O33952 (UDG8_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 6-dehydrogenase
Short name=UDP-Glc dehydrogenase
Short name=UDP-GlcDH
Short name=UDPGDH
EC=1.1.1.22

Gene names

Name:

ugd

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Post-translational modification	Phosphorylated on a tyrosine residue. It results in a significant increase of the dehydrogenase activity By similarity.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	UDP-glucuronate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	UDP-glucose 6-dehydrogenase		PRO_0000074046
Regions
	Nucleotide binding	2 – 19	18	NAD Potential
Sites
	Active site	253	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33952 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D30E622AA6489AC9	FASTA	388	43,549
	10 20 30 40 50 60 MKITISGTGY VGLSNGILIA QNHEVVALDI VQAKVDMLNK KQSPIVDKEI EEYLATKDLN 70 80 90 100 110 120 FRATTDKYDA YKNADYVIIA TPTDYDPKTN YFNTSSVEAV IRDVTEINPN AVMVIKSTIP 130 140 150 160 170 180 VGFTKSIKER LGIDNLIFSP EFLREGKALY DNLHPSRIVI GERSERAERF AALLQEGAIK 190 200 210 220 230 240 QNIPTLFTDS TEAEAIKLFA NTYLAMRVAY FNELDSYAES LGLNTRQIIE GVCLDPRIGN 250 260 270 280 290 300 HYNNPSFGYG GYCLPKDTKQ LLANYQSVPN NLISAIVDAN RTRKDFIADA ILSRKPQVVG 310 320 330 340 350 360 IYRLIMKSGS DNFRASSIQG IMKRIKAKGV EVIIYEPVMK EDSFFNSRLE RDLATFKQQA 370 380 DVIISNRMAE ELKDVADKVY TRDLFGSD

References

[1]

"Molecular and functional analysis of genes required for expression of group IB K antigens in Escherichia coli: characterization of the his-region containing gene clusters for multiple cell-surface polysaccharides." Amor P.A., Whitfield C. Mol. Microbiol. 26:145-161(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O8:K40 / 2775.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U78086 Genomic DNA. Translation: AAC45829.1.
3D structure databases
ProteinModelPortal	O33952.
SMR	O33952. Positions 1-388.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00030. UPA00038; UER00491.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 2 hits.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMART	SM00984. UDPG_MGDP_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UDG8_ECOLX

Accession

Primary (citable) accession number: O33952

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program