Reviewed, UniProtKB/Swiss-Prot O34213 (GADH3_PECCY)

Names and origin

Protein names	Recommended name:     Gluconate 2-dehydrogenase subunit 3       Short name=GADH subunit 3       Short name=GA 2-DH subunit 3     EC=1.1.99.3
Organism	Pectobacterium cypripedii (Erwinia cypripedii)
Taxonomic identifier	55209 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate. The role of this subunit in the heterotrimer is not known.
Catalytic activity	D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor.
Subunit structure	Heterotrimer.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic sidePotential.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Biophysicochemical properties	pH dependence: Optimum pH is 5.0. Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gluconate 2-dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 42	42	Tat-type signal
	Chain	43 – 220	178	Gluconate 2-dehydrogenase subunit 3		PRO_0000045856

Sequences

	Sequence		Length	Mass (Da)
	O34213-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CDDB85B110663803	FASTA	220	24,368
	10 20 30 40 50 60 MSEHKNGHTR RDFLLRTITL APAMAVGSTA MGALVAPMAA GAAEQSSGSQ TARDYQPTWF 70 80 90 100 110 120 TAEEFAFITA AVARLIPNDE RGPGALEAGV PEFIDRQMNT PYALGSNWYM QGPFNPDLPK 130 140 150 160 170 180 ELGYQLPLVP QQIYRLGLAD ADSWSKHQHG KVFAELSGDQ QDALLSDFES GKAEFTQLPA 190 200 210 220 KTFFSFLLQN TREGYFTRSD PRWQSGHGGL EADWLPRRTR

References

[1]

"Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli." Yum D.-Y., Lee Y.-P., Pan J.-G. J. Bacteriol. 179:6566-6572(1997) [PubMed: 9352901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-57, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U97665 Genomic DNA. Translation: AAC45883.1.
PIR	A38575.
3D structure databases
ModBase	Search...
Family and domain databases
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GADH3_PECCY

Accession

Primary (citable) accession number: O34213

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 1, 1998
Last modified:	November 4, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)