVTV FERADRAGGL LTYGIPNMKL
210 220 230 240 250
EKGIVERRIK LLTQEGIDFV TNTEIGVDIT ADELKEQFDA VILCTGAQKQ
260 270 280 290 300
RDLLIEGRDS KGVHYAMDYL TLATKSYLDS NFKDKQFIDA KGKDVIVIGG
310 320 330 340 350
GDTGADCVAT ALRQKAKSVH QFGKHPKLPP ARTNDNMWPE QPHVFTLEYA
360 370 380 390 400
YEEAEAKFGR DPREYSIQTT KMVADKNGKL KELHTIQMEK VKNEHGKYEF
410 420 430 440 450
RELPGTEKVW PAQLVFIAIG FEGTEQPLLK QFGVNSVNNK ISAAYGDYQT
460 470 480 490
NIDGVFAAGD ARRGQSLIVW AINEGREVAR EVDRYLMGSS VLP
Length:493
Mass (Da):54,862
Last modified:June 16, 2009 - v2
Checksum:iEBEA7171A4AA0270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13727.2.
PIRiH69634.
RefSeqiNP_389726.2. NC_000964.3.
WP_003231462.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13727; CAB13727; BSU18440.
GeneIDi940053.
KEGGibsu:BSU18440.
PATRICi18975527. VBIBacSub10457_1954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13727.2.
PIRiH69634.
RefSeqiNP_389726.2. NC_000964.3.
WP_003231462.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO34399.
SMRiO34399. Positions 4-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34399. 1 interaction.
MINTiMINT-8365908.
STRINGi224308.Bsubs1_010100010161.

Proteomic databases

PaxDbiO34399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13727; CAB13727; BSU18440.
GeneIDi940053.
KEGGibsu:BSU18440.
PATRICi18975527. VBIBacSub10457_1954.

Phylogenomic databases

eggNOGiENOG4105BZ8. Bacteria.
COG0493. LUCA.
HOGENOMiHOG000031443.
InParanoidiO34399.
KOiK00266.
OMAiSCTLNID.
PhylomeDBiO34399.

Enzyme and pathway databases

UniPathwayiUPA00045.
UPA00634; UER00689.
BioCyciBSUB:BSU18440-MONOMER.
RETL1328306-WGS:GSTH-3633-MONOMER.
SABIO-RKO34399.

Miscellaneous databases

PROiO34399.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR006005. Glut_synth_ssu1.
IPR009051. Helical_ferredxn.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR01317. GOGAT_sm_gam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLTB_BACSU
AccessioniPrimary (citable) accession number: O34399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: June 16, 2009
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.