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Protein

Glutamate synthase [NADPH] small chain

Gene

gltB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH.

Pathwayi: L-glutamate biosynthesis via GLT pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamate synthase [NADPH] small chain (gltB), Glutamate synthase [NADPH] large chain (gltA)
This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

Pathwayi: nitrogen metabolism

This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi299 – 313NADPSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Glutamate biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciBSUB:BSU18440-MONOMER
SABIO-RKiO34399
UniPathwayiUPA00045
UPA00634; UER00689

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate synthase [NADPH] small chain (EC:1.4.1.13)
Alternative name(s):
NADPH-GOGAT
Gene namesi
Name:gltB
Ordered Locus Names:BSU18440
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001707981 – 493Glutamate synthase [NADPH] small chainAdd BLAST493

Proteomic databases

PaxDbiO34399

Expressioni

Inductioni

The gltAB operon is positively regulated by GltC and negatively regulated by TnrA under nitrogen-limited conditions.

Interactioni

Subunit structurei

Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.By similarity

Protein-protein interaction databases

IntActiO34399, 1 interactor
MINTiO34399
STRINGi224308.Bsubs1_010100010161

Structurei

3D structure databases

ProteinModelPortaliO34399
SMRiO34399
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ8 Bacteria
COG0493 LUCA
HOGENOMiHOG000031443
InParanoidiO34399
KOiK00266
OMAiTQFELMP
PhylomeDBiO34399

Family and domain databases

Gene3Di1.10.1060.10, 1 hit
3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR028261 DPD_II
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR006005 Glut_synth_ssu1
IPR009051 Helical_ferredxn
PfamiView protein in Pfam
PF14691 Fer4_20, 1 hit
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF46548 SSF46548, 1 hit
TIGRFAMsiTIGR01317 GOGAT_sm_gam, 1 hit

Sequencei

Sequence statusi: Complete.

O34399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKPTGFMEI KREKPAERDP LTRLKDWKEY SAPFSEEASK RQGARCMDCG
60 70 80 90 100
TPFCQIGADI NGFTSGCPIY NLIPEWNDLV YRGRWKEALE RLLKTNNFPE
110 120 130 140 150
FTGRVCPAPC EGSCTLAISD PAVSIKNIER TIIDKGFENG WIQPRIPKKR
160 170 180 190 200
TGKKVAIVGS GPAGLASADQ LNQAGHSVTV FERADRAGGL LTYGIPNMKL
210 220 230 240 250
EKGIVERRIK LLTQEGIDFV TNTEIGVDIT ADELKEQFDA VILCTGAQKQ
260 270 280 290 300
RDLLIEGRDS KGVHYAMDYL TLATKSYLDS NFKDKQFIDA KGKDVIVIGG
310 320 330 340 350
GDTGADCVAT ALRQKAKSVH QFGKHPKLPP ARTNDNMWPE QPHVFTLEYA
360 370 380 390 400
YEEAEAKFGR DPREYSIQTT KMVADKNGKL KELHTIQMEK VKNEHGKYEF
410 420 430 440 450
RELPGTEKVW PAQLVFIAIG FEGTEQPLLK QFGVNSVNNK ISAAYGDYQT
460 470 480 490
NIDGVFAAGD ARRGQSLIVW AINEGREVAR EVDRYLMGSS VLP
Length:493
Mass (Da):54,862
Last modified:June 16, 2009 - v2
Checksum:iEBEA7171A4AA0270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13727.2
PIRiH69634
RefSeqiNP_389726.2, NC_000964.3
WP_003231462.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB13727; CAB13727; BSU18440
GeneIDi940053
KEGGibsu:BSU18440
PATRICifig|224308.179.peg.2011

Similar proteinsi

Entry informationi

Entry nameiGLTB_BACSU
AccessioniPrimary (citable) accession number: O34399
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health