Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O34104

- T2S1_LACLC

UniProt

O34104 - T2S1_LACLC

Protein

Type-2 restriction enzyme ScrFI

Gene

scrFIR

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 47 (16 Apr 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Recognizes the double-stranded sequence CCNGG and cleaves after C-2.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1639. ScrFI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme ScrFI (EC:3.1.21.4)
    Short name:
    R.ScrFI
    Alternative name(s):
    Endonuclease ScrFI
    Type II restriction enzyme ScrFI
    Gene namesi
    Name:scrFIR
    OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
    Taxonomic identifieri1359 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 271271Type-2 restriction enzyme ScrFIPRO_0000077360Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019063. Restrct_endonuc_II_LlaMI.
    [Graphical view]
    PfamiPF09562. RE_LlaMI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O34104-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPNKERLIE IFRSNVKGRI PDISGRNIRH DGRWGHWLEE RFGISANADN    50
    HADILGYELK NEATSGKTTF GDWSANEYIF KTPPYNSLFS GSTASEKQNA 100
    FCRMFGKPNE AKNGRFSWSG SPIPKIWQYN SFGQIMVIEE NLDIVIYYSF 150
    SQDLRYNKFE IIPPQLQHDE IQIARWYGVA NPLLSRRGKT LKDKLEDKFN 200
    DLGWFTCTTD SIGAYDKICF GRPITFENWI NLVDSGIVYF DSGMYEGNKR 250
    PYSQWRADNS YWNSLITDCH Q 271
    Length:271
    Mass (Da):31,467
    Last modified:January 1, 1998 - v1
    Checksum:i862D505647DF92BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89998 Genomic DNA. Translation: AAB66695.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89998 Genomic DNA. Translation: AAB66695.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1639. ScrFI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019063. Restrct_endonuc_II_LlaMI.
    [Graphical view ]
    Pfami PF09562. RE_LlaMI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the restriction endonuclease gene (scrFIR) associated with the ScrFI restriction/modification system from Lactococcus lactis subsp. cremoris UC503."
      Twomey D.P., Gabillet N., Daly C., Fitzgerald G.F.
      Microbiology 143:2277-2286(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: UC503.

    Entry informationi

    Entry nameiT2S1_LACLC
    AccessioniPrimary (citable) accession number: O34104
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: April 16, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3