Reviewed, UniProtKB/Swiss-Prot O34214 (GADH1_PECCY)

Names and origin

Protein names	Recommended name:     Gluconate 2-dehydrogenase flavoprotein     EC=1.1.99.3 Alternative name(s):     GA 2-DH dehydrogenase subunit       Short name=GADH dehydrogenase subunit
Organism	Pectobacterium cypripedii (Erwinia cypripedii)
Taxonomic identifier	55209 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate. This subunit functions as the dehydrogenase.
Catalytic activity	D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor.
Cofactor	FAD.
Subunit structure	Heterotrimer.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side Potential.
Sequence similarities	Belongs to the GMC oxidoreductase family.
biophysicochemical properties	pH dependence: Optimum pH is 5.0. Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro gluconate 2-dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 615	593	Gluconate 2-dehydrogenase flavoprotein		PRO_0000045854

Sequences

	Sequence		Length	Mass (Da)
	O34214-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B9E1A84FD035609A	FASTA	615	67,242
	10 20 30 40 50 60 MERGERVSVP VSGYSRGEGV TVANELKKVD AVVVGFGWAG AIMAKELTEA GLNVVALERG 70 80 90 100 110 120 PHRDTYPDGA YPQSIDELTY NIRKKLFQDL SKSTVTIRHD ASQTAVPYRQ LAAFLPGTGT 130 140 150 160 170 180 GGAGLHWSGV HFRVDPVELN LRSHYEARYG KNFIPEGMTI QDFGVSYNEL EPFFDQAEKV 190 200 210 220 230 240 FGTSGSAWTI KGKMIGKEKG GNFYAPDRSS DFPLPAQKRT YSAQLFAQAA ESVGYHPYDM 250 260 270 280 290 300 PSANTSGPYT NTYGAQMGPC NFCGYCSGYA CYMYSKASPN VNILPALRQE PKFELRNNAY 310 320 330 340 350 360 VLRVNLTGDK KRATGVTYLD GQGREVVQPA DLVILSAFQF HNVHLMLLSG IGQPYNPITN 370 380 390 400 410 420 EGVVGRNFAY QNISTLKALF DKNTTTNPFI GAGGAGVAVD DFNADNFDHG PYGFVGGSPF 430 440 450 460 470 480 WVNQAGTKPV SGLPTPKGTP NWGSQWKAAV ADTYNHHISM DAHGAHQSYR ANYLDLDPNY 490 500 510 520 530 540 KNVYGQPLLR MTFDWQDNDI RMAQFMVGKM RKITEAMNPK MIIGGAKGPG THFDTTVYQT 550 560 570 580 590 600 THMSGGAIMG EDPKTSAVNR YLQSWDVPNV FVPGASAFPQ GLGYNPTGMV AALTYWSAKA 610 IREQYLKNPG PLVQA

References

[1]

"Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli." Yum D.-Y., Lee Y.-P., Pan J.-G. J. Bacteriol. 179:6566-6572(1997) [PubMed: 9352901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-37, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U97665 Genomic DNA. Translation: AAC45885.1.
PIR	B38575.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.99.3. 291533.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR000172. GMC_OxRdtase_N. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PROSITE	PS00623. GMC_OXRED_1. False negative. PS00624. GMC_OXRED_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GADH1_PECCY

Accession

Primary (citable) accession number: O34214

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)