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Protein

Alginate biosynthesis sensor protein KinB

Gene

kinB

Organism
Pseudomonas aeruginosa
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Alginate biosynthesis, Sensory transduction

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Alginate biosynthesis sensor protein KinBCurated (EC:2.7.13.31 Publication)
Gene namesi
Name:kinB
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12Cytoplasmic1 PublicationAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 167Periplasmic1 PublicationAdd BLAST134
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 595Cytoplasmic1 PublicationAdd BLAST407

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi385H → K or Q: Loss of autophosphorylation. 1 Publication1
Mutagenesisi504N → Q: Loss of autophosphorylation. 1 Publication1
Mutagenesisi532D → E: Loss of autophosphorylation. 1 Publication1
Mutagenesisi532D → N: Residual autophosphorylation. 1 Publication1
Mutagenesisi560G → A: Loss of autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747781 – 595Alginate biosynthesis sensor protein KinBAdd BLAST595

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO34206.

PTM databases

iPTMnetiO34206.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5484.

Structurei

Secondary structure

1595
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 70Combined sources30
Beta strandi72 – 74Combined sources3
Helixi77 – 97Combined sources21
Helixi101 – 118Combined sources18
Helixi121 – 126Combined sources6
Beta strandi131 – 133Combined sources3
Helixi134 – 162Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KKBX-ray1.88A/B41-168[»]
ProteinModelPortaliO34206.
SMRiO34206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO34206.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini195 – 247HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini258 – 323PASCuratedAdd BLAST66
Domaini327 – 369PACCuratedAdd BLAST43
Domaini382 – 595Histidine kinasePROSITE-ProRule annotationAdd BLAST214

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ES5. Bacteria.
COG5000. LUCA.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR031909. KinB_sensor_dom.
IPR000014. PAS.
IPR013656. PAS_4.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF16767. KinB_sensor. 1 hit.
PF08448. PAS_4. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMPLPMKLR TRLFLSISAL ITVSLFGLLL GLFSVMQLGR AQEQRMSHHY
60 70 80 90 100
ATIEVSQQLR QLLGDQLVIL LRETPDGQAL ERSQNDFRRV LEQGRANTVD
110 120 130 140 150
SAEQAALDGV RDAYLQLQAH TPALLEAPMV DNDGFSEAFN GLRLRLQDLQ
160 170 180 190 200
QLALAGISDA ETSARHRAYL VAGLLGLVGV AILLIGFVTA HSIARRFGAP
210 220 230 240 250
IETLARAADR IGEGDFDVTL PMTNVAEVGQ LTRRFGLMAE ALRQYRKTSV
260 270 280 290 300
EEVLSGERRL QAVLDSIDDG LVIFDNQGRI EHANPVAIRQ LFVSNDPHGK
310 320 330 340 350
RIDEILSDVD VQEAVEKALL GEVQDEAMPD LVVDVAGESR LLAWSLYPVT
360 370 380 390 400
HPGGHSVGAV LVVRDVTEQR AFERVRSEFV LRASHELRTP VTGMQMAFSL
410 420 430 440 450
LRERLDFPAE SREADLIQTV DEEMSRLVLL INDLLNFSRY QTGMQKLELA
460 470 480 490 500
SCDLVDLLTQ AQQRFIPKGE ARRVSLQLEL GDELPRLQLD RLQIERVIDN
510 520 530 540 550
LLENALRHSS EGGQIHLQAR RQGDRVLIAV EDNGEGIPFS QQGRIFEPFV
560 570 580 590
QVGRKKGGAG LGLELCKEII QLHGGRIAVR SQPGQGARFY MLLPV
Length:595
Mass (Da):66,072
Last modified:January 1, 1998 - v1
Checksum:iBA12FC7E3120F048
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97063 Genomic DNA. Translation: AAB68790.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97063 Genomic DNA. Translation: AAB68790.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KKBX-ray1.88A/B41-168[»]
ProteinModelPortaliO34206.
SMRiO34206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5484.

PTM databases

iPTMnetiO34206.

Proteomic databases

PaxDbiO34206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105ES5. Bacteria.
COG5000. LUCA.

Enzyme and pathway databases

BRENDAi2.7.13.3. 5087.

Miscellaneous databases

EvolutionaryTraceiO34206.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR031909. KinB_sensor_dom.
IPR000014. PAS.
IPR013656. PAS_4.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF16767. KinB_sensor. 1 hit.
PF08448. PAS_4. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKINB_PSEAI
AccessioniPrimary (citable) accession number: O34206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.