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O34204 (TOP1_FERIS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
OrganismFervidobacterium islandicum
Taxonomic identifier2423 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

Protein attributes

Sequence length699 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 699699DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145148

Regions

Domain38 – 146109Toprim
Zinc finger601 – 62424C4-type HAMAP-Rule MF_00952
Region194 – 1996Interaction with DNA By similarity

Sites

Active site3241O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding441Magnesium 1; catalytic By similarity
Metal binding1151Magnesium 1; catalytic By similarity
Metal binding1151Magnesium 2 By similarity
Metal binding1171Magnesium 2 By similarity
Site681Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site1711Interaction with DNA By similarity
Site1741Interaction with DNA By similarity
Site1791Interaction with DNA By similarity
Site1861Interaction with DNA By similarity
Site3261Interaction with DNA By similarity
Site5151Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
O34204 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9137E85A8A01FD2C

FASTA69979,612
        10         20         30         40         50         60 
MAKSKVVEKD KKNELDNQSA DIELKGQSKN EESKGGKKKV IIVESPAKAK TIERILGNDY 

        70         80         90        100        110        120 
QVISSKGHIR DLPQKQFGVD LNSLKLDFEI IPGKESVVEQ IKKMTSGKEV LLPSDQDREG 

       130        140        150        160        170        180 
EAIAWHLSTI LGVKGKNIIT FTEITERAIK EAVKNPREID MNKVNAQLAR RVLDRIVGYM 

       190        200        210        220        230        240 
ISPLLWRIIK DARSAGRVQS AALKIICERE RERYRFVPQK YFKVWIDIAG LKAYLTKVDG 

       250        260        270        280        290        300 
KKIKPTDITE EISKDVLQKV KSVRLVDIDV KEVRKNPPAP FITSTLQQDA ASKLGFPVSK 

       310        320        330        340        350        360 
TMKIAQELYE GVDTKEGHTA FITYMRTDST RVSDVAKEAA EKFILKNFGR EYLNESSEVA 

       370        380        390        400        410        420 
SKKGSKTKGK VQDAHECIRP VDVNITPEKA KELLDKDHHK LYELIWKRFI ASQMSSAIYK 

       430        440        450        460        470        480 
QYSYDFESGK YVFEASIRER IFDGFEKVYT IDNEPSEKHK ELKVDQEYLV EPKSAEAQTT 

       490        500        510        520        530        540 
PPDRYTEASL VKTLEMEGIG RPSTYATIIQ TLLDRGYVVK KRKTLIPTIL GFVVNHYLEQ 

       550        560        570        580        590        600 
RFPDIVDKGF TAEMEKELDE VENGKKDWKE VVKSFLKEFN KDLERAKNEF FAIDFDTDIT 

       610        620        630        640        650        660 
CEDCSGNYKL KVGKYGLYLH CPNCKTNKAL KSDVFGVIDG NKLYVLEEQE SQEENGEKNS 

       670        680        690 
VQSEESSANS GNRKFYRKRR TSGSKKSSTK SASSKAKKK 

« Hide

References

[1]"DNA topoisomerases I from thermophilic eubacteria: cloning and sequencing of the DNA topoisomerase I from Fervidobacterium islandicum."
Kaltoum H., Portemer C., Confalonieri F., Duguet M., Bouthier de La Tour C.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U97022 Genomic DNA. Translation: AAB68788.1.

3D structure databases

ProteinModelPortalO34204.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_FERIS
AccessionPrimary (citable) accession number: O34204
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families