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O34104

- T2S1_LACLC

UniProt

O34104 - T2S1_LACLC

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Protein

Type-2 restriction enzyme ScrFI

Gene
scrFIR
Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence CCNGG and cleaves after C-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1639. ScrFI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme ScrFI (EC:3.1.21.4)
Short name:
R.ScrFI
Alternative name(s):
Endonuclease ScrFI
Type II restriction enzyme ScrFI
Gene namesi
Name:scrFIR
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Type-2 restriction enzyme ScrFIPRO_0000077360Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019063. Restrct_endonuc_II_LlaMI.
[Graphical view]
PfamiPF09562. RE_LlaMI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O34104-1 [UniParc]FASTAAdd to Basket

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MNPNKERLIE IFRSNVKGRI PDISGRNIRH DGRWGHWLEE RFGISANADN    50
HADILGYELK NEATSGKTTF GDWSANEYIF KTPPYNSLFS GSTASEKQNA 100
FCRMFGKPNE AKNGRFSWSG SPIPKIWQYN SFGQIMVIEE NLDIVIYYSF 150
SQDLRYNKFE IIPPQLQHDE IQIARWYGVA NPLLSRRGKT LKDKLEDKFN 200
DLGWFTCTTD SIGAYDKICF GRPITFENWI NLVDSGIVYF DSGMYEGNKR 250
PYSQWRADNS YWNSLITDCH Q 271
Length:271
Mass (Da):31,467
Last modified:January 1, 1998 - v1
Checksum:i862D505647DF92BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U89998 Genomic DNA. Translation: AAB66695.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U89998 Genomic DNA. Translation: AAB66695.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1639. ScrFI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019063. Restrct_endonuc_II_LlaMI.
[Graphical view ]
Pfami PF09562. RE_LlaMI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of the restriction endonuclease gene (scrFIR) associated with the ScrFI restriction/modification system from Lactococcus lactis subsp. cremoris UC503."
    Twomey D.P., Gabillet N., Daly C., Fitzgerald G.F.
    Microbiology 143:2277-2286(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UC503.

Entry informationi

Entry nameiT2S1_LACLC
AccessioniPrimary (citable) accession number: O34104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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