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Reviewed, UniProtKB/Swiss-Prot O34215 (GADH2_PECCY)

Last modified February 9, 2010. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Gluconate 2-dehydrogenase cytochrome c subunit
      Short name=GADH cytochrome c subunit
      Short name=GA 2-DH cytochrome c subunit
    EC=1.1.99.3
OrganismPectobacterium cypripedii (Erwinia cypripedii)
Taxonomic identifier55209 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate.

Catalytic activity

D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor.

Cofactor

FAD.

Subunit structure

Heterotrimer.

Subcellular location

Cell membrane; Peripheral membrane protein; Periplasmic side Potential.

Post-translational modification

Binds 3 heme groups per subunit Potential.

Sequence similarities

Contains 3 cytochrome c domains.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.0.

Temperature dependence:

Optimum temperature is 30 degrees Celsius.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.1
Chain20 – 441422Gluconate 2-dehydrogenase cytochrome c subunit
PRO_0000045855

Regions

Domain26 – 129104Cytochrome c 1
Domain173 – 289117Cytochrome c 2
Domain312 – 40392Cytochrome c 3

Sites

Metal binding441Iron (heme 1 axial ligand) By similarity
Metal binding1921Iron (heme 2 axial ligand) By similarity
Metal binding3291Iron (heme 3 axial ligand) By similarity
Binding site401Heme 1 (covalent) By similarity
Binding site431Heme 1 (covalent) By similarity
Binding site1881Heme 2 (covalent) By similarity
Binding site1911Heme 2 (covalent) By similarity
Binding site3251Heme 3 (covalent) By similarity
Binding site3281Heme 3 (covalent) By similarity

Sequences

Sequence LengthMass (Da)Tools
O34215-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 0AF512CFA99DA123

FASTA44147,097
        10         20         30         40         50         60 
MMKSILALVL GTLSFAALAD DQANDALVKR GEYLARAGDC VACHSVKGGQ PFAGGLPMAT 

        70         80         90        100        110        120 
PIGTIYSTNI TPDKTTGIGD YSYDDFQKAV RHGVAKNGDT LYPAMPYPSY AVVSDEDMKA 

       130        140        150        160        170        180 
LYAYFMHGVA PVAQANKDSD IPWPLSMRWP LAIWRGVFAP DVKAFQPAAQ EDPVLARGRY 

       190        200        210        220        230        240 
LVEGLGHCGA CHTPRSITMQ EKALSNDGAH DYLSGSSAPI DGWTASNLRG DNRDGLGRWS 

       250        260        270        280        290        300 
EDDLRQFLRY GRNDHTAAFG GMTDVVEHSL QHLSDDDITA IARYLKSLGA KDASQTVFTQ 

       310        320        330        340        350        360 
DDQVAKALWK GDDSQTGASV YVDSCAACHK TDGSRLSALL PGAAWQPGGA GEPDPTSLIH 

       370        380        390        400        410        420 
IVLTGGTLPG VQGAPTAITM PAFGWRLNDQ QVADVVNFIR GSWGNGAKAT VTAKDVASLR 

       430        440 
KDETVQAHQG NADIKVLEQQ Q 

« Hide

References

[1]"Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli."
Yum D.-Y., Lee Y.-P., Pan J.-G.
J. Bacteriol. 179:6566-6572(1997) [PubMed: 9352901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-28, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U97665 Genomic DNA. Translation: AAC45884.1.
PIRC38575.

3D structure databases

SMRO34215. Positions 20-290.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.99.3. 291533.

Family and domain databases

InterProIPR008168. Cyt_C_IC.
IPR009056. Cyt_c_monohaem.
IPR014353. Membr-bd_AlcDH_cyt_c.
[Graphical view]
Gene3DG3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
PIRSFPIRSF000018. Mb_ADH_cyt_c. 1 hit.
PRINTSPR00605. CYTCHROMECIC.
PROSITEPS51007. CYTC. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGADH2_PECCY
AccessionPrimary (citable) accession number: O34215
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 1, 1998
Last modified: February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents