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O34303 (YDJA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsuMI component YdjA

Short name=R.BsuM
Short name=R.BsuMI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsuMI component YdjA
Type II restriction enzyme BsuMI component YdjA
Gene names
Name:ydjA
Ordered Locus Names:BSU06110
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTCGAG; the cleavage site is unknown.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

BsuMI restriction activity requires YdiR, YdiS and YdjA.

Developmental stage

Not expressed during sporulation. Ref.4

Induction

Constitutively expressed during exponential growth. Encoded in an operon with ydiR and ydiS. Ref.4

Disruption phenotype

Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences. Ref.4

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Type-2 restriction enzyme BsuMI component YdjA
PRO_0000379883

Sequences

Sequence LengthMass (Da)Tools
O34303 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: DE93019E964B0E65

FASTA46554,336
        10         20         30         40         50         60 
MDKSSKFFFE DQKYNKERIV RVLGGNLALL KSKGILYEDS SGDLIFNYVG VISNGRNVIF 

        70         80         90        100        110        120 
ILPKYCNRHL DEHSKRTLFN KLLKIFKKYS GLNKSRESDY FVSELDSDEV SDFMIADYLL 

       130        140        150        160        170        180 
NDFSLNGYYQ KKFTEYEIDG EGIIDWSKTV NEITPVFSKG VPYYFSTYNE VVQKDEYHLI 

       190        200        210        220        230        240 
VKIHKWALSK YFNDFGVILG FTGLEFDKSC DGMKILDYAD FFGSVINKEI VNTYVDRDVK 

       250        260        270        280        290        300 
LLKALKTAID REENQFSKRP TLSLYGTKYF HRVWEEVCKT VFSHVNEYVK KISRPNWINF 

       310        320        330        340        350        360 
TDIEVNKEKK TLEPDIIKAF EYRSKEYFLI LDAKYYNINF DGKKLEGNPG VEDITKQLLY 

       370        380        390        400        410        420 
DKALEKLSRG KTKHNAFLFP SSNSTNTFKV FGSVDFDFLD IAAVTLVYIS AEQVYNLYLE 

       430        440        450        460 
NKTFSTDDLF KFVSEINKSK KRHSVITSTL YGNMFLFTKR LSDKN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes."
Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.
DNA Res. 4:335-339(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation."
Bron S., Janniere L., Ehrlich S.D.
Mol. Gen. Genet. 211:186-189(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA TARGET SEQUENCE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[4]"Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg."
Ohshima H., Matsuoka S., Asai K., Sadaie Y.
J. Bacteriol. 184:381-389(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, INDUCTION, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007637 Genomic DNA. Translation: BAA22755.1.
AL009126 Genomic DNA. Translation: CAB12430.1.
PIRE69788.
RefSeqNP_388492.1. NC_000964.3.

3D structure databases

ProteinModelPortalO34303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU06110.

Protein family/group databases

REBASE619. BsuMI.

Proteomic databases

PaxDbO34303.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12430; CAB12430; BSU06110.
GeneID939884.
KEGGbsu:BSU06110.
PATRIC18972858. VBIBacSub10457_0643.

Organism-specific databases

GenoListBSU06110.

Phylogenomic databases

eggNOGNOG15592.
HOGENOMHOG000080449.
OMAKERWISI.
OrthoDBEOG6PW23V.

Enzyme and pathway databases

BioCycBSUB:BSU06110-MONOMER.

Family and domain databases

InterProIPR018579. Restrct_endonuc_II_LlaJI.
[Graphical view]
PfamPF09563. RE_LlaJI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYDJA_BACSU
AccessionPrimary (citable) accession number: O34303
Secondary accession number(s): Q797C8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList