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O34204

- TOP1_FERIS

UniProt

O34204 - TOP1_FERIS

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Protein
DNA topoisomerase 1
Gene
topA
Organism
Fervidobacterium islandicum
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Magnesium 1; catalytic By similarity
Sitei68 – 681Interaction with DNA By similarity
Metal bindingi115 – 1151Magnesium 1; catalytic By similarity
Metal bindingi115 – 1151Magnesium 2 By similarity
Metal bindingi117 – 1171Magnesium 2 By similarity
Sitei170 – 1701Interaction with DNA By similarity
Sitei171 – 1711Interaction with DNA By similarity
Sitei174 – 1741Interaction with DNA By similarity
Sitei179 – 1791Interaction with DNA By similarity
Sitei186 – 1861Interaction with DNA By similarity
Active sitei324 – 3241O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei326 – 3261Interaction with DNA By similarity
Sitei515 – 5151Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri601 – 62424C4-typeUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
OrganismiFervidobacterium islandicum
Taxonomic identifieri2423 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699DNA topoisomerase 1UniRule annotation
PRO_0000145148Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO34204.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 146109Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1996Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 2 hits.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34204-1 [UniParc]FASTAAdd to Basket

« Hide

MAKSKVVEKD KKNELDNQSA DIELKGQSKN EESKGGKKKV IIVESPAKAK    50
TIERILGNDY QVISSKGHIR DLPQKQFGVD LNSLKLDFEI IPGKESVVEQ 100
IKKMTSGKEV LLPSDQDREG EAIAWHLSTI LGVKGKNIIT FTEITERAIK 150
EAVKNPREID MNKVNAQLAR RVLDRIVGYM ISPLLWRIIK DARSAGRVQS 200
AALKIICERE RERYRFVPQK YFKVWIDIAG LKAYLTKVDG KKIKPTDITE 250
EISKDVLQKV KSVRLVDIDV KEVRKNPPAP FITSTLQQDA ASKLGFPVSK 300
TMKIAQELYE GVDTKEGHTA FITYMRTDST RVSDVAKEAA EKFILKNFGR 350
EYLNESSEVA SKKGSKTKGK VQDAHECIRP VDVNITPEKA KELLDKDHHK 400
LYELIWKRFI ASQMSSAIYK QYSYDFESGK YVFEASIRER IFDGFEKVYT 450
IDNEPSEKHK ELKVDQEYLV EPKSAEAQTT PPDRYTEASL VKTLEMEGIG 500
RPSTYATIIQ TLLDRGYVVK KRKTLIPTIL GFVVNHYLEQ RFPDIVDKGF 550
TAEMEKELDE VENGKKDWKE VVKSFLKEFN KDLERAKNEF FAIDFDTDIT 600
CEDCSGNYKL KVGKYGLYLH CPNCKTNKAL KSDVFGVIDG NKLYVLEEQE 650
SQEENGEKNS VQSEESSANS GNRKFYRKRR TSGSKKSSTK SASSKAKKK 699
Length:699
Mass (Da):79,612
Last modified:January 1, 1998 - v1
Checksum:i9137E85A8A01FD2C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97022 Genomic DNA. Translation: AAB68788.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97022 Genomic DNA. Translation: AAB68788.1 .

3D structure databases

ProteinModelPortali O34204.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 2 hits.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA topoisomerases I from thermophilic eubacteria: cloning and sequencing of the DNA topoisomerase I from Fervidobacterium islandicum."
    Kaltoum H., Portemer C., Confalonieri F., Duguet M., Bouthier de La Tour C.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTOP1_FERIS
AccessioniPrimary (citable) accession number: O34204
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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