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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Trichodesmium erythraeum (strain IMS101)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.By similarity

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi131Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron protein (EC:1.18.6.1)
Alternative name(s):
Nitrogenase Fe protein
Nitrogenase component II
Nitrogenase reductase
Gene namesi
Name:nifH
Ordered Locus Names:Tery_4136
OrganismiTrichodesmium erythraeum (strain IMS101)
Taxonomic identifieri203124 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesMicrocoleaceaeTrichodesmium
Proteomesi
  • UP000008878 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395331 – 296Nitrogenase iron proteinAdd BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei100ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseBy similarity1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-100 inactivates the nitrogenase reductase and regulates nitrogenase activity.By similarity

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi203124.Tery_4136.

Structurei

3D structure databases

ProteinModelPortaliO34106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.Curated

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQIAFYGKG GIGKSTTSQN TLAAMANRHG QRIMIVGCDP KADSTRLILN
60 70 80 90 100
AKAQTTVLHV AAERGAVEDV ELDEVLKPGF GGIKCVESGG PEPGVGCAGR
110 120 130 140 150
GIITAINFLE EEGAYTDLDF VSYDVLGDVV CGGFAMPIRE NKAQEIYIVC
160 170 180 190 200
SGEMMAMYAA NNIARGVLKY AHAGGVRLGG LICNSRKVDR ETELIENLAA
210 220 230 240 250
RLNTQMIHFV PRDNVVQRAE IRRMTVEQYA PEDNQAQEYD QLAQKIINNE
260 270 280 290
KLTIPTPLEM DELEELLIEF GLLGDEEDRQ KQIAAQDAAI KATAAK
Length:296
Mass (Da):32,231
Last modified:January 1, 1998 - v1
Checksum:i84ECC20633078795
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90952 Genomic DNA. Translation: AAB70120.1.
AF016484 Genomic DNA. Translation: AAD03796.1.
AF167538 Genomic DNA. Translation: AAF82637.1.
CP000393 Genomic DNA. Translation: ABG53144.1.
RefSeqiWP_011613474.1. NC_008312.1.

Genome annotation databases

EnsemblBacteriaiABG53144; ABG53144; Tery_4136.
KEGGiter:Tery_4136.
PATRICi23993689. VBITriEry99848_5250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90952 Genomic DNA. Translation: AAB70120.1.
AF016484 Genomic DNA. Translation: AAD03796.1.
AF167538 Genomic DNA. Translation: AAF82637.1.
CP000393 Genomic DNA. Translation: ABG53144.1.
RefSeqiWP_011613474.1. NC_008312.1.

3D structure databases

ProteinModelPortaliO34106.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203124.Tery_4136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG53144; ABG53144; Tery_4136.
KEGGiter:Tery_4136.
PATRICi23993689. VBITriEry99848_5250.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228826.
KOiK02588.
OMAiNKMFVIP.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFH_TRIEI
AccessioniPrimary (citable) accession number: O34106
Secondary accession number(s): Q10X80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.