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O34204

- TOP1_FERIS

UniProt

O34204 - TOP1_FERIS

Protein

DNA topoisomerase 1

Gene

topA

Organism
Fervidobacterium islandicum
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Magnesium 1; catalyticUniRule annotation
    Sitei68 – 681Interaction with DNAUniRule annotation
    Metal bindingi115 – 1151Magnesium 1; catalyticUniRule annotation
    Metal bindingi115 – 1151Magnesium 2UniRule annotation
    Metal bindingi117 – 1171Magnesium 2UniRule annotation
    Sitei170 – 1701Interaction with DNAUniRule annotation
    Sitei171 – 1711Interaction with DNAUniRule annotation
    Sitei174 – 1741Interaction with DNAUniRule annotation
    Sitei179 – 1791Interaction with DNAUniRule annotation
    Sitei186 – 1861Interaction with DNAUniRule annotation
    Active sitei324 – 3241O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei326 – 3261Interaction with DNAUniRule annotation
    Sitei515 – 5151Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri601 – 62424C4-typeAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    OrganismiFervidobacterium islandicum
    Taxonomic identifieri2423 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 699699DNA topoisomerase 1PRO_0000145148Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO34204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 146109ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni194 – 1996Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri601 – 62424C4-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 2 hits.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O34204-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKSKVVEKD KKNELDNQSA DIELKGQSKN EESKGGKKKV IIVESPAKAK    50
    TIERILGNDY QVISSKGHIR DLPQKQFGVD LNSLKLDFEI IPGKESVVEQ 100
    IKKMTSGKEV LLPSDQDREG EAIAWHLSTI LGVKGKNIIT FTEITERAIK 150
    EAVKNPREID MNKVNAQLAR RVLDRIVGYM ISPLLWRIIK DARSAGRVQS 200
    AALKIICERE RERYRFVPQK YFKVWIDIAG LKAYLTKVDG KKIKPTDITE 250
    EISKDVLQKV KSVRLVDIDV KEVRKNPPAP FITSTLQQDA ASKLGFPVSK 300
    TMKIAQELYE GVDTKEGHTA FITYMRTDST RVSDVAKEAA EKFILKNFGR 350
    EYLNESSEVA SKKGSKTKGK VQDAHECIRP VDVNITPEKA KELLDKDHHK 400
    LYELIWKRFI ASQMSSAIYK QYSYDFESGK YVFEASIRER IFDGFEKVYT 450
    IDNEPSEKHK ELKVDQEYLV EPKSAEAQTT PPDRYTEASL VKTLEMEGIG 500
    RPSTYATIIQ TLLDRGYVVK KRKTLIPTIL GFVVNHYLEQ RFPDIVDKGF 550
    TAEMEKELDE VENGKKDWKE VVKSFLKEFN KDLERAKNEF FAIDFDTDIT 600
    CEDCSGNYKL KVGKYGLYLH CPNCKTNKAL KSDVFGVIDG NKLYVLEEQE 650
    SQEENGEKNS VQSEESSANS GNRKFYRKRR TSGSKKSSTK SASSKAKKK 699
    Length:699
    Mass (Da):79,612
    Last modified:January 1, 1998 - v1
    Checksum:i9137E85A8A01FD2C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U97022 Genomic DNA. Translation: AAB68788.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U97022 Genomic DNA. Translation: AAB68788.1 .

    3D structure databases

    ProteinModelPortali O34204.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 2 hits.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA topoisomerases I from thermophilic eubacteria: cloning and sequencing of the DNA topoisomerase I from Fervidobacterium islandicum."
      Kaltoum H., Portemer C., Confalonieri F., Duguet M., Bouthier de La Tour C.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiTOP1_FERIS
    AccessioniPrimary (citable) accession number: O34204
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3