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Protein

Probable NAD-dependent malic enzyme 3

Gene

malS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105Proton donorBy similarity1
Active sitei178Proton acceptorBy similarity1
Metal bindingi249Divalent metal cationBy similarity1
Metal bindingi250Divalent metal cationBy similarity1
Metal bindingi273Divalent metal cationBy similarity1
Binding sitei273NADBy similarity1
Sitei273Important for activityBy similarity1
Binding sitei423NADBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU29880-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NAD-dependent malic enzyme 3 (EC:1.1.1.38)
Short name:
NAD-ME 3
Gene namesi
Name:malS
Ordered Locus Names:BSU29880
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602101 – 566Probable NAD-dependent malic enzyme 3Add BLAST566

Proteomic databases

PaxDbiO34389.
PRIDEiO34389.

Interactioni

Protein-protein interaction databases

IntActiO34389. 1 interactor.
STRINGi224308.Bsubs1_010100016296.

Structurei

3D structure databases

ProteinModelPortaliO34389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C6K. Bacteria.
COG0281. LUCA.
HOGENOMiHOG000042487.
InParanoidiO34389.
KOiK00027.
OMAiLNDPMYM.
PhylomeDBiO34389.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQFRVTNEG DIQTTLRGLE VLSVPFLNKG VAFTEEERKE LGLKGFLPPK
60 70 80 90 100
VLTIDDQAKR AYEQYSAQPD DLSKNVYLTA LHDRNETLFY RLLNDHLGEM
110 120 130 140 150
LPIVYTPTVG TAIQRYSHEY RKPRGLYLSI DDPDGMKEAF KQYKDQSDTI
160 170 180 190 200
DLIVATDAEG ILGIGDWGVG GIAISIGKLA VYTAAAGIDP SRVLAVVLDA
210 220 230 240 250
GTNQESLLND PLYVGNQHSR VRGERYDQFI DDYVALARET FPNALLHWED
260 270 280 290 300
FGAKNARSIL KRYKDKVCTF NDDIQGTGAV SLAAVLSCAK ASKVPLRDHR
310 320 330 340 350
VVIFGAGTAG IGIAEQLREA LVREGLSEEE SYKRFWCIDR NGLLTDDMDQ
360 370 380 390 400
LLDFQKPYAR SADEVKDYQR NGDGGGIDLL EVVRQAKPTI LIGTSTVSGA
410 420 430 440 450
FTEEIVKEMA SHVKRPAILP MSNPTTLSEA KPEDLIEWTE GRALITTGSP
460 470 480 490 500
FPPVEYNGVT YHIGQANNAL VFPGLGLGTI VTKSKLITDG MFEACARAIA
510 520 530 540 550
GMVNVGVPGA PMLPKVEDLR TVSATVAVEV AKTAMKEGVA TEEPEDIIQA
560
VQDAMWYPVY KPIRAI
Length:566
Mass (Da):62,149
Last modified:January 1, 1998 - v1
Checksum:i226C658154548270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00287.1.
AL009126 Genomic DNA. Translation: CAB14966.1.
PIRiD69655.
RefSeqiNP_390866.1. NC_000964.3.
WP_003229258.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14966; CAB14966; BSU29880.
GeneIDi938071.
KEGGibsu:BSU29880.
PATRICi18977882. VBIBacSub10457_3129.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA. Translation: AAC00287.1.
AL009126 Genomic DNA. Translation: CAB14966.1.
PIRiD69655.
RefSeqiNP_390866.1. NC_000964.3.
WP_003229258.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO34389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO34389. 1 interactor.
STRINGi224308.Bsubs1_010100016296.

Proteomic databases

PaxDbiO34389.
PRIDEiO34389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14966; CAB14966; BSU29880.
GeneIDi938071.
KEGGibsu:BSU29880.
PATRICi18977882. VBIBacSub10457_3129.

Phylogenomic databases

eggNOGiENOG4105C6K. Bacteria.
COG0281. LUCA.
HOGENOMiHOG000042487.
InParanoidiO34389.
KOiK00027.
OMAiLNDPMYM.
PhylomeDBiO34389.

Enzyme and pathway databases

BioCyciBSUB:BSU29880-MONOMER.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAO3_BACSU
AccessioniPrimary (citable) accession number: O34389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.