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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Trichodesmium erythraeum (strain IMS101)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.By similarity

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi131Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTERY203124:G1G6S-4222-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron protein (EC:1.18.6.1)
Alternative name(s):
Nitrogenase Fe protein
Nitrogenase component II
Nitrogenase reductase
Gene namesi
Name:nifH
Ordered Locus Names:Tery_4136
OrganismiTrichodesmium erythraeum (strain IMS101)
Taxonomic identifieri203124 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesMicrocoleaceaeTrichodesmium
Proteomesi
  • UP000008878 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395331 – 296Nitrogenase iron proteinAdd BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei100ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseBy similarity1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-100 inactivates the nitrogenase reductase and regulates nitrogenase activity.By similarity

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi203124.Tery_4136

Structurei

3D structure databases

ProteinModelPortaliO34106
SMRiO34106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.Curated

Phylogenomic databases

eggNOGiENOG4105DSM Bacteria
COG1348 LUCA
HOGENOMiHOG000228826
KOiK02588
OMAiEMGQRIM
OrthoDBiPOG091H0230

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01287 nifH, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

O34106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQIAFYGKG GIGKSTTSQN TLAAMANRHG QRIMIVGCDP KADSTRLILN
60 70 80 90 100
AKAQTTVLHV AAERGAVEDV ELDEVLKPGF GGIKCVESGG PEPGVGCAGR
110 120 130 140 150
GIITAINFLE EEGAYTDLDF VSYDVLGDVV CGGFAMPIRE NKAQEIYIVC
160 170 180 190 200
SGEMMAMYAA NNIARGVLKY AHAGGVRLGG LICNSRKVDR ETELIENLAA
210 220 230 240 250
RLNTQMIHFV PRDNVVQRAE IRRMTVEQYA PEDNQAQEYD QLAQKIINNE
260 270 280 290
KLTIPTPLEM DELEELLIEF GLLGDEEDRQ KQIAAQDAAI KATAAK
Length:296
Mass (Da):32,231
Last modified:January 1, 1998 - v1
Checksum:i84ECC20633078795
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90952 Genomic DNA Translation: AAB70120.1
AF016484 Genomic DNA Translation: AAD03796.1
AF167538 Genomic DNA Translation: AAF82637.1
CP000393 Genomic DNA Translation: ABG53144.1
RefSeqiWP_011613474.1, NC_008312.1

Genome annotation databases

EnsemblBacteriaiABG53144; ABG53144; Tery_4136
KEGGiter:Tery_4136

Entry informationi

Entry nameiNIFH_TRIEI
AccessioniPrimary (citable) accession number: O34106
Secondary accession number(s): Q10X80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health