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Protein

Serine protease Do-like HtrA

Gene

htrA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Degrades abnormal exported proteins and responsible for the propeptide processing of a natural pro-protein and for the maturation of a native protein. It also plays a prominent role in stress (heat shock, ethanol, puromycin and NaCl) resistance during active exponential growth (Probable).1 Publication

Miscellaneous

Inactivation results in compensating overexpression of YtvA, especially during stress conditions.

Catalytic activityi

Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei179Charge relay systemSequence analysis1
Active sitei209Charge relay systemSequence analysis1
Active sitei290Charge relay systemSequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processStress response

Enzyme and pathway databases

BioCyciBSUB:BSU12900-MONOMER

Protein family/group databases

MEROPSiS01.B81

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease Do-like HtrA (EC:3.4.21.107)
Alternative name(s):
HtrA-like serine protease
Gene namesi
Name:htrA
Synonyms:ykdA
Ordered Locus Names:BSU12900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 44CytoplasmicSequence analysisAdd BLAST44
Transmembranei45 – 67HelicalSequence analysisAdd BLAST23
Topological domaini68 – 449ExtracellularSequence analysisAdd BLAST382

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

In contrast to other bacteria, in which inactivation of serine protease leads to thermosensitivity, inactivation of HtrA leads to an increased thermotolerance and an increased tolerance to hydrogen peroxide. Inactivation of both HtrA and HtrB leads to growth defects and to thermosensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938591 – 449Serine protease Do-like HtrAAdd BLAST449

Proteomic databases

PaxDbiO34358
PRIDEiO34358

Expressioni

Inductioni

Transcription is CssS dependent. Induced by heat shock during exponential growth and by heterologous amylases at the transition phase of the growth cycle. Negatively regulates its own expression during exponential growth and during heat shock.3 Publications

Interactioni

Protein-protein interaction databases

IntActiO34358, 3 interactors
STRINGi224308.Bsubs1_010100007156

Structurei

3D structure databases

ProteinModelPortaliO34358
SMRiO34358
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini348 – 437PDZPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni288 – 290Substrate bindingBy similarity3
Regioni344 – 348Substrate bindingBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi103 – 108Poly-Ser6
Compositional biasi146 – 152Poly-Ser7

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C0H Bacteria
COG0265 LUCA
HOGENOMiHOG000223641
InParanoidiO34358
KOiK04771
OMAiCFCLHSS
PhylomeDBiO34358

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR009003 Peptidase_S1_PA
IPR001940 Peptidase_S1C
PfamiView protein in Pfam
PF13180 PDZ_2, 1 hit
PRINTSiPR00834 PROTEASES2C
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit

Sequencei

Sequence statusi: Complete.

O34358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNYRDENRT KGNENEVFLT KENDQSASYS ARNVIHDQEK KKRGFGWFRP
60 70 80 90 100
LLGGVIGGSL ALGIYTFTPL GDHDSQDTAK QSSSQQQTQS VTATSTSSES
110 120 130 140 150
KKSSSSSSAF KSEDSSKISD MVEDLSPAIV GITNLQAQSN SSLFGSSSSD
160 170 180 190 200
SSEDTESGSG SGVIFKKENG KAYIITNNHV VEGASSLKVS LYDGTEVTAK
210 220 230 240 250
LVGSDSLTDL AVLQISDDHV TKVANFGDSS DLRTGETVIA IGDPLGKDLS
260 270 280 290 300
RTVTQGIVSG VDRTVSMSTS AGETSINVIQ TDAAINPGNS GGPLLNTDGK
310 320 330 340 350
IVGINSMKIS EDDVEGIGFA IPSNDVKPIA EELLSKGQIE RPYIGVSMLD
360 370 380 390 400
LEQVPQNYQE GTLGLFGSQL NKGVYIREVA SGSPAEKAGL KAEDIIIGLK
410 420 430 440
GKEIDTGSEL RNILYKDAKI GDTVEVKILR NGKEMTKKIK LDQKEEKTS
Length:449
Mass (Da):47,715
Last modified:June 16, 2009 - v2
Checksum:i8E4A28CDE18C4612
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72D → N in CAA05570 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002571 Genomic DNA Translation: CAA05570.1
AL009126 Genomic DNA Translation: CAB13147.2
PIRiA69643
RefSeqiNP_389173.2, NC_000964.3
WP_009967069.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13147; CAB13147; BSU12900
GeneIDi939849
KEGGibsu:BSU12900
PATRICifig|224308.179.peg.1402

Similar proteinsi

Entry informationi

Entry nameiHTRA_BACSU
AccessioniPrimary (citable) accession number: O34358
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health