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O34104 (T2S1_LACLC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme ScrFI

Short name=R.ScrFI
EC=3.1.21.4
Alternative name(s):
Endonuclease ScrFI
Type II restriction enzyme ScrFI
Gene names
Name:scrFIR
OrganismLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifier1359 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCNGG and cleaves after C-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Type-2 restriction enzyme ScrFI
PRO_0000077360

Sequences

Sequence LengthMass (Da)Tools
O34104 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 862D505647DF92BE

FASTA27131,467
        10         20         30         40         50         60 
MNPNKERLIE IFRSNVKGRI PDISGRNIRH DGRWGHWLEE RFGISANADN HADILGYELK 

        70         80         90        100        110        120 
NEATSGKTTF GDWSANEYIF KTPPYNSLFS GSTASEKQNA FCRMFGKPNE AKNGRFSWSG 

       130        140        150        160        170        180 
SPIPKIWQYN SFGQIMVIEE NLDIVIYYSF SQDLRYNKFE IIPPQLQHDE IQIARWYGVA 

       190        200        210        220        230        240 
NPLLSRRGKT LKDKLEDKFN DLGWFTCTTD SIGAYDKICF GRPITFENWI NLVDSGIVYF 

       250        260        270 
DSGMYEGNKR PYSQWRADNS YWNSLITDCH Q 

« Hide

References

[1]"Molecular characterization of the restriction endonuclease gene (scrFIR) associated with the ScrFI restriction/modification system from Lactococcus lactis subsp. cremoris UC503."
Twomey D.P., Gabillet N., Daly C., Fitzgerald G.F.
Microbiology 143:2277-2286(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: UC503.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89998 Genomic DNA. Translation: AAB66695.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1639. ScrFI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019063. Restrct_endonuc_II_LlaMI.
[Graphical view]
PfamPF09562. RE_LlaMI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S1_LACLC
AccessionPrimary (citable) accession number: O34104
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries