O34215 (GADH2_PECCY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Gluconate 2-dehydrogenase cytochrome c subunit Short name=GA 2-DH cytochrome c subunit Short name=GADH cytochrome c subunit EC=1.1.99.3
Organism	Pectobacterium cypripedii (Erwinia cypripedii)
Taxonomic identifier	55209 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate.
Catalytic activity	D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor.
Cofactor	FAD.
Subunit structure	Heterotrimer.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side Potential.
Post-translational modification	Binds 3 heme groups per subunit Potential.
Sequence similarities	Contains 3 cytochrome c domains.
Biophysicochemical properties	pH dependence: Optimum pH is 5.0. Ref.1 Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro gluconate 2-dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Chain	20 – 441	422	Gluconate 2-dehydrogenase cytochrome c subunit		PRO_0000045855
Regions
	Domain	26 – 129	104	Cytochrome c 1
	Domain	173 – 289	117	Cytochrome c 2
	Domain	312 – 403	92	Cytochrome c 3
Sites
	Metal binding	44	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	192	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	329	1	Iron (heme 3 axial ligand) By similarity
	Binding site	40	1	Heme 1 (covalent) By similarity
	Binding site	43	1	Heme 1 (covalent) By similarity
	Binding site	188	1	Heme 2 (covalent) By similarity
	Binding site	191	1	Heme 2 (covalent) By similarity
	Binding site	325	1	Heme 3 (covalent) By similarity
	Binding site	328	1	Heme 3 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O34215 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0AF512CFA99DA123	FASTA	441	47,097
	10 20 30 40 50 60 MMKSILALVL GTLSFAALAD DQANDALVKR GEYLARAGDC VACHSVKGGQ PFAGGLPMAT 70 80 90 100 110 120 PIGTIYSTNI TPDKTTGIGD YSYDDFQKAV RHGVAKNGDT LYPAMPYPSY AVVSDEDMKA 130 140 150 160 170 180 LYAYFMHGVA PVAQANKDSD IPWPLSMRWP LAIWRGVFAP DVKAFQPAAQ EDPVLARGRY 190 200 210 220 230 240 LVEGLGHCGA CHTPRSITMQ EKALSNDGAH DYLSGSSAPI DGWTASNLRG DNRDGLGRWS 250 260 270 280 290 300 EDDLRQFLRY GRNDHTAAFG GMTDVVEHSL QHLSDDDITA IARYLKSLGA KDASQTVFTQ 310 320 330 340 350 360 DDQVAKALWK GDDSQTGASV YVDSCAACHK TDGSRLSALL PGAAWQPGGA GEPDPTSLIH 370 380 390 400 410 420 IVLTGGTLPG VQGAPTAITM PAFGWRLNDQ QVADVVNFIR GSWGNGAKAT VTAKDVASLR 430 440 KDETVQAHQG NADIKVLEQQ Q

References

[1]

"Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli." Yum D.-Y., Lee Y.-P., Pan J.-G. J. Bacteriol. 179:6566-6572(1997) [PubMed: 9352901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-28, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U97665 Genomic DNA. Translation: AAC45884.1.
PIR	C38575.
3D structure databases
ProteinModelPortal	O34215.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009056. Cyt_c_dom. IPR003088. Cyt_c_I. IPR008168. Cyt_C_IC. IPR014353. Membr-bd_ADH_cyt_c. [Graphical view]
Gene3D	G3DSA:1.10.760.10. Cytochrome_c_R. 3 hits.
Pfam	PF00034. Cytochrom_C. 2 hits. [Graphical view]
PIRSF	PIRSF000018. Mb_ADH_cyt_c. 1 hit.
PRINTS	PR00605. CYTCHROMECIC.
SUPFAM	SSF46626. Cytochrome_c. 3 hits.
PROSITE	PS51007. CYTC. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GADH2_PECCY

Accession

Primary (citable) accession number: O34215

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 1, 1998
Last modified:	July 27, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program