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Protein

DNA topoisomerase 1

Gene

topA

Organism
Fervidobacterium islandicum
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Magnesium 1; catalyticUniRule annotation1
Metal bindingi115Magnesium 1; catalyticUniRule annotation1
Metal bindingi115Magnesium 2UniRule annotation1
Metal bindingi117Magnesium 2UniRule annotation1
Active sitei324O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri601 – 624C4-typeAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
OrganismiFervidobacterium islandicum
Taxonomic identifieri2423 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeFervidobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451481 – 699DNA topoisomerase 1Add BLAST699

Proteomic databases

PRIDEiO34204

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei68Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei171Interaction with DNAUniRule annotation1
Sitei174Interaction with DNAUniRule annotation1
Sitei179Interaction with DNAUniRule annotation1
Sitei186Interaction with DNAUniRule annotation1
Sitei326Interaction with DNAUniRule annotation1
Sitei515Interaction with DNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliO34204
SMRiO34204
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 146ToprimUniRule annotationAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri601 – 624C4-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 2 hits
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

O34204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSKVVEKD KKNELDNQSA DIELKGQSKN EESKGGKKKV IIVESPAKAK
60 70 80 90 100
TIERILGNDY QVISSKGHIR DLPQKQFGVD LNSLKLDFEI IPGKESVVEQ
110 120 130 140 150
IKKMTSGKEV LLPSDQDREG EAIAWHLSTI LGVKGKNIIT FTEITERAIK
160 170 180 190 200
EAVKNPREID MNKVNAQLAR RVLDRIVGYM ISPLLWRIIK DARSAGRVQS
210 220 230 240 250
AALKIICERE RERYRFVPQK YFKVWIDIAG LKAYLTKVDG KKIKPTDITE
260 270 280 290 300
EISKDVLQKV KSVRLVDIDV KEVRKNPPAP FITSTLQQDA ASKLGFPVSK
310 320 330 340 350
TMKIAQELYE GVDTKEGHTA FITYMRTDST RVSDVAKEAA EKFILKNFGR
360 370 380 390 400
EYLNESSEVA SKKGSKTKGK VQDAHECIRP VDVNITPEKA KELLDKDHHK
410 420 430 440 450
LYELIWKRFI ASQMSSAIYK QYSYDFESGK YVFEASIRER IFDGFEKVYT
460 470 480 490 500
IDNEPSEKHK ELKVDQEYLV EPKSAEAQTT PPDRYTEASL VKTLEMEGIG
510 520 530 540 550
RPSTYATIIQ TLLDRGYVVK KRKTLIPTIL GFVVNHYLEQ RFPDIVDKGF
560 570 580 590 600
TAEMEKELDE VENGKKDWKE VVKSFLKEFN KDLERAKNEF FAIDFDTDIT
610 620 630 640 650
CEDCSGNYKL KVGKYGLYLH CPNCKTNKAL KSDVFGVIDG NKLYVLEEQE
660 670 680 690
SQEENGEKNS VQSEESSANS GNRKFYRKRR TSGSKKSSTK SASSKAKKK
Length:699
Mass (Da):79,612
Last modified:January 1, 1998 - v1
Checksum:i9137E85A8A01FD2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97022 Genomic DNA Translation: AAB68788.1

Similar proteinsi

Entry informationi

Entry nameiTOP1_FERIS
AccessioniPrimary (citable) accession number: O34204
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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