Reviewed, UniProtKB/Swiss-Prot O34389 (MAO3_BACSU)

Names and origin

Protein names

Recommended name:
    Probable NAD-dependent malic enzyme 3
      Short name=NAD-ME 3
    EC=1.1.1.38

Gene names

Name:	malS
Ordered Locus Names:	BSU29880

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Probable NAD-dependent malic enzyme 3		PRO_0000160210
Sites
	Active site	105	1	Proton donor By similarity
	Active site	178	1	Proton acceptor By similarity
	Metal binding	249	1	Divalent metal cation By similarity
	Metal binding	250	1	Divalent metal cation By similarity
	Metal binding	273	1	Divalent metal cation By similarity
	Binding site	273	1	NAD By similarity
	Binding site	423	1	NAD By similarity
	Site	273	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O34389-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 226C658154548270	FASTA	566	62,149
	10 20 30 40 50 60 MKQFRVTNEG DIQTTLRGLE VLSVPFLNKG VAFTEEERKE LGLKGFLPPK VLTIDDQAKR 70 80 90 100 110 120 AYEQYSAQPD DLSKNVYLTA LHDRNETLFY RLLNDHLGEM LPIVYTPTVG TAIQRYSHEY 130 140 150 160 170 180 RKPRGLYLSI DDPDGMKEAF KQYKDQSDTI DLIVATDAEG ILGIGDWGVG GIAISIGKLA 190 200 210 220 230 240 VYTAAAGIDP SRVLAVVLDA GTNQESLLND PLYVGNQHSR VRGERYDQFI DDYVALARET 250 260 270 280 290 300 FPNALLHWED FGAKNARSIL KRYKDKVCTF NDDIQGTGAV SLAAVLSCAK ASKVPLRDHR 310 320 330 340 350 360 VVIFGAGTAG IGIAEQLREA LVREGLSEEE SYKRFWCIDR NGLLTDDMDQ LLDFQKPYAR 370 380 390 400 410 420 SADEVKDYQR NGDGGGIDLL EVVRQAKPTI LIGTSTVSGA FTEEIVKEMA SHVKRPAILP 430 440 450 460 470 480 MSNPTTLSEA KPEDLIEWTE GRALITTGSP FPPVEYNGVT YHIGQANNAL VFPGLGLGTI 490 500 510 520 530 540 VTKSKLITDG MFEACARAIA GMVNVGVPGA PMLPKVEDLR TVSATVAVEV AKTAMKEGVA 550 560 TEEPEDIIQA VQDAMWYPVY KPIRAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008220 Genomic DNA. Translation: AAC00287.1. AL009126 Genomic DNA. Translation: CAB14966.1.
PIR	D69655.
RefSeq	NP_390866.1.
3D structure databases
SMR	O34389. Positions 17-564.
ModBase	Search...
Genome annotation databases
GeneID	938071.
GenomeReviews	Gene locus BSU29880 in contig AL009126_GR.
KEGG	bsu:BSU29880.
NMPDR	fig|224308.1.peg.2991.
Organism-specific databases
SubtiList	BG12614. malS. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289821.
OMA	IQDNNET.
PhylomeDB	O34389.
Enzyme and pathway databases
BRENDA	1.1.1.38. 150.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO3_BACSU

Accession

Primary (citable) accession number: O34389

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	January 1, 1998
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)