ID   GADH3_PECCY             Reviewed;         220 AA.
AC   O34213;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 35.
DE   RecName: Full=Gluconate 2-dehydrogenase subunit 3;
DE            Short=GADH subunit 3;
DE            Short=GA 2-DH subunit 3;
DE            EC=1.1.99.3;
DE   Flags: Precursor;
OS   Pectobacterium cypripedii (Erwinia cypripedii).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=55209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-57, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004;
RX   MEDLINE=98012950; PubMed=9352901;
RA   Yum D.-Y., Lee Y.-P., Pan J.-G.;
RT   "Cloning and expression of a gene cluster encoding three subunits of
RT   membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC
RT   29267 in Escherichia coli.";
RL   J. Bacteriol. 179:6566-6572(1997).
CC   -!- FUNCTION: Part of the heterotrimer that catalyzes the conversion
CC       of D-gluconate to 2-dehydro-D-gluconate. The role of this subunit
CC       in the heterotrimer is not known.
CC   -!- CATALYTIC ACTIVITY: D-gluconate + acceptor = 2-dehydro-D-gluconate
CC       + reduced acceptor.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 5.0;
CC       Temperature dependence:
CC         Optimum temperature is 30 degrees Celsius;
CC   -!- SUBUNIT: Heterotrimer.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein;
CC       Periplasmic side (Potential).
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has been experimentally proven.
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DR   EMBL; U97665; AAC45883.1; -; Genomic_DNA.
DR   PIR; A38575; A38575.
DR   BRENDA; 1.1.99.3; 291533.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0033717; F:gluconate 2-dehydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017909; Twin_arg_translocation_Tat.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Membrane; Oxidoreductase;
KW   Signal.
FT   SIGNAL        1     42       Tat-type signal.
FT   CHAIN        43    220       Gluconate 2-dehydrogenase subunit 3.
FT                                /FTId=PRO_0000045856.
SQ   SEQUENCE   220 AA;  24368 MW;  CDDB85B110663803 CRC64;
     MSEHKNGHTR RDFLLRTITL APAMAVGSTA MGALVAPMAA GAAEQSSGSQ TARDYQPTWF
     TAEEFAFITA AVARLIPNDE RGPGALEAGV PEFIDRQMNT PYALGSNWYM QGPFNPDLPK
     ELGYQLPLVP QQIYRLGLAD ADSWSKHQHG KVFAELSGDQ QDALLSDFES GKAEFTQLPA
     KTFFSFLLQN TREGYFTRSD PRWQSGHGGL EADWLPRRTR
//