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O34364

- O16G2_BACSU

UniProt

O34364 - O16G2_BACSU

Protein

Probable oligo-1,6-glucosidase 2

Gene

ycdG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei199 – 1991NucleophileBy similarity
    Active sitei255 – 2551Proton donorBy similarity
    Sitei330 – 3301Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. oligo-1,6-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU02840-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable oligo-1,6-glucosidase 2 (EC:3.2.1.10)
    Alternative name(s):
    Oligosaccharide alpha-1,6-glucosidase 2
    Sucrase-isomaltase 2
    Short name:
    Isomaltase 2
    Gene namesi
    Name:ycdG
    Ordered Locus Names:BSU02840
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU02840.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 561561Probable oligo-1,6-glucosidase 2PRO_0000360818Add
    BLAST

    Proteomic databases

    PaxDbiO34364.

    Expressioni

    Inductioni

    By ethanol, heat and salt via sigma B-dependent promoter.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU02840.

    Structurei

    3D structure databases

    ProteinModelPortaliO34364.
    SMRiO34364. Positions 1-558.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    HOGENOMiHOG000220641.
    KOiK01182.
    OMAiEERNDQT.
    OrthoDBiEOG6RVFV2.
    PhylomeDBiO34364.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O34364-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTDWWKDAV VYQIYPRSFQ DSNGDGIGDL RGIISRLDYI KELGADVIWI    50
    CPIYPSPNVD YGYDVTNHKA IMDSYGTMDD FHELLDQVHQ RGLKLVMDFV 100
    LNHTSVEHPW FKEAELDKNS KYRSYYYWRP GTKNGPPTDW LSNYGCPVWQ 150
    YEEHTGEYYL HMNAVKQADL NWENPEVRQA VYDMMKFWLD KGVDGLRIDQ 200
    LHLISKKEYL PSYEDYINQQ AEPKPFQPNG ERIHDYLKEI TDEVFSHYDV 250
    MSVGEVGSVT PEEGLKYTGT DKHELNMIFH FQHMELDQQP GKEHWDLKPL 300
    ELSDLKSVLT KWQKKLEHQG WNTLFWCNHD QPRIVSRFGD DGEYRKASAK 350
    MLAAVIYFMK GTPYIYQGEE IGMTNAPFTR IEDYKDIQTI NMYHKRVFEK 400
    GYDPNDVMRS ILAKSRDHAR TPMQWNSGKN AGFTDGTPWL KVNPNFTAIN 450
    VEEAQGDPDS VLNYYKKLIS LRKQYADLMK GSFDLLLPDD PQLFVYMREN 500
    SKQQLLSVNN FSKEQAVFQW PKNCGKAQAS LLLSNYNNDD LDDEMVFRPY 550
    ESRVYLLDKT N 561
    Length:561
    Mass (Da):65,816
    Last modified:January 1, 1998 - v1
    Checksum:i1E879220CAE04BBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000617 Genomic DNA. Translation: BAA22245.1.
    AL009126 Genomic DNA. Translation: CAB12078.1.
    PIRiH69755.
    RefSeqiNP_388166.1. NC_000964.3.
    WP_003234736.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12078; CAB12078; BSU02840.
    GeneIDi938375.
    KEGGibsu:BSU02840.
    PATRICi18972127. VBIBacSub10457_0292.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB000617 Genomic DNA. Translation: BAA22245.1 .
    AL009126 Genomic DNA. Translation: CAB12078.1 .
    PIRi H69755.
    RefSeqi NP_388166.1. NC_000964.3.
    WP_003234736.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali O34364.
    SMRi O34364. Positions 1-558.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU02840.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi O34364.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12078 ; CAB12078 ; BSU02840 .
    GeneIDi 938375.
    KEGGi bsu:BSU02840.
    PATRICi 18972127. VBIBacSub10457_0292.

    Organism-specific databases

    GenoListi BSU02840.

    Phylogenomic databases

    eggNOGi COG0366.
    HOGENOMi HOG000220641.
    KOi K01182.
    OMAi EERNDQT.
    OrthoDBi EOG6RVFV2.
    PhylomeDBi O34364.

    Enzyme and pathway databases

    BioCyci BSUB:BSU02840-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutation."
      Kumano M., Tamakoshi A., Yamane K.
      Microbiology 143:2775-2782(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Global analysis of the general stress response of Bacillus subtilis."
      Petersohn A., Brigulla M., Haas S., Hoheisel J.D., Voelker U., Hecker M.
      J. Bacteriol. 183:5617-5631(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiO16G2_BACSU
    AccessioniPrimary (citable) accession number: O34364
    Secondary accession number(s): Q797R7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3