O34104 (T2S1_LACLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme ScrFI
Short name=R.ScrFI
EC=3.1.21.4

Alternative name(s):
Endonuclease ScrFI
Type II restriction enzyme ScrFI

Gene names

Name:

scrFIR

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCNGG and cleaves after C-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Type-2 restriction enzyme ScrFI		PRO_0000077360

Sequences

	Sequence		Length	Mass (Da)
	O34104 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 862D505647DF92BE	FASTA	271	31,467
	10 20 30 40 50 60 MNPNKERLIE IFRSNVKGRI PDISGRNIRH DGRWGHWLEE RFGISANADN HADILGYELK 70 80 90 100 110 120 NEATSGKTTF GDWSANEYIF KTPPYNSLFS GSTASEKQNA FCRMFGKPNE AKNGRFSWSG 130 140 150 160 170 180 SPIPKIWQYN SFGQIMVIEE NLDIVIYYSF SQDLRYNKFE IIPPQLQHDE IQIARWYGVA 190 200 210 220 230 240 NPLLSRRGKT LKDKLEDKFN DLGWFTCTTD SIGAYDKICF GRPITFENWI NLVDSGIVYF 250 260 270 DSGMYEGNKR PYSQWRADNS YWNSLITDCH Q

References

[1]

"Molecular characterization of the restriction endonuclease gene (scrFIR) associated with the ScrFI restriction/modification system from Lactococcus lactis subsp. cremoris UC503." Twomey D.P., Gabillet N., Daly C., Fitzgerald G.F. Microbiology 143:2277-2286(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UC503.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U89998 Genomic DNA. Translation: AAB66695.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1639. ScrFI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019063. Restrct_endonuc_II_LlaMI. [Graphical view]
Pfam	PF09562. RE_LlaMI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_LACLC

Accession

Primary (citable) accession number: O34104

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program